Podcast
Questions and Answers
What does the field of Clinical Biochemistry primarily investigate?
What does the field of Clinical Biochemistry primarily investigate?
- The organization of tissues
- The genetics of tumors
- The effects of diseases on blood cells
- The measurement of chemicals in bodily fluids (correct)
Which area of pathology is focused on blood cell production?
Which area of pathology is focused on blood cell production?
- Histopathology
- Immunology
- Microbiology
- Haematology (correct)
What does Blood Bank Transfusion Medicine analyze?
What does Blood Bank Transfusion Medicine analyze?
- Types of infections in the blood
- Chemical imbalances in the bloodstream
- Genetic material in blood cells
- Patient's blood group and appropriate transfusions (correct)
Which branch of pathology studies infections caused by microorganisms?
Which branch of pathology studies infections caused by microorganisms?
The focus of Immunology in pathology is primarily on what?
The focus of Immunology in pathology is primarily on what?
What does Histopathology primarily involve?
What does Histopathology primarily involve?
Molecular Genetics in pathology aims to detect which of the following?
Molecular Genetics in pathology aims to detect which of the following?
Why is Clinical Biochemistry considered important in medicine?
Why is Clinical Biochemistry considered important in medicine?
What is the main limitation of using high levels of analyte in sandwich assays?
What is the main limitation of using high levels of analyte in sandwich assays?
What principle does the osmometer utilize to measure osmolality?
What principle does the osmometer utilize to measure osmolality?
Why are cystic fibrosis patients notable for having higher chloride levels in their sweat?
Why are cystic fibrosis patients notable for having higher chloride levels in their sweat?
What factor is crucial for the accurate operation of the sweat chloride analyser?
What factor is crucial for the accurate operation of the sweat chloride analyser?
Which condition can be investigated using osmolality measurements?
Which condition can be investigated using osmolality measurements?
What is the consequence of the heat of fusion during freezing point depression in an osmometer?
What is the consequence of the heat of fusion during freezing point depression in an osmometer?
What process occurs when sweat is collected for chloride analysis?
What process occurs when sweat is collected for chloride analysis?
What causes xanthochromia in cerebrospinal fluid?
What causes xanthochromia in cerebrospinal fluid?
What does a high blood glucose level indicate?
What does a high blood glucose level indicate?
What is the main principle behind the Ion Selective Electrode (ISE) method?
What is the main principle behind the Ion Selective Electrode (ISE) method?
Which of the following is NOT a cause of high sodium levels?
Which of the following is NOT a cause of high sodium levels?
What is one disadvantage of the indirect ISE method?
What is one disadvantage of the indirect ISE method?
Which of the following analytes is NOT typically measured using the Roche c702 modules?
Which of the following analytes is NOT typically measured using the Roche c702 modules?
What is a significant advantage of the indirect ISE method?
What is a significant advantage of the indirect ISE method?
How long does it typically take for the Roche c702 modules to complete a test?
How long does it typically take for the Roche c702 modules to complete a test?
Which condition is likely to interfere with the accuracy of the indirect ISE results?
Which condition is likely to interfere with the accuracy of the indirect ISE results?
What effect do interferents have on sample measurements?
What effect do interferents have on sample measurements?
Which of the following is a reason for the automatic removal of a CRP result in newborns?
Which of the following is a reason for the automatic removal of a CRP result in newborns?
What is the primary purpose of serum indices in the chemistry module?
What is the primary purpose of serum indices in the chemistry module?
Which technique is utilized in the Roche Cobas e801 modules for immunoassays?
Which technique is utilized in the Roche Cobas e801 modules for immunoassays?
What characterizes a sandwich immunoassay?
What characterizes a sandwich immunoassay?
What does the electrochemiluminescence reaction require for light production?
What does the electrochemiluminescence reaction require for light production?
In a competitive immunoassay, how does signal relate to analyte concentration?
In a competitive immunoassay, how does signal relate to analyte concentration?
What is the consequence of a measurement breaching a threshold for a particular assay?
What is the consequence of a measurement breaching a threshold for a particular assay?
What does a positive result for Xanthochromia suggest?
What does a positive result for Xanthochromia suggest?
Which substance's absorbance peaks are specifically detected in a Xanthochromia scan?
Which substance's absorbance peaks are specifically detected in a Xanthochromia scan?
What is a significant limitation of the Xanthochromia test?
What is a significant limitation of the Xanthochromia test?
When can the Xanthochromia test first be considered valid after a potential hemorrhagic event?
