Pharmacology Lab Experiments
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Questions and Answers

What is the main purpose of the pharmacology lab?

To understand how to administer drugs, the mechanism of how drugs treat diseases, and how the body reacts in the presence of drugs.

What are the two types of antibiotics based on their activity?

Bacteriostatic and bactericide.

What are some examples of bacteriostatic antibiotics?

Tetracycline and penicillin.

How do bactericides work?

<p>Bactericides kill the bacteria, but their activity is concentration dependent and may have a static effect at low concentration.</p> Signup and view all the answers

What is the minimum inhibitory concentration (MIC) of an antibiotic?

<p>The lowest concentration of a substance that can inhibit the growth of bacteria.</p> Signup and view all the answers

How is the MIC used to determine the potency of potential antimicrobial substances?

<p>The MIC value is used to show the potency of potential antimicrobial substance and can indicate resistance if the MIC is higher than the standard value.</p> Signup and view all the answers

What are the two widely used methods to determine the MIC?

<p>Agar-disk diffusion method and dilution method.</p> Signup and view all the answers

How is the MIC value determined in the agar-disk diffusion method?

<p>The lowest concentration by which there is no visible inhibition growth shows the MIC value.</p> Signup and view all the answers

What does the dilution method involve in determining the MIC value?

<p>The dilution method involves adding antibiotic to the media at different concentrations and then adding the bacteria culture to determine the minimum concentration with no visible growth of bacteria.</p> Signup and view all the answers

What is the purpose of measuring the optical density (OD) of media-containing antibiotic inoculated with bacteria?

<p>To determine whether the activity of the antibiotic is bactericidal or bacteriostatic.</p> Signup and view all the answers

Pharmacology is defined as the study of drugs and their effects on life processes.

<p>True</p> Signup and view all the answers

Bacteriostatic antibiotics kill the bacteria.

<p>False</p> Signup and view all the answers

The main purpose of pharmacology lab is to understand how drugs treat diseases and how the body reacts to drugs.

<p>True</p> Signup and view all the answers

Antibiotics may act as both bacteriostatic and bactericide depending on their concentration.

<p>True</p> Signup and view all the answers

Aminoglycoside is an example of an antibiotic with bacteriostatic activity.

<p>False</p> Signup and view all the answers

The minimum inhibitory concentration (MIC) is defined as the lowest concentration of a substance that can promote the growth of bacteria.

<p>False</p> Signup and view all the answers

The agar-disk diffusion method is used to determine the MIC value by placing paper disks impregnated with antibiotic solution on agar plates and measuring the growth of bacteria.

<p>False</p> Signup and view all the answers

The dilution method involves adding bacteria culture to media containing antibiotics at different concentrations and then measuring the minimum concentration where there is no visible growth of bacteria.

<p>True</p> Signup and view all the answers

The MIC value can indicate resistance if it is lower than the standard value for antimicrobial substances.

<p>False</p> Signup and view all the answers

The effect of an antibiotic may vary depending on the target species, as an antibiotic can have cidal activity against one species and static activity against another species.

<p>True</p> Signup and view all the answers

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