Podcast
Questions and Answers
What is the direction of DNA synthesis?
What is the direction of DNA synthesis?
- 3' → 3'
- 3' → 5'
- 5' → 5'
- 5' → 3' (correct)
What is the main difference between dideoxynucleotides and normal nucleotides?
What is the main difference between dideoxynucleotides and normal nucleotides?
- Absence of a 3'-OH group (correct)
- Presence of a 5'-OH group
- Presence of a 2'-OH group
- Presence of a 3'-OH group
What is the term for the process of determining the order of nucleotides in a piece of DNA?
What is the term for the process of determining the order of nucleotides in a piece of DNA?
- DNA sequencing (correct)
- DNA synthesis
- PCR amplification
- DNA replication
Who developed the technique of Sanger sequencing?
Who developed the technique of Sanger sequencing?
What is the role of dideoxynucleotides in Sanger sequencing?
What is the role of dideoxynucleotides in Sanger sequencing?
What is the characteristic of DNA strands that underlies both DNA replication and Watson-Crick base pairing?
What is the characteristic of DNA strands that underlies both DNA replication and Watson-Crick base pairing?
What is the typical length of oligodeoxynucleotide primers in PCR?
What is the typical length of oligodeoxynucleotide primers in PCR?
What is the temperature range at which Thermus aquaticus can survive?
What is the temperature range at which Thermus aquaticus can survive?
At what temperature is the extension of primers by Taq DNA polymerase performed in PCR?
At what temperature is the extension of primers by Taq DNA polymerase performed in PCR?
Why is Taq polymerase useful in PCR?
Why is Taq polymerase useful in PCR?
How many cycles of PCR are typically performed?
How many cycles of PCR are typically performed?
What is the primary function of PCR machines?
What is the primary function of PCR machines?
What type of fossil was found in African populations in 2011?
What type of fossil was found in African populations in 2011?
What is the minimum age of the fossils found in the cave?
What is the minimum age of the fossils found in the cave?
What is the purpose of introducing mismatches in a primer during PCR?
What is the purpose of introducing mismatches in a primer during PCR?
What is the advantage of PCR in forensic science?
What is the advantage of PCR in forensic science?
What is the term for the application of PCR in legal cases?
What is the term for the application of PCR in legal cases?
What is the benefit of understanding PCR techniques in the context of human origins?
What is the benefit of understanding PCR techniques in the context of human origins?
What is the main purpose of using multiple STRs in DNA profiling?
What is the main purpose of using multiple STRs in DNA profiling?
What is the name of the system operated by the FBI for DNA profiling?
What is the name of the system operated by the FBI for DNA profiling?
How many STRs are typically analyzed in a multiplex PCR reaction?
How many STRs are typically analyzed in a multiplex PCR reaction?
What is the purpose of identifying X and Y chromosomes in a separate reaction?
What is the purpose of identifying X and Y chromosomes in a separate reaction?
What is the name of the European standard set of STRs used in DNA profiling?
What is the name of the European standard set of STRs used in DNA profiling?
What is the purpose of analyzing VNTRs in DNA profiling?
What is the purpose of analyzing VNTRs in DNA profiling?
What is the purpose of heating to 94°C in the PCR cycle?
What is the purpose of heating to 94°C in the PCR cycle?
What is the role of the primers in the PCR cycle?
What is the role of the primers in the PCR cycle?
What is the effect of repeating the PCR cycle 30 times?
What is the effect of repeating the PCR cycle 30 times?
What is the purpose of agarose gel electrophoresis in PCR?
What is the purpose of agarose gel electrophoresis in PCR?
What is the charge of DNA in the agarose gel electrophoresis?
What is the charge of DNA in the agarose gel electrophoresis?
What is the purpose of the Taq polymerase in the PCR cycle?
What is the purpose of the Taq polymerase in the PCR cycle?
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