One Step Growth Experiment in Virology

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What is the purpose of performing a one-step growth experiment?

To study the synchronous infection of host cells by phage particles

Who were the scientists credited with first performing the one-step growth experiment?

Emory L. Ellis and Max Delbruck

Why is it important to dilute the mixture in the one-step growth experiment?

To prevent adsorption of multiple viruses per cell

What does the burst size refer to in the context of the one-step growth experiment?

<p>The number of new virus particles released from an infected cell</p> Signup and view all the answers

What aspect of virus replication does the one-step growth experiment reveal?

<p>The synchronous infection of host cells by phage particles</p> Signup and view all the answers

How do Emory L. Ellis and Max Delbruck contribute to virology and molecular biology?

<p>Perfecting the plaque counting method for bacteriophage quantification</p> Signup and view all the answers

What is the purpose of infecting excess host cells with phage particles in a one-step growth experiment?

<p>To ensure synchronous infection of the host cells</p> Signup and view all the answers

What does the plaque counting method used in a one-step growth experiment help quantify?

<p>Infectious centers (plaque-forming units)</p> Signup and view all the answers

Why is it important to dilute the mixture after allowing phages to adsorb on host cells in a one-step growth experiment?

<p>To eliminate immediate adsorption of released virus particles</p> Signup and view all the answers

What does the burst size refer to in the context of a one-step growth experiment?

<p>The number of host cells infected per virus particle</p> Signup and view all the answers

What is the ratio at which excess host cells are infected with phage particles in a one-step growth experiment?

<p>1:10</p> Signup and view all the answers

What Nobel Prize was awarded to Max Delbruck in 1969 for his contributions to science?

<p>Nobel Prize in Physiology or Medicine</p> Signup and view all the answers

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