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Questions and Answers
What is the wavelength at which the loss of helical structure of DNA can be measured?
What is the wavelength at which the loss of helical structure of DNA can be measured?
- 220 nm
- 260 nm (correct)
- 280 nm
- 300 nm
What is the term for the increase in absorbance of purines and pyrimidines after denaturation?
What is the term for the increase in absorbance of purines and pyrimidines after denaturation?
- Hydrolysis
- Denaturation
- Hyperchromicity (correct)
- Hypochromicity
What is the temperature at which half of the helical structure of DNA is lost?
What is the temperature at which half of the helical structure of DNA is lost?
- The melting temperature (Tm) (correct)
- The denaturation temperature
- The absorbance temperature
- The helical structure temperature
Why do G-C base pairs have a higher melting temperature than A-T base pairs?
Why do G-C base pairs have a higher melting temperature than A-T base pairs?
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What is the sugar found in RNA?
What is the sugar found in RNA?
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What is the pyrimidine found in RNA in place of thymine?
What is the pyrimidine found in RNA in place of thymine?
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Why does RNA not obey Chargaff's rule?
Why does RNA not obey Chargaff's rule?
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What is the product of alkali hydrolysis of RNA?
What is the product of alkali hydrolysis of RNA?
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What is the reason for the difference in melting temperature between DNAs with 35% and 50% G-C content?
What is the reason for the difference in melting temperature between DNAs with 35% and 50% G-C content?
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What is the characteristic of RNA that allows it to fold into a double-stranded structure at certain places?
What is the characteristic of RNA that allows it to fold into a double-stranded structure at certain places?
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