Podcast
Play an AI-generated podcast conversation about this lesson
Questions and Answers
Which of the following is NOT a role of water in PCR reaction?
Which of the following is NOT a role of water in PCR reaction?
- Supply optimal pH and monovalent salt environment
- Provide a liquid environment for the reaction to take place
- Supply Mg++ divalent cations as a cofactor (correct)
- Serve as a matrix for interaction of other reagents
What is the purpose of PCR buffer in the reaction?
What is the purpose of PCR buffer in the reaction?
- To supply optimal pH and monovalent salt environment (correct)
- To supply Mg++ divalent cations as a cofactor
- To serve as a matrix for interaction of other reagents
- To provide a liquid environment for the reaction to take place
Which component of PCR reaction supplies the Mg++ divalent cations required as a cofactor?
Which component of PCR reaction supplies the Mg++ divalent cations required as a cofactor?
- PCR buffer
- Water
- dNTPs
- MgCl2 (correct)
What is the role of dNTPs in PCR reaction?
What is the role of dNTPs in PCR reaction?
Signup and view all the answers
Which of the following is NOT a role of MgCl2 in PCR reaction?
Which of the following is NOT a role of MgCl2 in PCR reaction?
Signup and view all the answers
