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Applications of PCR in Molecular Biology

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Questions and Answers

What is the primary mechanism of PCR that allows for the production of many copies of a specific DNA region?

Ligation
Semiconservative replication
Thermal cycling (correct)
Transcription

What is the role of DNA primers in PCR?

To act as a heat stable DNA polymerase
To initiate transcription
To facilitate DNA ligation
To bind to the target DNA sequence (correct)

What is the name of the thermostable DNA polymerase used in PCR?

Taq polymerase (correct)
Thermus aquaticus polymerase
Pfu polymerase
E. coli polymerase

What is the minimum size of the DNA fragment that can be amplified using PCR?

50-100 bp (B) Signup and view all the answers

What is the result of PCR amplification?

Thousands to millions of copies of the target DNA region (A) Signup and view all the answers

What are the two main reagents used in PCR?

DNA primers and a heat stable DNA polymerase (C) Signup and view all the answers

What is a characteristic of the Taq polymerase enzyme?

It remains active after every heating step (C) Signup and view all the answers

Which of the following is an application of PCR in molecular biology?

Cloning of gene or gene fragments (C) Signup and view all the answers

What is the primary purpose of PCR?

To amplify specific DNA sequences (D) Signup and view all the answers

What is a common use of PCR in forensic medicine?

To determine maternity or paternity (B) Signup and view all the answers

What is a result of PCR amplification?

Millions or billions of copies of the target DNA sequence (B) Signup and view all the answers

What is a potential application of PCR in agriculture?

To determine the seed purity (C) Signup and view all the answers

What is the primary function of primers in PCR?

To bind to specific DNA sequences (A) Signup and view all the answers

What is the result of each cycle of the PCR reaction?

The original DNA sample is doubled (B) Signup and view all the answers

What is the primary advantage of PCR in molecular biology?

It allows for the analysis of different nucleic acids (B) Signup and view all the answers

Which enzyme plays a crucial role in the PCR mechanism?

Taq polymerase (C) Signup and view all the answers

What is the purpose of the template DNA in PCR?

To serve as a scaffold for DNA synthesis (A) Signup and view all the answers

What is the result of amplifying a DNA sample using PCR?

The DNA sample is amplified to millions of copies (C) Signup and view all the answers

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