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PCR Primer Design Basics

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Questions and Answers

What is the recommended length for a primer in PCR?

18-30 bp (correct)

10-15 bp

45-50 bp

35-40 bp

What is the optimal melting temperature (Tm) range for a primer in PCR?

40-50°C

85-90°C

75-80°C

55-72°C (correct)

What is the recommended percentage GC content for a primer in PCR?

65-75%

50-60% (correct)

35-45%

20-30%

Study Notes

Primer Design in PCR

The recommended length for a primer in PCR is typically between 18-24 nucleotides.
The optimal melting temperature (Tm) range for a primer in PCR is between 55°C-65°C, with an ideal Tm around 60°C.
The recommended percentage GC content for a primer in PCR is between 40-60%.

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Description

Test your knowledge about the most important components of a good PCR primer design and the rules for designing effective primers, including length, Tm, GC content, hairpin structure, and primer dimer formation.