Podcast
Questions and Answers
What is the main purpose of exposing the dipeptide samples to a 6NAHCl solution?
What is the main purpose of exposing the dipeptide samples to a 6NAHCl solution?
What role does spraying the paper with a 0.5% solution of ninhydrin serve in paper chromatography?
What role does spraying the paper with a 0.5% solution of ninhydrin serve in paper chromatography?
If sample 1 contains arginine and asparagine, which amino acids are in sample 3?
If sample 1 contains arginine and asparagine, which amino acids are in sample 3?
How is Rf value calculated in paper chromatography?
How is Rf value calculated in paper chromatography?
Signup and view all the answers
Which amino acid pair is associated with sample 2 based on the calculations?
Which amino acid pair is associated with sample 2 based on the calculations?
Signup and view all the answers
Why are the amino acids identified from the bacterium's dipeptide samples important?
Why are the amino acids identified from the bacterium's dipeptide samples important?
Signup and view all the answers
What is a likely outcome if the Bacteria's preferred medium lacks the identified amino acids?
What is a likely outcome if the Bacteria's preferred medium lacks the identified amino acids?
Signup and view all the answers
Which of the following options correctly identifies the components present in sample 1?
Which of the following options correctly identifies the components present in sample 1?
Signup and view all the answers
Study Notes
Paper Chromatography Results
- Results are illustrated in Figure 2, showcasing the separation of amino acids.
- Rf (retention factor) values for amino acids must be calculated using a ruler.
Purpose of Procedures
- Exposing dipeptide samples to 6NAHCl breaks down dipeptides into individual amino acids.
- Spraying the chromatography paper with a 0.5% ninhydrin solution allows color development for visualization of amino acids.
Amino Acid Composition
- Sample 1 contains Arginine and Asparagine.
- Sample 2 consists of Glutamic acid and Leucine.
- Sample 3 has Valine and Serine.
Bacterial Species Context
- Each dipeptide sample correlates to essential amino acids for specific bacterial species.
- The amino acid composition is linked to the growth medium that supports bacterial survival and reproduction.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
This quiz covers the results and calculations of paper chromatography, focusing on the determination of Rf values for different amino acids. It emphasizes the practical application of chromatography techniques and analytical skills in a laboratory setting.