When can the Xanthochromia test first be considered valid after a potential hemorrhagic event?
Why is it important to protect the CSF sample from light during testing?
Why is it important to protect the CSF sample from light during testing?
What training is required to perform the Xanthochromia test?
What training is required to perform the Xanthochromia test?
How might previous lumbar punctures affect the Xanthochromia test results?
How might previous lumbar punctures affect the Xanthochromia test results?
Which of the following best describes the role of the spectrophotometer in the context of Xanthochromia?
Which of the following best describes the role of the spectrophotometer in the context of Xanthochromia?
What is the primary focus of Haematology in pathology?
What is the primary focus of Haematology in pathology?
Clinical Biochemistry primarily investigates which aspect in pathology?
Clinical Biochemistry primarily investigates which aspect in pathology?
What does Blood Bank Transfusion Medicine focus on?
What does Blood Bank Transfusion Medicine focus on?
What is the main goal of Immunology in pathology?
What is the main goal of Immunology in pathology?
Which branch of pathology focuses on bacterial and viral infections?
Which branch of pathology focuses on bacterial and viral infections?
What does Histopathology primarily study?
What does Histopathology primarily study?
Molecular Genetics in pathology aims to accomplish which of the following?
Molecular Genetics in pathology aims to accomplish which of the following?
Why is understanding Clinical Biochemistry crucial in diagnosing illnesses?
Why is understanding Clinical Biochemistry crucial in diagnosing illnesses?
What is the primary outcome of the excited state of the [Ru(bpy)3]2+ in the ECL reaction?
What is the primary outcome of the excited state of the [Ru(bpy)3]2+ in the ECL reaction?
In the context of osmolality, what condition can be investigated by measuring serum and urine osmolality?
In the context of osmolality, what condition can be investigated by measuring serum and urine osmolality?
What method does the sweat chloride analyser primarily utilize to determine chloride concentration?
What method does the sweat chloride analyser primarily utilize to determine chloride concentration?
What is a significant limitation of sweat chloride analysis?
What is a significant limitation of sweat chloride analysis?
What is the primary function of the osmometer in laboratory settings?
What is the primary function of the osmometer in laboratory settings?
Which feature is characteristic of xanthochromia in cerebrospinal fluid?
Which feature is characteristic of xanthochromia in cerebrospinal fluid?
Which aspect of an assay can lead to the Hooke Effect in sandwich assays?
Which aspect of an assay can lead to the Hooke Effect in sandwich assays?
What is the source of interference in some immunoassays related to biotin?
What is the source of interference in some immunoassays related to biotin?
What indicates a positive result for Xanthochromia in a CSF sample?
What indicates a positive result for Xanthochromia in a CSF sample?
What is a key limitation when interpreting results from a Xanthochromia scan?
What is a key limitation when interpreting results from a Xanthochromia scan?
What is required before a Xanthochromia test is considered valid after a suspected hemorrhage?
What is required before a Xanthochromia test is considered valid after a suspected hemorrhage?
Why is it essential to protect the CSF sample from light during the Xanthochromia test?
Why is it essential to protect the CSF sample from light during the Xanthochromia test?
How can previous lumbar punctures impact the interpretation of Xanthochromia results?
How can previous lumbar punctures impact the interpretation of Xanthochromia results?
Which of the following is NOT a component detected in a Xanthochromia scan?
Which of the following is NOT a component detected in a Xanthochromia scan?
What is an important training requirement for conducting the Xanthochromia test?
What is an important training requirement for conducting the Xanthochromia test?
What is the expected appearance of a CSF sample that tests positive for Xanthochromia?
What is the expected appearance of a CSF sample that tests positive for Xanthochromia?
What is a primary function of serum indices in the chemistry module?
What is a primary function of serum indices in the chemistry module?
In a competitive immunoassay, how does the signal relate to the concentration of the analyte?
In a competitive immunoassay, how does the signal relate to the concentration of the analyte?
What are interferents in the context of the chemistry module?
What are interferents in the context of the chemistry module?
What is the primary distinguishing factor between sandwich and competitive immunoassays?
What is the primary distinguishing factor between sandwich and competitive immunoassays?
What occurs if the serum indices measurement breaches a threshold?
What occurs if the serum indices measurement breaches a threshold?
What role does electrochemiluminescence (ECL) technology play in the Roche Cobas e801 modules?
What role does electrochemiluminescence (ECL) technology play in the Roche Cobas e801 modules?
