Podcast
Questions and Answers
Why is DNA preferred over RNA in most cases?
Why is DNA preferred over RNA in most cases?
What is the purpose of treating all reagents with DEPC in RNA analysis?
What is the purpose of treating all reagents with DEPC in RNA analysis?
In which type of microbial community would DNA analysis be more suitable?
In which type of microbial community would DNA analysis be more suitable?
What technique can be employed to identify genes conferring a specific process in microbial communities?
What technique can be employed to identify genes conferring a specific process in microbial communities?
Signup and view all the answers
What is the purpose of metagenomics in microbial ecology?
What is the purpose of metagenomics in microbial ecology?
Signup and view all the answers
Which technique is used to amplify small amounts of DNA from samples in microbial ecology?
Which technique is used to amplify small amounts of DNA from samples in microbial ecology?
Signup and view all the answers
What is the main disadvantage of metagenomics in microbial community analysis?
What is the main disadvantage of metagenomics in microbial community analysis?
Signup and view all the answers
Which step in PCR involves the separation of the DNA strands at high temperature?
Which step in PCR involves the separation of the DNA strands at high temperature?
Signup and view all the answers
Which technique involves loading PCR products of mixed communities on a gel with a denaturant gradient?
Which technique involves loading PCR products of mixed communities on a gel with a denaturant gradient?
Signup and view all the answers
What is the main disadvantage of DGGE fingerprinting technique?
What is the main disadvantage of DGGE fingerprinting technique?
Signup and view all the answers
Which type of RNA reveals which members of a community are metabolically active?
Which type of RNA reveals which members of a community are metabolically active?
Signup and view all the answers
What is the role of messenger RNA in protein synthesis?
What is the role of messenger RNA in protein synthesis?
Signup and view all the answers
Which technique uses labeled probes to hybridize to target nucleic acids for detection?
Which technique uses labeled probes to hybridize to target nucleic acids for detection?
Signup and view all the answers
In DNA Microarray, what is the main purpose of detecting functional diversity in environments?
In DNA Microarray, what is the main purpose of detecting functional diversity in environments?
Signup and view all the answers
What is the primary role of ribosomal RNA (rRNA) in microbial ecology studies?
What is the primary role of ribosomal RNA (rRNA) in microbial ecology studies?
Signup and view all the answers
Which technique converts mRNA to cDNA for further analysis?
Which technique converts mRNA to cDNA for further analysis?
Signup and view all the answers
What type of genes does mRNA represent?
What type of genes does mRNA represent?
Signup and view all the answers
What is the main disadvantage of Hybridization-Based Assays?
What is the main disadvantage of Hybridization-Based Assays?
Signup and view all the answers
What does Fluorescent in situ Hybridization utilize for targeting DNA or RNA?
What does Fluorescent in situ Hybridization utilize for targeting DNA or RNA?
Signup and view all the answers
