PCR
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Questions and Answers

Which of the following is NOT a role of water in PCR reaction?

  • Supply optimal pH and monovalent salt environment
  • Provide a liquid environment for the reaction to take place
  • Supply Mg++ divalent cations as a cofactor (correct)
  • Serve as a matrix for interaction of other reagents

    • What is the purpose of PCR buffer in the reaction?

  • To supply optimal pH and monovalent salt environment (correct)
  • To supply Mg++ divalent cations as a cofactor
  • To serve as a matrix for interaction of other reagents
  • To provide a liquid environment for the reaction to take place

    • Which component of PCR reaction supplies the Mg++ divalent cations required as a cofactor?

  • PCR buffer
  • Water
  • dNTPs
  • MgCl2 (correct)

    • What is the role of dNTPs in PCR reaction?

    <p>Serve as DNA bases</p> Signup and view all the answers

    Which of the following is NOT a role of MgCl2 in PCR reaction?

    <p>Provide a liquid environment for the reaction to take place</p> Signup and view all the answers

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