PCR Primer Design Basics
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Questions and Answers

What is the recommended length for a primer in PCR?

  • 18-30 bp (correct)
  • 10-15 bp
  • 45-50 bp
  • 35-40 bp
  • What is the optimal melting temperature (Tm) range for a primer in PCR?

  • 40-50°C
  • 85-90°C
  • 75-80°C
  • 55-72°C (correct)
  • What is the recommended percentage GC content for a primer in PCR?

  • 65-75%
  • 50-60% (correct)
  • 35-45%
  • 20-30%
  • Study Notes

    Primer Design in PCR

    • The recommended length for a primer in PCR is typically between 18-24 nucleotides.
    • The optimal melting temperature (Tm) range for a primer in PCR is between 55°C-65°C, with an ideal Tm around 60°C.
    • The recommended percentage GC content for a primer in PCR is between 40-60%.

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    Description

    Test your knowledge about the most important components of a good PCR primer design and the rules for designing effective primers, including length, Tm, GC content, hairpin structure, and primer dimer formation.

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