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Questions and Answers
What are the building blocks (monomers) of nucleic acids?
What are the building blocks (monomers) of nucleic acids?
Which of the following best describes the work of Johann Miescher?
Which of the following best describes the work of Johann Miescher?
What is the relationship between base pairs and the length of DNA molecules?
What is the relationship between base pairs and the length of DNA molecules?
During nucleic acid isolation, alkaline lysis is used for what purpose?
During nucleic acid isolation, alkaline lysis is used for what purpose?
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Which statement is correct about proteins and nucleic acids, based on the context?
Which statement is correct about proteins and nucleic acids, based on the context?
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What is the primary function of DNA Polymerase 3?
What is the primary function of DNA Polymerase 3?
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Which of the following enzymes is also known as RNAseH?
Which of the following enzymes is also known as RNAseH?
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What is the role of restriction endonucleases?
What is the role of restriction endonucleases?
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In which direction is the lagging strand synthesized?
In which direction is the lagging strand synthesized?
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What is the role of Polymerase 1 in DNA replication?
What is the role of Polymerase 1 in DNA replication?
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If a DNA template strand has the sequence 3'-GATC-5', what would be the corresponding sequence on the newly synthesized strand?
If a DNA template strand has the sequence 3'-GATC-5', what would be the corresponding sequence on the newly synthesized strand?
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Which of the following enzymes is responsible for degrading nucleic acids?
Which of the following enzymes is responsible for degrading nucleic acids?
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Which statement accurately describes the leading strand?
Which statement accurately describes the leading strand?
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What is the primary function of methyltransferase enzymes?
What is the primary function of methyltransferase enzymes?
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Why is it important for a bacteria's own nucleic acid to be methylated?
Why is it important for a bacteria's own nucleic acid to be methylated?
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What is the role of restriction enzymes (endonucleases) in bacteria?
What is the role of restriction enzymes (endonucleases) in bacteria?
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Which of the following best describes the function of helicases?
Which of the following best describes the function of helicases?
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Which of the following is NOT a method of genetic information recombination in asexually reproducing organisms?
Which of the following is NOT a method of genetic information recombination in asexually reproducing organisms?
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What is the difference between 'direct' and 'indirect' transfer in the context of genetic recombination in asexually reproducing organisms, as exemplified by conjugation and transduction respectively?
What is the difference between 'direct' and 'indirect' transfer in the context of genetic recombination in asexually reproducing organisms, as exemplified by conjugation and transduction respectively?
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Which of the following modifies or deletes nucleic acids?
Which of the following modifies or deletes nucleic acids?
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Which of the following enzymes is responsible for breaking the sugar-phosphate backbone of DNA?
Which of the following enzymes is responsible for breaking the sugar-phosphate backbone of DNA?
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What is the primary function of DNA ligase?
What is the primary function of DNA ligase?
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Which of the following is NOT a characteristic of plasmids, as described in the text?
Which of the following is NOT a characteristic of plasmids, as described in the text?
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What distinguishes RNA from DNA in terms of nitrogenous bases?
What distinguishes RNA from DNA in terms of nitrogenous bases?
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What is the main role of ribosomal RNA (rRNA) within a cell?
What is the main role of ribosomal RNA (rRNA) within a cell?
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Which type of enzyme degrades DNA from free 3’ hydroxyl or 5’ phosphate ends?
Which type of enzyme degrades DNA from free 3’ hydroxyl or 5’ phosphate ends?
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What is the function of methyltransferases?
What is the function of methyltransferases?
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What is the size range of most plasmids?
What is the size range of most plasmids?
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What percentage of the total cellular RNA does ribosomal RNA (rRNA) constitute?
What percentage of the total cellular RNA does ribosomal RNA (rRNA) constitute?
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Which of the following is NOT a nitrogen base found in RNA?
Which of the following is NOT a nitrogen base found in RNA?
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How does mitochondrial DNA inheritance differ from nuclear DNA inheritance?
How does mitochondrial DNA inheritance differ from nuclear DNA inheritance?
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What charge do nucleotides carry?
What charge do nucleotides carry?
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What defines a zwitter ion?
What defines a zwitter ion?
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What enzyme is responsible for catalyzing the process of transcription of DNA into RNA?
What enzyme is responsible for catalyzing the process of transcription of DNA into RNA?
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Which structure represents the three-dimensional arrangement of amino acids in a protein?
Which structure represents the three-dimensional arrangement of amino acids in a protein?
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How does blood pH influence its overall charge?
How does blood pH influence its overall charge?
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What type of RNA is involved in translating the information from mRNA into proteins?
What type of RNA is involved in translating the information from mRNA into proteins?
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What is a karyotype used for?
What is a karyotype used for?
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Which type of transcription occurs continuously regardless of cellular conditions?
Which type of transcription occurs continuously regardless of cellular conditions?
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What classification of RNA-degrading enzymes includes enzymes that cut RNA at specific sites within the molecule?
What classification of RNA-degrading enzymes includes enzymes that cut RNA at specific sites within the molecule?
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Which structure results from the interaction of multiple polypeptide chains?
Which structure results from the interaction of multiple polypeptide chains?
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What is the phenotypic expression of an organism?
What is the phenotypic expression of an organism?
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What determines the characteristics of each amino acid?
What determines the characteristics of each amino acid?
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During which stage does DNA create an identical copy of itself?
During which stage does DNA create an identical copy of itself?
