10 Questions
What is the wavelength at which the loss of helical structure of DNA can be measured?
260 nm
What is the term for the increase in absorbance of purines and pyrimidines after denaturation?
Hyperchromicity
What is the temperature at which half of the helical structure of DNA is lost?
The melting temperature (Tm)
Why do G-C base pairs have a higher melting temperature than A-T base pairs?
Because G-C base pairs have 3 hydrogen bonds
What is the sugar found in RNA?
Ribose
What is the pyrimidine found in RNA in place of thymine?
Uracil
Why does RNA not obey Chargaff's rule?
Because RNA is single-stranded
What is the product of alkali hydrolysis of RNA?
2c, 3c-cyclic diesters
What is the reason for the difference in melting temperature between DNAs with 35% and 50% G-C content?
The difference in the number of hydrogen bonds
What is the characteristic of RNA that allows it to fold into a double-stranded structure at certain places?
The presence of complementary base pairs
Test your knowledge on nucleic acids, including DNA structure, function, and history of discovery. Learn about the types of nucleic acids and their significance in genetic information. Prepare for your chemistry exam with this quiz!
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