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Questions and Answers
What does the name Orthomyxovirus mean?
What does the name Orthomyxovirus mean?
- Correct mucus (correct)
- Sticky virus
- Mutating virus
- Influenza virus
How many genera are included in the Orthomyxoviridae family?
How many genera are included in the Orthomyxoviridae family?
- 3
- 5
- 7 (correct)
- 9
Match the genera to the virus associated with it:
Match the genera to the virus associated with it:
Alphainfluenzavirus = Influenza A virus Betainfluenza virus = Influenza B virus Gammainfluenzavirus = Influenza C virus Delta influenza virus = Influenza C
Which genus contains tick-borne viruses that infect livestock and humans?
Which genus contains tick-borne viruses that infect livestock and humans?
Which genus contains the infectious salmon anemia virus?
Which genus contains the infectious salmon anemia virus?
What is the genome structure of orthomyxoviruses?
What is the genome structure of orthomyxoviruses?
The genome of orthomyxoviruses is segmented
The genome of orthomyxoviruses is segmented
Which of the following statements about morphology and genome type of Orthomyxoviruses is FALSE?
Which of the following statements about morphology and genome type of Orthomyxoviruses is FALSE?
What makes influenza viruses sensitive to heat and acidic pH?
What makes influenza viruses sensitive to heat and acidic pH?
What are the two types of glycoprotein spikes for influenza A and B?
What are the two types of glycoprotein spikes for influenza A and B?
Influenza C lacks neuraminidase and has only two types of glycoprotein spikes
Influenza C lacks neuraminidase and has only two types of glycoprotein spikes
Isavirus has hemagglutinin esterase and F protein
Isavirus has hemagglutinin esterase and F protein
The lipid envelop of influenza A viruses are derived from?
The lipid envelop of influenza A viruses are derived from?
What key components are found in the envelope of the Influenza A virus?
What key components are found in the envelope of the Influenza A virus?
The M1 matrix protein lies beneath the lipid layer of the Influenza A virus
The M1 matrix protein lies beneath the lipid layer of the Influenza A virus
What distinguishes genetic shift from genetic drift in orthomyxoviruses?
What distinguishes genetic shift from genetic drift in orthomyxoviruses?
How are influenza viruses named?
How are influenza viruses named?
How many hemaglutinin and neuraminidase subtypes of influenzas viruses are there, respectively ?
How many hemaglutinin and neuraminidase subtypes of influenzas viruses are there, respectively ?
Match the transmission to the species associated with it
Match the transmission to the species associated with it
Isavirus may be transmitted in water, gills being the important route
Isavirus may be transmitted in water, gills being the important route
Which virus was first identified as the cause of equine influenza in 1956?
Which virus was first identified as the cause of equine influenza in 1956?
Equine influenza viruses are the most important viral respiratory disease of horses
Equine influenza viruses are the most important viral respiratory disease of horses
Equine influenza viruses have been reported worldwide in which animals?
Equine influenza viruses have been reported worldwide in which animals?
What is the most common pathological effect of influenza virus replication in the respiratory tract?
What is the most common pathological effect of influenza virus replication in the respiratory tract?
Equine influenza virus replicates in the URT only
Equine influenza virus replicates in the URT only
What are the common symptoms of a primary infection of equine influenza?
What are the common symptoms of a primary infection of equine influenza?
What are the symptoms associated with a secondary infection of equine influenza virus?
What are the symptoms associated with a secondary infection of equine influenza virus?
What are the clinical features of equine influenza virus?
What are the clinical features of equine influenza virus?
What is the potential clinical manifestation of a pregnant mare who has a prolonged fever?
What is the potential clinical manifestation of a pregnant mare who has a prolonged fever?
If there are no complications with equine influenza virus, the infection is self limiting and the animals who were infected remain infectious at least 5 days after clinical signs
If there are no complications with equine influenza virus, the infection is self limiting and the animals who were infected remain infectious at least 5 days after clinical signs
Which of the following horses are at high risk of infection with equine influenza virus?
Which of the following horses are at high risk of infection with equine influenza virus?
Equids are the ONLY source of equine influenza virus
Equids are the ONLY source of equine influenza virus
What are the primary diagnostic methods for equine influenza?
What are the primary diagnostic methods for equine influenza?
How can equine influenza virus be detected in chicken embryos?
How can equine influenza virus be detected in chicken embryos?
Which of the following prevention and control methods is FALSE?
