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One Step Growth Experiment in Virology

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Questions and Answers

What is the purpose of performing a one-step growth experiment?

To measure the number of infectious centers in a virus culture

To study the synchronous infection of host cells by phage particles (correct)

To quantify bacteriophage using plaque counting method

To observe multiple cycles of virus growth

Who were the scientists credited with first performing the one-step growth experiment?

Watson and Crick

Emory L. Ellis and Max Delbruck (correct)

Louis Pasteur and Robert Koch

Rosalind Franklin and Maurice Wilkins

Why is it important to dilute the mixture in the one-step growth experiment?

To speed up the rate of host cell lysis

To increase the number of virus particles per cell

To decrease the burst size of the viral culture

To prevent adsorption of multiple viruses per cell (correct)

What does the burst size refer to in the context of the one-step growth experiment?

The number of new virus particles released from an infected cell Signup and view all the answers

What aspect of virus replication does the one-step growth experiment reveal?

The synchronous infection of host cells by phage particles Signup and view all the answers

How do Emory L. Ellis and Max Delbruck contribute to virology and molecular biology?

Perfecting the plaque counting method for bacteriophage quantification Signup and view all the answers

What is the purpose of infecting excess host cells with phage particles in a one-step growth experiment?

To ensure synchronous infection of the host cells Signup and view all the answers

What does the plaque counting method used in a one-step growth experiment help quantify?

Infectious centers (plaque-forming units) Signup and view all the answers

Why is it important to dilute the mixture after allowing phages to adsorb on host cells in a one-step growth experiment?

To eliminate immediate adsorption of released virus particles Signup and view all the answers

What does the burst size refer to in the context of a one-step growth experiment?

The number of host cells infected per virus particle Signup and view all the answers

What is the ratio at which excess host cells are infected with phage particles in a one-step growth experiment?

1:10 Signup and view all the answers

What Nobel Prize was awarded to Max Delbruck in 1969 for his contributions to science?

Nobel Prize in Physiology or Medicine Signup and view all the answers