Nucleic Acid and Nucleotide: DNA Structure and Function
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Questions and Answers

What is the wavelength at which the loss of helical structure of DNA can be measured?

  • 220 nm
  • 260 nm (correct)
  • 280 nm
  • 300 nm
  • What is the term for the increase in absorbance of purines and pyrimidines after denaturation?

  • Hydrolysis
  • Denaturation
  • Hyperchromicity (correct)
  • Hypochromicity
  • What is the temperature at which half of the helical structure of DNA is lost?

  • The melting temperature (Tm) (correct)
  • The denaturation temperature
  • The absorbance temperature
  • The helical structure temperature
  • Why do G-C base pairs have a higher melting temperature than A-T base pairs?

    <p>Because G-C base pairs have 3 hydrogen bonds</p> Signup and view all the answers

    What is the sugar found in RNA?

    <p>Ribose</p> Signup and view all the answers

    What is the pyrimidine found in RNA in place of thymine?

    <p>Uracil</p> Signup and view all the answers

    Why does RNA not obey Chargaff's rule?

    <p>Because RNA is single-stranded</p> Signup and view all the answers

    What is the product of alkali hydrolysis of RNA?

    <p>2c, 3c-cyclic diesters</p> Signup and view all the answers

    What is the reason for the difference in melting temperature between DNAs with 35% and 50% G-C content?

    <p>The difference in the number of hydrogen bonds</p> Signup and view all the answers

    What is the characteristic of RNA that allows it to fold into a double-stranded structure at certain places?

    <p>The presence of complementary base pairs</p> Signup and view all the answers

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