Which of the following prevention and control methods is FALSE?
Why are pigs considered a "mixing vessel" for influenza viruses?
Why are pigs considered a "mixing vessel" for influenza viruses?
Pigs possess both human (SAα2,6Gal) and avian (SAα2,3Gal)
receptors
Pigs possess both human (SAα2,6Gal) and avian (SAα2,3Gal) receptors
Which swine influenza virus subtype has been historically predominant in pigs?
Which swine influenza virus subtype has been historically predominant in pigs?
What is the primary transmission route of swine influenza virus among pigs?
What is the primary transmission route of swine influenza virus among pigs?
The swine influenza virus replicates in the lower respiratory tract
The swine influenza virus replicates in the lower respiratory tract
What is the primary route of transmission for swine influenza virus?
What is the primary route of transmission for swine influenza virus?
Which of the following pathological changes is associated with swine influenza virus infection in the lungs?
Which of the following pathological changes is associated with swine influenza virus infection in the lungs?
What is the typical incubation period for swine influenza virus in pigs?
What is the typical incubation period for swine influenza virus in pigs?
What is a common clinical sign observed in pigs infected with swine influenza virus?
What is a common clinical sign observed in pigs infected with swine influenza virus?
Under which of the following conditions is swine influenza virus infection typically non-fatal in pigs?
Under which of the following conditions is swine influenza virus infection typically non-fatal in pigs?
When is the optimal time frame for collecting samples to identify swine influenza virus after the onset of clinical signs?
When is the optimal time frame for collecting samples to identify swine influenza virus after the onset of clinical signs?
Which type of sample is recommended for virus identification in diagnosing swine influenza?
Which type of sample is recommended for virus identification in diagnosing swine influenza?
Which of the following methods can be used to detect swine influenza virus?
Which of the following methods can be used to detect swine influenza virus?
What diagnostic assay detects type A influenza viruses?
What diagnostic assay detects type A influenza viruses?
If deep nasal swabs are collected from pigs suspected of having swine influenza, what is the next step in confirming the diagnosis according to the content?
If deep nasal swabs are collected from pigs suspected of having swine influenza, what is the next step in confirming the diagnosis according to the content?
What is the scientific basis for using embryonated chicken eggs to detect swine influenza virus?
What is the scientific basis for using embryonated chicken eggs to detect swine influenza virus?
What change in antibody titre between paired sera samples collected 10-21 days apart indicates a recent swine influenza virus infection using the Hemagglutination Inhibition (HI) test?
What change in antibody titre between paired sera samples collected 10-21 days apart indicates a recent swine influenza virus infection using the Hemagglutination Inhibition (HI) test?
Why are molecular methods, like RT-PCR, valuable tools in diagnosing swine influenza?
Why are molecular methods, like RT-PCR, valuable tools in diagnosing swine influenza?
Which group of pathogens are important to consider in the differential diagnosis of acute respiratory disease in pigs?
Which group of pathogens are important to consider in the differential diagnosis of acute respiratory disease in pigs?
Why is cross-protection against new subtypes a major drawback of commercially available swine influenza vaccines?
Why is cross-protection against new subtypes a major drawback of commercially available swine influenza vaccines?
What is the primary economic impact of swine influenza outbreaks on pig farming?
What is the primary economic impact of swine influenza outbreaks on pig farming?
Considering the limitations of current swine influenza vaccines, what is a crucial aspect of effective control strategies?
Considering the limitations of current swine influenza vaccines, what is a crucial aspect of effective control strategies?
Why is it important to collect paired sera samples 10-21 days apart when performing HI tests for swine influenza?
Why is it important to collect paired sera samples 10-21 days apart when performing HI tests for swine influenza?
In the context of swine influenza diagnostics, what is the primary advantage of RT-PCR over serological tests like HI?
In the context of swine influenza diagnostics, what is the primary advantage of RT-PCR over serological tests like HI?
How does the genetic diversity of swine influenza viruses impact the effectiveness of current vaccination strategies?
How does the genetic diversity of swine influenza viruses impact the effectiveness of current vaccination strategies?
What would be the MOST appropriate next step after obtaining a positive RT-PCR result for swine influenza on a pig farm?
What would be the MOST appropriate next step after obtaining a positive RT-PCR result for swine influenza on a pig farm?
In what way does the ability of swine influenza virus to cause economic losses differ from diseases where therapeutic options are available?