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Which of the following best describes proteins?
Which of the following best describes proteins?
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What is the typical size range of transfer RNA (tRNA) in terms of base pairs?
What is the typical size range of transfer RNA (tRNA) in terms of base pairs?
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Which of the following correctly distinguishes between proteome and genome?
Which of the following correctly distinguishes between proteome and genome?
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Flashcards
Nucleic Acids
Nucleic Acids
Macromolecules made of long chains of nucleotides; include DNA and RNA.
Base Pairs
Base Pairs
Units of the length of DNA formed by nitrogenous base pairs.
DNA
DNA
Deoxyribonucleic acid; a type of nucleic acid that carries genetic instructions.
RNA
RNA
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Mendel’s Law
Mendel’s Law
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Palindromic
Palindromic
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Helicases
Helicases
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Recombination
Recombination
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Methyltransferases
Methyltransferases
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Restriction Enzymes
Restriction Enzymes
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Conjugation
Conjugation
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Transduction
Transduction
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Transformation
Transformation
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Endonucleases
Endonucleases
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Polymerase 3
Polymerase 3
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Leading Strand
Leading Strand
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Lagging Strand
Lagging Strand
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RNAse H
RNAse H
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Restriction Endonucleases
Restriction Endonucleases
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Primer
Primer
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DNA Ligase
DNA Ligase
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Plasmids
Plasmids
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Nucleases
Nucleases
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Ribosomal RNA (rRNA)
Ribosomal RNA (rRNA)
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Types of rRNA
Types of rRNA
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Pre-rRNA
Pre-rRNA
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Mitochondrial DNA
Mitochondrial DNA
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Antibiotic-Resistant Genes
Antibiotic-Resistant Genes
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Nucleotide
Nucleotide
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Zwitter Ion
Zwitter Ion
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Blood pH
Blood pH
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Primary Structure
Primary Structure
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Secondary Structure
Secondary Structure
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Tertiary Structure
Tertiary Structure
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Quaternary Structure
Quaternary Structure
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Karyotype
Karyotype
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mRNA Transcription
mRNA Transcription
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RNA Polymerase
RNA Polymerase
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Ribosomes
Ribosomes
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Transfer RNA (tRNA)
Transfer RNA (tRNA)
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Constitutive Transcription
Constitutive Transcription
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Inducible Transcription
Inducible Transcription
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Amino Acids
Amino Acids
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Proteins
Proteins
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Study Notes
Nucleic Acids and Proteins
- Nucleic acids and proteins are macromolecules in the body.
- Proteins are a major macromolecule in the body.
- Nucleic acids include DNA and RNA.
History of Molecular Diagnosis
- 1865: Mendel's Law of Heredity described inheritance patterns.
- 1866: Johann Miescher purified DNA.
- 1866: Attempts to isolate DNA via alkaline lysis (using sodium hydroxide).
- 1949: Discovery that genes contain genetic material.
- 1953: James Watson and Francis Crick determined the DNA structure.
- 1970: DNA structure (the helical shape) characterized.
- 1977: DNA sequencing developed.
- 1985: PCR (Polymerase Chain Reaction) developed by Kary Mullis.
- 2001: Complete human genome sequence published.
- 2006: Complete human genome project version released.
Nucleic Acids
- Nucleic acids are polymers of nucleotides.
- Nucleotides consist of sugar, nitrogen base, and phosphate.
- There are two main types: DNA and RNA.
- DNA is typically double stranded.
- RNA is typically single stranded.
- Differentiate based on their structure, not just strand number.
Structure of DNA and RNA
- The sugar in DNA is deoxyribose, while it is ribose in RNA.
- Nitrogen bases in DNA: adenine, guanine, cytosine, and thymine DNA
- Nitrogen bases in RNA: adenine, guanine, cytosine, uracil
- The nitrogenous bases are specific and pair according to a pattern.
- Adenine pairs with Thymine (or Uracil in RNA), and
- Cytosine pairs with Guanine.
- Two hydrogen bonds form between A and T in DNA (or A & U in RNA),
- Three hydrogen bonds form between C and G.
- Phosphates are on the 5' end and hydroxyls are on the 3' end.
- The structure is a double helix (DNA), which protects the bases inside.
DNA Replication
- DNA replication is an enzymatic process.
- The process makes two identical strands of DNA from one original strand.
- RNA primers are needed.
- Enzymes, such as DNA polymerase and ligase, catalyze this process.
- Semiconservative replication creates new DNA molecules that each consist of one original and one new strand.
DNA Replication Enzymes
- DNA Polymerase: The key enzyme for adding nucleotides to the growing DNA strand.
- Helicase: Unwinds the DNA double helix.
- Single-stranded binding proteins: Prevent the separated strands from rejoining.
- Topoisomerase: Relieves strain on the DNA ahead of the replication fork.
- Primase: Creates RNA primers, essential for DNA polymerase to start replication.
- Ligase: Seals together the fragments on the lagging strand.
Restriction Enzymes
- Three types of restriction endonucleases exist.
- They cut DNA at specific sequences.
- They're used to degrade nucleic acids
- They are used in genetic engineering (molecular biology).
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Description
Explore the fascinating world of nucleic acids and proteins, crucial macromolecules that perform various functions in the body. This quiz covers the history of molecular diagnosis, including key discoveries from Mendel to the completion of the human genome project. Test your knowledge on the structure and function of DNA and RNA.