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Questions and Answers
What is the initial step in the PCR process?
What is the purpose of raising the temperature to about 72 °C in PCR?
How does the number of DNA copies change after each cycle in PCR?
What is the temperature at which primers anneal to the DNA template in PCR?
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Study Notes
PCR Process
- The initial step in the PCR process is denaturation, where the double-stranded DNA is separated into single strands.
PCR Temperature
- Raising the temperature to about 72 °C in PCR allows the enzyme Taq polymerase to synthesize new DNA strands by adding nucleotides to the primers.
DNA Copies
- After each cycle in PCR, the number of DNA copies doubles, resulting in an exponential increase in the amount of DNA.
Primer Annealing
- Primers anneal to the DNA template in PCR at a temperature of around 50-65 °C, which is specific to the primer sequence and allows for specific binding to the target DNA region.
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Description
Test your knowledge of the three-step process of Polymerase Chain Reaction (PCR), which includes denaturation, annealing, and extension of DNA strands.