In what way does the ability of swine influenza virus to cause economic losses differ from diseases where therapeutic options are available?
How does the need for culling in swine influenza outbreaks DIRECTLY impact the economic viability of a pig farm?
How does the need for culling in swine influenza outbreaks DIRECTLY impact the economic viability of a pig farm?
What is the MOST important factor to consider when selecting a commercial vaccine for swine influenza in a particular region?
What is the MOST important factor to consider when selecting a commercial vaccine for swine influenza in a particular region?
What aspect of swine influenza virus makes it particularly challenging to eradicate from a pig farm once an outbreak has occurred?
What aspect of swine influenza virus makes it particularly challenging to eradicate from a pig farm once an outbreak has occurred?
When was the infection in chickens referred to as 'fowl plague' first recognized?
When was the infection in chickens referred to as 'fowl plague' first recognized?
Why are avian influenza viruses considered economically important?
Why are avian influenza viruses considered economically important?
What mortality rate defines an avian influenza virus as 'Notifiable AI (NAI)'?
What mortality rate defines an avian influenza virus as 'Notifiable AI (NAI)'?
How are avian influenza viruses categorized for international trade reasons?
How are avian influenza viruses categorized for international trade reasons?
What subtypes of influenza A virus are specifically used to define Notifiable AI (NAI)?
What subtypes of influenza A virus are specifically used to define Notifiable AI (NAI)?
What is the overarching classification that includes Highly Pathogenic Notifiable Avian Influenza (HPNAI) and Low Pathogenicity Notifiable Avian Influenza (LPNAI)?
What is the overarching classification that includes Highly Pathogenic Notifiable Avian Influenza (HPNAI) and Low Pathogenicity Notifiable Avian Influenza (LPNAI)?
Where does the influenza virus replicate in birds?
Where does the influenza virus replicate in birds?
What is the primary pathological outcome of viremia caused by avian influenza virus?
What is the primary pathological outcome of viremia caused by avian influenza virus?
Which clinical feature is highly characteristic of chickens infected with Highly Pathogenic Avian Influenza (HPAI) within the first 48 hours?
Which clinical feature is highly characteristic of chickens infected with Highly Pathogenic Avian Influenza (HPAI) within the first 48 hours?
What are the common clinical signs observed in poultry infected with Low Pathogenicity Avian Influenza (LPAI)?
What are the common clinical signs observed in poultry infected with Low Pathogenicity Avian Influenza (LPAI)?
Why is clinical diagnosis of avian influenza considered challenging?
Why is clinical diagnosis of avian influenza considered challenging?
What is the initial step in laboratory testing for avian influenza (AI) detection?
What is the initial step in laboratory testing for avian influenza (AI) detection?
After a sample tests positive for the matrix protein (M) gene via RT-PCR, what is the subsequent step in avian influenza diagnosis?
After a sample tests positive for the matrix protein (M) gene via RT-PCR, what is the subsequent step in avian influenza diagnosis?
Why is sequence analysis of the H gene performed after confirming the presence of H5 or H7 genes in avian influenza diagnosis?
Why is sequence analysis of the H gene performed after confirming the presence of H5 or H7 genes in avian influenza diagnosis?
For what specific purpose is virus isolation from cloacal swabs primarily used in the context of avian influenza diagnosis?
For what specific purpose is virus isolation from cloacal swabs primarily used in the context of avian influenza diagnosis?
Which diagnostic assays are most commonly used for avian influenza detection?
Which diagnostic assays are most commonly used for avian influenza detection?
What is the primary strategy for controlling avian influenza outbreaks?
What is the primary strategy for controlling avian influenza outbreaks?
What biosecurity measure is crucial in preventing the spread of avian influenza to poultry farms?
What biosecurity measure is crucial in preventing the spread of avian influenza to poultry farms?
Why is vaccination not widely used to control Highly Pathogenic Avian Influenza (HPAI) in many developed countries?
Why is vaccination not widely used to control Highly Pathogenic Avian Influenza (HPAI) in many developed countries?
What is the purpose of conducting sequence analysis of the H gene following a positive test for H5 or H7 genes?
What is the purpose of conducting sequence analysis of the H gene following a positive test for H5 or H7 genes?
If a diagnostic test for avian influenza is positive for H5, what is the next diagnostic step?
If a diagnostic test for avian influenza is positive for H5, what is the next diagnostic step?
