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PCR

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PCR

Test your knowledge on Polymerase Chain Reaction (PCR), a laboratory technique used to amplify target genes. Learn about its conceptualization and operationalization by Kary Mullis and colleagues in the 1980's, and its significance as an in vitro version of DNA replication. Assess your understanding of PCR reaction components and materials.

Created by

@WinningBurgundy

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Questions and Answers

Which of the following is NOT a role of water in PCR reaction?

Supply Mg++ divalent cations as a cofactor

What is the purpose of PCR buffer in the reaction?

To supply optimal pH and monovalent salt environment

Which component of PCR reaction supplies the Mg++ divalent cations required as a cofactor?

MgCl2

What is the role of dNTPs in PCR reaction?

<p>Serve as DNA bases</p> Signup and view all the answers

Which of the following is NOT a role of MgCl2 in PCR reaction?

<p>Provide a liquid environment for the reaction to take place</p> Signup and view all the answers

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