Podcast
Questions and Answers
Why is DNA preferred over RNA in most cases?
Why is DNA preferred over RNA in most cases?
- DNA is more stable when frozen in TE buffer (correct)
- RNA degrades rapidly due to the presence of RNAse everywhere
- DNA can be frozen directly without much degradation
- RNA has a higher half-life at 37°C
What is the purpose of treating all reagents with DEPC in RNA analysis?
What is the purpose of treating all reagents with DEPC in RNA analysis?
- Inactivate enzymes (correct)
- Promote RNAse activity
- Enhance RNA stability
- Prevent DNA degradation
In which type of microbial community would DNA analysis be more suitable?
In which type of microbial community would DNA analysis be more suitable?
- Uncultured microorganisms
- Complex microbial communities
- Mixed community
- Isolated microorganisms (correct)
What technique can be employed to identify genes conferring a specific process in microbial communities?
What technique can be employed to identify genes conferring a specific process in microbial communities?
What is the purpose of metagenomics in microbial ecology?
What is the purpose of metagenomics in microbial ecology?
Which technique is used to amplify small amounts of DNA from samples in microbial ecology?
Which technique is used to amplify small amounts of DNA from samples in microbial ecology?
What is the main disadvantage of metagenomics in microbial community analysis?
What is the main disadvantage of metagenomics in microbial community analysis?
Which step in PCR involves the separation of the DNA strands at high temperature?
Which step in PCR involves the separation of the DNA strands at high temperature?
Which technique involves loading PCR products of mixed communities on a gel with a denaturant gradient?
Which technique involves loading PCR products of mixed communities on a gel with a denaturant gradient?
What is the main disadvantage of DGGE fingerprinting technique?
What is the main disadvantage of DGGE fingerprinting technique?
Which type of RNA reveals which members of a community are metabolically active?
Which type of RNA reveals which members of a community are metabolically active?
What is the role of messenger RNA in protein synthesis?
What is the role of messenger RNA in protein synthesis?
Which technique uses labeled probes to hybridize to target nucleic acids for detection?
Which technique uses labeled probes to hybridize to target nucleic acids for detection?
In DNA Microarray, what is the main purpose of detecting functional diversity in environments?
In DNA Microarray, what is the main purpose of detecting functional diversity in environments?
What is the primary role of ribosomal RNA (rRNA) in microbial ecology studies?
What is the primary role of ribosomal RNA (rRNA) in microbial ecology studies?
Which technique converts mRNA to cDNA for further analysis?
Which technique converts mRNA to cDNA for further analysis?
What type of genes does mRNA represent?
What type of genes does mRNA represent?
What is the main disadvantage of Hybridization-Based Assays?
What is the main disadvantage of Hybridization-Based Assays?
What does Fluorescent in situ Hybridization utilize for targeting DNA or RNA?
What does Fluorescent in situ Hybridization utilize for targeting DNA or RNA?
