10 Questions
Which PCR method is used to rapidly screen colonies grown on selective media to verify the presence of a desired genetic construct or to amplify a portion of the construct?
Colony PCR
Which PCR method is used to detect genes or sequences with DNA methylation after bisulfite treatment using methylated and unmethylated primers?
Methylation-specific PCR
Which PCR method is used to identify the differences in DNA sequence among individuals or populations?
PCR for DNA fingerprinting
Which company is considered the leading pharmaceutical in San Francisco?
Genentech
What is the purpose of metabolic engineering?
To produce novel materials
What is the process of 'deconstructing' a target molecule into available starting materials called?
Retrosynthesis
Which technique is used to modify the physicochemical properties, structure, and function of peptides and proteins?
Protein engineering
What is the purpose of sequence optimization in protein engineering?
To prevent proteolytic degradation
Which technique involves multiple rounds of random mutagenesis to accumulate desired beneficial mutations?
Directed evolution
Which technique is used to screen protein-protein binding interactions and enrich mutational variants?
Affinity-based evolution platforms
Study Notes
PCR Methods
- Colony PCR is used to rapidly screen colonies grown on selective media to verify the presence of a desired genetic construct or to amplify a portion of the construct.
- Methylation-specific PCR is used to detect genes or sequences with DNA methylation after bisulfite treatment using methylated and unmethylated primers.
- Restriction fragment length polymorphism (RFLP) analysis is used to identify the differences in DNA sequence among individuals or populations.
Biotech Industry
- Genentech is considered the leading pharmaceutical in San Francisco.
Metabolic Engineering
- The purpose of metabolic engineering is to design and construct new metabolic pathways or modify existing ones to produce novel compounds or improve existing ones.
Chemical Synthesis
- Retrosynthetic analysis is the process of 'deconstructing' a target molecule into available starting materials.
Protein Engineering
- Chemical modification is used to modify the physicochemical properties, structure, and function of peptides and proteins.
- The purpose of sequence optimization in protein engineering is to design and construct new protein sequences with improved functions or properties.
- Directed evolution is a technique that involves multiple rounds of random mutagenesis to accumulate desired beneficial mutations.
- Yeast two-hybrid screening is used to screen protein-protein binding interactions and enrich mutational variants.
Test your knowledge on different PCR techniques such as Multiplex PCR, Nested PCR, Colony PCR, and Loop-mediated isothermal amplification (LAMP) assay. Learn about the specific primer sets used and their applications in DNA amplification.
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