Bovine influenza is caused by bovine influenza B virus
Bovine influenza is caused by bovine influenza B virus
Which of the following are clinical signs of bovine influenza?
Which of the following are clinical signs of bovine influenza?
Additional clinical signs of bovine influenza virus are coughing, sneezing and breathing difficulties
Additional clinical signs of bovine influenza virus are coughing, sneezing and breathing difficulties
Sudden or unexplained deaths in bovine with bovine influenza are quite common
Sudden or unexplained deaths in bovine with bovine influenza are quite common
Flashcards
Orthomyxovirus meaning
Orthomyxovirus meaning
Means 'correct mucus'.
Genera in Orthomyxoviridae
Genera in Orthomyxoviridae
There are 7 genera included in the Orthomyxoviridae family.
Thogotovirus
Thogotovirus
Contains tick-borne viruses that infect livestock and humans.
Isavirus
Isavirus
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Orthomyxovirus genome
Orthomyxovirus genome
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Influenza sensitivity
Influenza sensitivity
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Glycoprotein spikes (A, B)
Glycoprotein spikes (A, B)
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Isavirus proteins
Isavirus proteins
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Influenza A envelope origin
Influenza A envelope origin
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Key components in Influenza A envelope
Key components in Influenza A envelope
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Genetic shift vs. drift
Genetic shift vs. drift
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Influenza virus naming
Influenza virus naming
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Influenza subtypes (H, N)
Influenza subtypes (H, N)
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Isavirus transmission
Isavirus transmission
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First equine influenza virus
First equine influenza virus
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Equine influenza significance
Equine influenza significance
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Equine influenza hosts
Equine influenza hosts
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Influenza pathological effect
Influenza pathological effect
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Primary equine influenza symptom
Primary equine influenza symptom
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Secondary equine influenza symptoms
Secondary equine influenza symptoms
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Equine influenza features
Equine influenza features
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Pregnant mare influenza risk
Pregnant mare influenza risk
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Equine influenza diagnosis
Equine influenza diagnosis
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Pigs as 'mixing vessels'
Pigs as 'mixing vessels'
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Pig influenza receptors
Pig influenza receptors
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Predominant swine flu
Predominant swine flu
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Swine flu transmission
Swine flu transmission
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Swine Influenza Virus Transmission
Swine Influenza Virus Transmission
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Swine Flu Replication Site
Swine Flu Replication Site
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Swine Flu Incubation
Swine Flu Incubation
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Swine Flu Fever
Swine Flu Fever
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Swine Flu Sample Collection
Swine Flu Sample Collection
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Swine Flu: Onset
Swine Flu: Onset
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Swine Flu Diagnosis: HI Test
Swine Flu Diagnosis: HI Test
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Swine Flu: Pathological Features
Swine Flu: Pathological Features
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Hemagglutination Inhibition (HI) Test
Hemagglutination Inhibition (HI) Test
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RT-PCR in SIV Diagnosis
RT-PCR in SIV Diagnosis
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Differential Diagnosis of Swine Respiratory Disease
Differential Diagnosis of Swine Respiratory Disease
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Swine Influenza Vaccines
Swine Influenza Vaccines
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Economic Impact of Swine Influenza
Economic Impact of Swine Influenza
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Paired Sera
Paired Sera
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Avian Influenza
Avian Influenza
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Pathogenicity (AI)
Pathogenicity (AI)
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Notifiable Avian Influenza (NAI)
Notifiable Avian Influenza (NAI)
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Highly Pathogenic Notifiable Avian Influenza (HPNAI)
Highly Pathogenic Notifiable Avian Influenza (HPNAI)
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Low Pathogenicity Notifiable Avian Influenza (LPNAI)
Low Pathogenicity Notifiable Avian Influenza (LPNAI)
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Notifiable disease
Notifiable disease
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HPAI symptoms
HPAI symptoms
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Pathogenesis of Avian Influenza
Pathogenesis of Avian Influenza
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Clinical Features of LPAI
Clinical Features of LPAI
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Diagnosis of Avian Influenza
Diagnosis of Avian Influenza
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Control measures for Avian Influenza
Control measures for Avian Influenza
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Avian Influenza: Diagnostic Steps
Avian Influenza: Diagnostic Steps
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Control of Avian Influenza
Control of Avian Influenza
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Study Notes
- Swine influenza virus is highly contagious and spreads through aerosols.
