Podcast
Questions and Answers
What is the primary purpose of cleaving DNA into fragments of defined length or with specific endpoints?
What is the primary purpose of cleaving DNA into fragments of defined length or with specific endpoints?
- To reduce the complexity of the DNA sequence
- To facilitate recombinant DNA technology (correct)
- To increase the size of the DNA molecule
- To make the DNA more aesthetically pleasing
What is the primary function of DNA ligase in the process of creating a recombinant DNA molecule?
What is the primary function of DNA ligase in the process of creating a recombinant DNA molecule?
- To cleave the DNA into fragments
- To separate the DNA strands
- To introduce the recombinant DNA into a host cell
- To join the insert DNA to the vector DNA (correct)
Which of the following is a common method for introducing recombinant DNA into a host cell?
Which of the following is a common method for introducing recombinant DNA into a host cell?
- Genetic transformation (correct)
- Cryogenic freezing
- Microinjection
- Electrocution
What is the primary role of a cloning vector in the process of expressing a recombinant DNA molecule?
What is the primary role of a cloning vector in the process of expressing a recombinant DNA molecule?
Which of the following is a key step in the process of creating a recombinant DNA molecule?
Which of the following is a key step in the process of creating a recombinant DNA molecule?
What is the primary purpose of synthesizing target DNA in vitro in the context of recombinant DNA technology?
What is the primary purpose of synthesizing target DNA in vitro in the context of recombinant DNA technology?
What is the main purpose of using genetic markers in vectors?
What is the main purpose of using genetic markers in vectors?
Which of the following techniques is used to transfer plasmid DNA into bacteria?
Which of the following techniques is used to transfer plasmid DNA into bacteria?
What is the purpose of the 'pre-treatment' step in the heat shock transformation method?
What is the purpose of the 'pre-treatment' step in the heat shock transformation method?
Which of the following is the most difficult step in the identification of a particular DNA fragment?
Which of the following is the most difficult step in the identification of a particular DNA fragment?
What is the purpose of the 'gene gun' used in the biolistic method?
What is the purpose of the 'gene gun' used in the biolistic method?
What is the main purpose of the 'selection' step in the identification of recombinant DNA clones?
What is the main purpose of the 'selection' step in the identification of recombinant DNA clones?
Which of the following is NOT a therapeutic protein produced commercially from cloned genes?
Which of the following is NOT a therapeutic protein produced commercially from cloned genes?
What is the purpose of developing herbicide or pesticide resistant crops?
What is the purpose of developing herbicide or pesticide resistant crops?
Which of the following is NOT a potential application of recombinant DNA technology in agriculture?
Which of the following is NOT a potential application of recombinant DNA technology in agriculture?
What is the purpose of introducing an antifreeze gene from cold water fish into plants like tobacco and potato?
What is the purpose of introducing an antifreeze gene from cold water fish into plants like tobacco and potato?
Which of the following is NOT mentioned as an application of recombinant DNA technology in medicine?
Which of the following is NOT mentioned as an application of recombinant DNA technology in medicine?
What is the primary purpose of developing 'Golden rice' through genetic modification?
What is the primary purpose of developing 'Golden rice' through genetic modification?
Flashcards are hidden until you start studying
