Applications of PCR in Molecular Biology
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Questions and Answers

What is the primary mechanism of PCR that allows for the production of many copies of a specific DNA region?

  • Ligation
  • Semiconservative replication
  • Thermal cycling (correct)
  • Transcription

    • What is the role of DNA primers in PCR?

  • To act as a heat stable DNA polymerase
  • To initiate transcription
  • To facilitate DNA ligation
  • To bind to the target DNA sequence (correct)

    • What is the name of the thermostable DNA polymerase used in PCR?

  • Taq polymerase (correct)
  • Thermus aquaticus polymerase
  • Pfu polymerase
  • E. coli polymerase

    • What is the minimum size of the DNA fragment that can be amplified using PCR?

    <p>50-100 bp</p> Signup and view all the answers

    What is the result of PCR amplification?

    <p>Thousands to millions of copies of the target DNA region</p> Signup and view all the answers

    What are the two main reagents used in PCR?

    <p>DNA primers and a heat stable DNA polymerase</p> Signup and view all the answers

    What is a characteristic of the Taq polymerase enzyme?

    <p>It remains active after every heating step</p> Signup and view all the answers

    Which of the following is an application of PCR in molecular biology?

    <p>Cloning of gene or gene fragments</p> Signup and view all the answers

    What is the primary purpose of PCR?

    <p>To amplify specific DNA sequences</p> Signup and view all the answers

    What is a common use of PCR in forensic medicine?

    <p>To determine maternity or paternity</p> Signup and view all the answers

    What is a result of PCR amplification?

    <p>Millions or billions of copies of the target DNA sequence</p> Signup and view all the answers

    What is a potential application of PCR in agriculture?

    <p>To determine the seed purity</p> Signup and view all the answers

    What is the primary function of primers in PCR?

    <p>To bind to specific DNA sequences</p> Signup and view all the answers

    What is the result of each cycle of the PCR reaction?

    <p>The original DNA sample is doubled</p> Signup and view all the answers

    What is the primary advantage of PCR in molecular biology?

    <p>It allows for the analysis of different nucleic acids</p> Signup and view all the answers

    Which enzyme plays a crucial role in the PCR mechanism?

    <p>Taq polymerase</p> Signup and view all the answers

    What is the purpose of the template DNA in PCR?

    <p>To serve as a scaffold for DNA synthesis</p> Signup and view all the answers

    What is the result of amplifying a DNA sample using PCR?

    <p>The DNA sample is amplified to millions of copies</p> Signup and view all the answers

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