Pharmaceutical Applications of DNA Technology Quiz

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What is the primary purpose of cleaving DNA into fragments of defined length or with specific endpoints?

To reduce the complexity of the DNA sequence

To facilitate recombinant DNA technology (correct)

To increase the size of the DNA molecule

To make the DNA more aesthetically pleasing

What is the primary function of DNA ligase in the process of creating a recombinant DNA molecule?

To cleave the DNA into fragments

To separate the DNA strands

To introduce the recombinant DNA into a host cell

To join the insert DNA to the vector DNA (correct)

Which of the following is a common method for introducing recombinant DNA into a host cell?

Genetic transformation (correct)

Cryogenic freezing

Microinjection

Electrocution

What is the primary role of a cloning vector in the process of expressing a recombinant DNA molecule?

To allow for the replication and expression of the insert DNA Signup and view all the answers

Which of the following is a key step in the process of creating a recombinant DNA molecule?

Ligation of DNA fragments Signup and view all the answers

What is the primary purpose of synthesizing target DNA in vitro in the context of recombinant DNA technology?

To provide a source of the DNA of interest for cloning and expression Signup and view all the answers

What is the main purpose of using genetic markers in vectors?

To allow for the selection of host cells that have taken up foreign DNA Signup and view all the answers

Which of the following techniques is used to transfer plasmid DNA into bacteria?

Heat shock treatment Signup and view all the answers

What is the purpose of the 'pre-treatment' step in the heat shock transformation method?

To increase the pore sizes of the plasma membranes Signup and view all the answers

Which of the following is the most difficult step in the identification of a particular DNA fragment?

Screening a large number of recombinant DNA clones Signup and view all the answers

What is the purpose of the 'gene gun' used in the biolistic method?

To fire DNA-coated pellets on plant tissues Signup and view all the answers

What is the main purpose of the 'selection' step in the identification of recombinant DNA clones?

To identify the host cells that have taken up the foreign DNA Signup and view all the answers

Which of the following is NOT a therapeutic protein produced commercially from cloned genes?

Antibiotics Signup and view all the answers

What is the purpose of developing herbicide or pesticide resistant crops?

Both A and B Signup and view all the answers

Which of the following is NOT a potential application of recombinant DNA technology in agriculture?

Generating crops with increased nutritional value Signup and view all the answers

What is the purpose of introducing an antifreeze gene from cold water fish into plants like tobacco and potato?

To improve cold tolerance Signup and view all the answers

Which of the following is NOT mentioned as an application of recombinant DNA technology in medicine?

Treatment of infectious diseases Signup and view all the answers

What is the primary purpose of developing 'Golden rice' through genetic modification?

To prevent vitamin A deficiency Signup and view all the answers