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Questions and Answers
What is the recommended length for a primer in PCR?
What is the recommended length for a primer in PCR?
- 18-30 bp (correct)
- 10-15 bp
- 45-50 bp
- 35-40 bp
What is the optimal melting temperature (Tm) range for a primer in PCR?
What is the optimal melting temperature (Tm) range for a primer in PCR?
- 40-50°C
- 85-90°C
- 75-80°C
- 55-72°C (correct)
What is the recommended percentage GC content for a primer in PCR?
What is the recommended percentage GC content for a primer in PCR?
- 65-75%
- 50-60% (correct)
- 35-45%
- 20-30%
Study Notes
Primer Design in PCR
- The recommended length for a primer in PCR is typically between 18-24 nucleotides.
- The optimal melting temperature (Tm) range for a primer in PCR is between 55°C-65°C, with an ideal Tm around 60°C.
- The recommended percentage GC content for a primer in PCR is between 40-60%.
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Description
Test your knowledge about the most important components of a good PCR primer design and the rules for designing effective primers, including length, Tm, GC content, hairpin structure, and primer dimer formation.