Which type of immunoassay is more suitable for larger analytes, such as TSH?
Which type of immunoassay is more suitable for larger analytes, such as TSH?
Which components undergo a reaction to produce light in the ECL measurement principle?
Which components undergo a reaction to produce light in the ECL measurement principle?
What is the primary purpose of monitoring glucose levels in the blood?
What is the primary purpose of monitoring glucose levels in the blood?
What technology does the Roche Cobas c702 module utilize for analyzing samples?
What technology does the Roche Cobas c702 module utilize for analyzing samples?
Which of the following conditions might lead to a high potassium level in a patient?
Which of the following conditions might lead to a high potassium level in a patient?
What is a major limitation of the indirect Ion Selective Electrode (ISE) method?
What is a major limitation of the indirect Ion Selective Electrode (ISE) method?
What analytical method is specifically employed in the Cobas ISE module?
What analytical method is specifically employed in the Cobas ISE module?
What is the function of the membrane in an Ion Selective Electrode?
What is the function of the membrane in an Ion Selective Electrode?
Which of the following situations is NOT a common cause of low potassium levels?
Which of the following situations is NOT a common cause of low potassium levels?
What process is referred to as pseudohyponatraemia in the context of indirect ISE methods?
What process is referred to as pseudohyponatraemia in the context of indirect ISE methods?
Flashcards
Pathology
Pathology
The science studying the causes and effects of diseases.
Clinical Biochemistry
Clinical Biochemistry
Measures chemicals in body fluids to diagnose and monitor illness.
Haematology
Haematology
Study of blood cells and their production.
Blood Bank Transfusion Medicine
Blood Bank Transfusion Medicine
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Immunology
Immunology
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Microbiology
Microbiology
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Histopathology (Cell Pathology)
Histopathology (Cell Pathology)
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Molecular Genetics
Molecular Genetics
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Cobas ISE Module
Cobas ISE Module
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Roche Cobas c702 Modules
Roche Cobas c702 Modules
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Roche Cobas e801 Modules
Roche Cobas e801 Modules
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Low Sodium
Low Sodium
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Low Potassium
Low Potassium
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High Sodium
High Sodium
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High Potassium
High Potassium
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Xanthochromia
Xanthochromia
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Osmolality Measurement
Osmolality Measurement
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Sweat Chloride Analyser
Sweat Chloride Analyser
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Pathology Process - Diagnosis
Pathology Process - Diagnosis
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Study Notes
Overview of Pathology
- Pathology is the science that investigates the causes and effects of diseases.
Key Branches of Pathology
- Clinical Biochemistry: Measures chemicals in blood, urine, and other body fluids; critical for diagnosing and monitoring medical conditions; performs 80 routine assays and refers samples for over 300 tests.
- Haematology: Focuses on blood cells and the tissues that produce them.
- Blood Bank Transfusion Medicine: Analyzes a patient’s blood group to provide safe blood or blood components.
- Immunology: Studies the quantity and function of immune system components.
- Microbiology: Examines infections caused by microorganisms like bacteria and viruses, including treatment methods and antibiotic measurement.
- Histopathology / Cell Pathology: Investigates cell nature and their organization into tissues.
- Molecular Genetics: Aims to detect human, tumor, bacterial, or viral genes.
Importance of Clinical Biochemistry
- Disturbances in the body's chemistry often indicate illness; understanding these can facilitate diagnosis and treatment.
- Example: Elevated blood glucose levels may indicate diabetes, related to insulin production.
Pathology Process
- Diagnosis: Classical medical processes and preventive screening.
- Aetiology: Understanding the underlying defect and potential secondary pathologies.
- Monitoring: Assessing disease severity and treatment effectiveness, evaluating organ function.
- Guided Therapy: Considering genetic susceptibility in treatment strategies.
Laboratory Analysis Techniques
- Cobas ISE Module: Utilizes Ion Selective Electrode method for electrolyte measurement; handles 1800 tests/hour; quick 30-second tests; measures sodium, potassium, and chloride.
- Roche Cobas c702 Modules: General chemistry assays (e.g., urea, creatinine) using photometric technology, processes 2000 tests/hour; measures absorbance of light at different wavelengths.
- Roche Cobas e801 Modules: Immunoassay unit that quantifies analytes using antigen-antibody reactions, employing electrochemiluminescence technology.
Electrolyte Disturbances
- Low Sodium Causes: Heart failure, head injury, medications (diuretics).
- Low Potassium Causes: Inadequate nutrition, vomiting/diarrhea, medications.
