10 Questions
What is the main function of nucleases in cellular processes?
Degrade DNA molecules by breaking phosphodiester bonds
What is the main characteristic of restriction enzymes (restriction endonucleases)?
They recognize specific base sequences and cleave internal phosphodiester bonds within DNA
Which type of nuclease catalyzes the hydrolysis of terminal nucleotides from the end of DNA or RNA molecule?
Exonucleases in the 5’ to 3’ direction
Who were awarded the Nobel Prize for Physiology or Medicine for the discovery and characterization of numerous restriction endonucleases?
Daniel Nathans, