Podcast
Questions and Answers
What is the purpose of performing a one-step growth experiment?
What is the purpose of performing a one-step growth experiment?
- To measure the number of infectious centers in a virus culture
- To study the synchronous infection of host cells by phage particles (correct)
- To quantify bacteriophage using plaque counting method
- To observe multiple cycles of virus growth
Who were the scientists credited with first performing the one-step growth experiment?
Who were the scientists credited with first performing the one-step growth experiment?
- Watson and Crick
- Emory L. Ellis and Max Delbruck (correct)
- Louis Pasteur and Robert Koch
- Rosalind Franklin and Maurice Wilkins
Why is it important to dilute the mixture in the one-step growth experiment?
Why is it important to dilute the mixture in the one-step growth experiment?
- To speed up the rate of host cell lysis
- To increase the number of virus particles per cell
- To decrease the burst size of the viral culture
- To prevent adsorption of multiple viruses per cell (correct)
What does the burst size refer to in the context of the one-step growth experiment?
What does the burst size refer to in the context of the one-step growth experiment?
What aspect of virus replication does the one-step growth experiment reveal?
What aspect of virus replication does the one-step growth experiment reveal?
How do Emory L. Ellis and Max Delbruck contribute to virology and molecular biology?
How do Emory L. Ellis and Max Delbruck contribute to virology and molecular biology?
What is the purpose of infecting excess host cells with phage particles in a one-step growth experiment?
What is the purpose of infecting excess host cells with phage particles in a one-step growth experiment?
What does the plaque counting method used in a one-step growth experiment help quantify?
What does the plaque counting method used in a one-step growth experiment help quantify?
Why is it important to dilute the mixture after allowing phages to adsorb on host cells in a one-step growth experiment?
Why is it important to dilute the mixture after allowing phages to adsorb on host cells in a one-step growth experiment?
What does the burst size refer to in the context of a one-step growth experiment?
What does the burst size refer to in the context of a one-step growth experiment?
What is the ratio at which excess host cells are infected with phage particles in a one-step growth experiment?
What is the ratio at which excess host cells are infected with phage particles in a one-step growth experiment?
What Nobel Prize was awarded to Max Delbruck in 1969 for his contributions to science?
What Nobel Prize was awarded to Max Delbruck in 1969 for his contributions to science?
