Podcast
Questions and Answers
Which PCR method is used to rapidly screen colonies grown on selective media to verify the presence of a desired genetic construct or to amplify a portion of the construct?
Which PCR method is used to rapidly screen colonies grown on selective media to verify the presence of a desired genetic construct or to amplify a portion of the construct?
- Colony PCR (correct)
- Multiplex PCR
- Loop-mediated isothermal amplification (LAMP) assay
- Nested PCR
Which PCR method is used to detect genes or sequences with DNA methylation after bisulfite treatment using methylated and unmethylated primers?
Which PCR method is used to detect genes or sequences with DNA methylation after bisulfite treatment using methylated and unmethylated primers?
- Methylation-specific PCR (correct)
- Colony PCR
- Multiplex PCR
- Loop-mediated isothermal amplification (LAMP) assay
Which PCR method is used to identify the differences in DNA sequence among individuals or populations?
Which PCR method is used to identify the differences in DNA sequence among individuals or populations?
- Loop-mediated isothermal amplification (LAMP) assay
- PCR for DNA fingerprinting (correct)
- Multiplex PCR
- Nested PCR
Which company is considered the leading pharmaceutical in San Francisco?
Which company is considered the leading pharmaceutical in San Francisco?
What is the purpose of metabolic engineering?
What is the purpose of metabolic engineering?
What is the process of 'deconstructing' a target molecule into available starting materials called?
What is the process of 'deconstructing' a target molecule into available starting materials called?
Which technique is used to modify the physicochemical properties, structure, and function of peptides and proteins?
Which technique is used to modify the physicochemical properties, structure, and function of peptides and proteins?
What is the purpose of sequence optimization in protein engineering?
What is the purpose of sequence optimization in protein engineering?
Which technique involves multiple rounds of random mutagenesis to accumulate desired beneficial mutations?
Which technique involves multiple rounds of random mutagenesis to accumulate desired beneficial mutations?
Which technique is used to screen protein-protein binding interactions and enrich mutational variants?
Which technique is used to screen protein-protein binding interactions and enrich mutational variants?
Flashcards
Colony PCR
Colony PCR
Rapidly screens colonies on selective media to verify the presence of a desired genetic construct or amplify a portion of it.
Methylation-specific PCR
Methylation-specific PCR
Detects genes or sequences with DNA methylation after bisulfite treatment using methylated and unmethylated primers.
PCR for DNA fingerprinting
PCR for DNA fingerprinting
Used to identify the differences in DNA sequence among individuals or populations.
Purpose of Metabolic Engineering
Purpose of Metabolic Engineering
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Retrosynthesis
Retrosynthesis
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Protein engineering
Protein engineering
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Sequence optimization in protein engineering
Sequence optimization in protein engineering
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Directed evolution
Directed evolution
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Affinity-based evolution platforms
Affinity-based evolution platforms
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Study Notes
PCR Methods
- Colony PCR is used to rapidly screen colonies grown on selective media to verify the presence of a desired genetic construct or to amplify a portion of the construct.
- Methylation-specific PCR is used to detect genes or sequences with DNA methylation after bisulfite treatment using methylated and unmethylated primers.
- Restriction fragment length polymorphism (RFLP) analysis is used to identify the differences in DNA sequence among individuals or populations.
Biotech Industry
- Genentech is considered the leading pharmaceutical in San Francisco.
Metabolic Engineering
- The purpose of metabolic engineering is to design and construct new metabolic pathways or modify existing ones to produce novel compounds or improve existing ones.
Chemical Synthesis
- Retrosynthetic analysis is the process of 'deconstructing' a target molecule into available starting materials.
Protein Engineering
- Chemical modification is used to modify the physicochemical properties, structure, and function of peptides and proteins.
- The purpose of sequence optimization in protein engineering is to design and construct new protein sequences with improved functions or properties.
- Directed evolution is a technique that involves multiple rounds of random mutagenesis to accumulate desired beneficial mutations.
- Yeast two-hybrid screening is used to screen protein-protein binding interactions and enrich mutational variants.
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