PCR and DNA Sequencing
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Questions and Answers

What is the main advantage of PCR?

  • It does not require a template DNA
  • It is a super fast technique
  • It is a super sensitive technique (correct)
  • It can select any region of a target DNA

What is the requirement for DNA sequencing?

  • A template DNA, one primer, and one enzyme
  • A template DNA and two primers
  • Two primers
  • A template DNA and one primer (correct)

Why is only one primer used in DNA sequencing?

  • To prevent geometric amplification (correct)
  • To increase the amount of target DNA
  • To act as a template for DNA synthesis
  • To select any region of a target DNA

What is the role of primers in DNA synthesis?

<p>To synthesize new DNA strands (A)</p> Signup and view all the answers

How many primers are required for PCR?

<p>Two (B)</p> Signup and view all the answers

What is the flexibility of PCR?

<p>It can amplify any region of a target DNA. (C)</p> Signup and view all the answers

What is the purpose of amplifying DNA in PCR?

<p>To increase the amount of target DNA (A)</p> Signup and view all the answers

What is the requirement for geometric amplification?

<p>A template DNA and two primers (A)</p> Signup and view all the answers

What is the main advantage of using PCR for DNA synthesis?

<p>It can amplify small amounts of DNA (C)</p> Signup and view all the answers

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