PCR Process Steps Quiz

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What is the initial step in the PCR process?

Separation of the two strands of the DNA molecule

What is the purpose of raising the temperature to about 72 °C in PCR?

To begin adding nucleotides onto the ends of the annealed primers

How does the number of DNA copies change after each cycle in PCR?

It doubles after each cycle

What is the temperature at which primers anneal to the DNA template in PCR?

About 55 °C

Study Notes

PCR Process

  • The initial step in the PCR process is denaturation, where the double-stranded DNA is separated into single strands.

PCR Temperature

  • Raising the temperature to about 72 °C in PCR allows the enzyme Taq polymerase to synthesize new DNA strands by adding nucleotides to the primers.

DNA Copies

  • After each cycle in PCR, the number of DNA copies doubles, resulting in an exponential increase in the amount of DNA.

Primer Annealing

  • Primers anneal to the DNA template in PCR at a temperature of around 50-65 °C, which is specific to the primer sequence and allows for specific binding to the target DNA region.

Test your knowledge of the three-step process of Polymerase Chain Reaction (PCR), which includes denaturation, annealing, and extension of DNA strands.

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