PCR Fundamentals and Primer Orientation
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Questions and Answers

What advantage does using two primers provide in a PCR reaction?

  • It enables exponential growth of DNA copies. (correct)
  • It causes the reaction to slow down.
  • It allows for more specific binding to the target DNA.
  • It facilitates the copying of longer DNA sequences.
  • Why is the orientation of the primers in a PCR reaction important?

  • They must be complementary to the DNA polymerase.
  • They must bind to the same strand of DNA.
  • They allow DNA polymerase to extend them in the correct direction. (correct)
  • They should align with the length of the DNA target.
  • What is the main limitation in the length of DNA targets that can be amplified using PCR?

  • Targets must be shorter than 50 nucleotides.
  • Target length does not affect the PCR process.
  • Targets should ideally be between 100 and a few thousand nucleotides long. (correct)
  • Targets longer than 1000 nucleotides are always preferred.
  • What role does DNA polymerase play in the PCR process?

    <p>It copies DNA in a specific direction.</p> Signup and view all the answers

    How does the number of DNA copies change with each PCR cycle when using two primers?

    <p>The number of copies doubles each cycle.</p> Signup and view all the answers

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