Pathogenesis
- The virus replicates in the upper respiratory tract.
- Airway plugging, peribronchial, and perivascular mononuclear cell infiltration occurs.
- Bronchopneumonia may develop.
Symptoms
- The incubation period is short, lasting 24-72 hours.
- The disease often appears in many animals simultaneously.
- High fever (≥42°C) is a key symptom.
- Nasal discharge occurs in later stages.
- The disease is typically not fatal if animals are kept warm and stress-free.
Diagnosis
- Diagnosis is based on the sudden onset of clinical symptoms.
- Virus identification requires sample collection within 24–48 hours after the appearance of clinical signs.
- Deep nasal swabs are recommended for sample collection.
- Virus identification can be done by inoculating embryonated chicken eggs.
- A hemagglutination inhibition (HI) test is used for detection.
- Enzyme-linked immunosorbent assays (ELISA) may be available for detection of type A influenza viruses.
- Hemagglutination Inhibition (HI) test is performed in primary serological tests for detection of swine influenza virus antibodies on paired sera.
- Collection of paired sera is generally recommended 10-21 days apart.
- A four-fold or greater increase in titre between the first and second sample is suggestive of a recent infection.
- Molecular methods - particularly RT-PCR (both real time and conventional) are used.
Differential Diagnosis
- Acute respiratory disease in pigs can be caused by PRRS virus infection.
- Aujeszky’s disease virus can cause acute respiratory disease in pigs.
- Porcine circovirus type 2 can cause acute respiratory disease in pigs.
- Actinobacillus pleuropneumoniae can cause acute respiratory disease in pigs.
- Bordetella bronchiseptica can cause acute respiratory disease in pigs.
- Pasteurella multocida can cause acute respiratory disease in pigs.
- Mycoplasma hyopneumoniae can cause acute respiratory disease in pigs.
Control
- No feasible therapeutic options exist for swine influenza.
- Commercial vaccines currently available are either whole virus, inactivated and are adjuvanted.
- These vaccines have a major drawback in that they do not consistently confer cross-protection against new subtypes.
- Swine influenza viruses are economically important due to losses arising from the requirement to cull infected pigs.
Avian Influenza Virus
- Avian influenza virus can cause a devastating infection in chickens referred to as "fowl plague".
- Fowl plague was recognized in 1878 in Northern Italy.
- The virus was later isolated in 1901 but identified as avian influenza (AI) in 1955.
- Avian influenza viruses are economically important due to losses arising from the requirement to eliminate infected birds.
- Avian influenza viruses are categorized for international trade reasons as high or low pathogenicity (HPAI or LPAI).
- Notifiable AI (NAI) is an infection of poultry caused by any influenza A virus of the H5 or H7 subtype or any AI virus that causes at least 75% mortality.
- NAI viruses are divided as highly pathogenic notifiable avian influenza (HPNAI) and low pathogenicity notifiable avian influenza (LPNAI).
- Avian influenza is a notifiable disease.
Pathogenesis of Avian Influenza Virus
- Viremia leads to multifocal lymphoid and visceral organ necrosis.
- Pathogenesis includes pancreatitis, myocarditis, myositis, encephalitis, and hemorrhage.
Clinical Features of HPAI
- A highly virulent strain of AI can cause sudden death without symptoms.
- Beyond 48 hours of HPAI symptoms may be present.
Clinical Features of LPAI
- May cause considerable losses due to decreased egg production.
- Symptoms include cessation of egg production, lacrimation, diarrhea, edema of the face, head, and neck, cyanosis of combs or wattles, and torticollis.
Diagnosis of Avian Influenza
- Clinical diagnosis is difficult due to variability in clinical signs.
- RT-PCR is used in laboratory testing to detect the matrix protein (M) gene, which is highly conserved in all strains of AI viruses; a positive result indicates avian influenza.
- Specific H5 and H7 genes are tested following a positive result for the matrix protein.
- If positive for H5 or H7, sequence analysis of the H gene is performed to determine cleavage sites.
- Virus isolation from cloacal swabs is done only for obtaining material used for sequence analysis.
- Agar gel immunodiffusion, ELISA and hemagglutination inhibition assay can also be used in testing.
Control of Avian Influenza
- Surveillance, biosecurity, and depopulation are implemented when HPAI viruses are detected.
- Segregating poultry from wild birds helps control the spread.
- Vaccination has not been used to control HPAI in most developed countries.
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