- High Sodium Causes: Inadequate water intake, diabetes insipidus.
- High Potassium Causes: Kidney dysfunction, spurious samples.
Limitations of Laboratory Techniques
- Cobas ISE Module: Indirect method can yield erroneous results due to abnormal protein/lipid levels.
- Chemistry Module: Negative interference from drugs or conditions affecting sample integrity (e.g., clots, bubbles).
- Immunoassay Module: Potential for the Hook Effect with high analyte levels; interference from biotin supplementation.
Additional Diagnostic Techniques
- Osmolality Measurement: Used to assess concentrations of dissolved particles; important for diagnosing dehydration or toxic alcohol ingestion.
- Sweat Chloride Analyser: Critical for diagnosing cystic fibrosis; measures chloride levels in sweat through electrical stimulation and titration.
- Spectrophotometer (Uvikon XL): Used to detect xanthochromia in cerebrospinal fluid indicating subarachnoid hemorrhage; measures absorbance peaks for oxyhaemoglobin and bilirubin.
Xanthochromia Test Limitations
- Interference from certain drugs; requires specialist training for accurate interpretation; sensitivity to lighting; validity after 12 hours post-event.
Overview of Pathology
- Pathology is the science that investigates the causes and effects of diseases.
Key Branches of Pathology
- Clinical Biochemistry: Measures chemicals in blood, urine, and other body fluids; critical for diagnosing and monitoring medical conditions; performs 80 routine assays and refers samples for over 300 tests.
- Haematology: Focuses on blood cells and the tissues that produce them.
- Blood Bank Transfusion Medicine: Analyzes a patient’s blood group to provide safe blood or blood components.
- Immunology: Studies the quantity and function of immune system components.
- Microbiology: Examines infections caused by microorganisms like bacteria and viruses, including treatment methods and antibiotic measurement.
- Histopathology / Cell Pathology: Investigates cell nature and their organization into tissues.
- Molecular Genetics: Aims to detect human, tumor, bacterial, or viral genes.
Importance of Clinical Biochemistry
- Disturbances in the body's chemistry often indicate illness; understanding these can facilitate diagnosis and treatment.
- Example: Elevated blood glucose levels may indicate diabetes, related to insulin production.
Pathology Process
- Diagnosis: Classical medical processes and preventive screening.
- Aetiology: Understanding the underlying defect and potential secondary pathologies.
- Monitoring: Assessing disease severity and treatment effectiveness, evaluating organ function.
- Guided Therapy: Considering genetic susceptibility in treatment strategies.
Laboratory Analysis Techniques
- Cobas ISE Module: Utilizes Ion Selective Electrode method for electrolyte measurement; handles 1800 tests/hour; quick 30-second tests; measures sodium, potassium, and chloride.
- Roche Cobas c702 Modules: General chemistry assays (e.g., urea, creatinine) using photometric technology, processes 2000 tests/hour; measures absorbance of light at different wavelengths.
- Roche Cobas e801 Modules: Immunoassay unit that quantifies analytes using antigen-antibody reactions, employing electrochemiluminescence technology.
Electrolyte Disturbances
- Low Sodium Causes: Heart failure, head injury, medications (diuretics).
- Low Potassium Causes: Inadequate nutrition, vomiting/diarrhea, medications.
- High Sodium Causes: Inadequate water intake, diabetes insipidus.
- High Potassium Causes: Kidney dysfunction, spurious samples.
Limitations of Laboratory Techniques
- Cobas ISE Module: Indirect method can yield erroneous results due to abnormal protein/lipid levels.
- Chemistry Module: Negative interference from drugs or conditions affecting sample integrity (e.g., clots, bubbles).
- Immunoassay Module: Potential for the Hook Effect with high analyte levels; interference from biotin supplementation.
Additional Diagnostic Techniques
- Osmolality Measurement: Used to assess concentrations of dissolved particles; important for diagnosing dehydration or toxic alcohol ingestion.
- Sweat Chloride Analyser: Critical for diagnosing cystic fibrosis; measures chloride levels in sweat through electrical stimulation and titration.
- Spectrophotometer (Uvikon XL): Used to detect xanthochromia in cerebrospinal fluid indicating subarachnoid hemorrhage; measures absorbance peaks for oxyhaemoglobin and bilirubin.
Xanthochromia Test Limitations
- Interference from certain drugs; requires specialist training for accurate interpretation; sensitivity to lighting; validity after 12 hours post-event.
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