19 Questions
Why is DNA preferred over RNA in most cases?
DNA is more stable when frozen in TE buffer
What is the purpose of treating all reagents with DEPC in RNA analysis?
Inactivate enzymes
In which type of microbial community would DNA analysis be more suitable?
Isolated microorganisms
What technique can be employed to identify genes conferring a specific process in microbial communities?
Metabolic fingerprinting
What is the purpose of metagenomics in microbial ecology?
To analyze the gene content of a community of microorganisms
Which technique is used to amplify small amounts of DNA from samples in microbial ecology?
PCR (Polymerase Chain Reaction)
What is the main disadvantage of metagenomics in microbial community analysis?
Contamination with humic substances
Which step in PCR involves the separation of the DNA strands at high temperature?
Denaturation
Which technique involves loading PCR products of mixed communities on a gel with a denaturant gradient?
Denaturing Gradient Gel Electrophoresis (DGGE)
What is the main disadvantage of DGGE fingerprinting technique?
Highly toxic acrylamide gel
Which type of RNA reveals which members of a community are metabolically active?
Ribosomal RNA (rRNA)
What is the role of messenger RNA in protein synthesis?
Is translated into protein
Which technique uses labeled probes to hybridize to target nucleic acids for detection?
FISH
In DNA Microarray, what is the main purpose of detecting functional diversity in environments?
Detecting functional differences in microbial communities
What is the primary role of ribosomal RNA (rRNA) in microbial ecology studies?
Identifies metabolically active members
Which technique converts mRNA to cDNA for further analysis?
Community RNA Fingerprinting
What type of genes does mRNA represent?
Expressed genes
What is the main disadvantage of Hybridization-Based Assays?
Hazardous acrylamide gel
What does Fluorescent in situ Hybridization utilize for targeting DNA or RNA?
Labeled probes
Test your knowledge on PCR techniques commonly used in microbial community analysis, such as ARDRA gel and DGGE fingerprinting. Understand how PCR products of mixed communities are loaded on a gel with denaturants like urea and formamide.
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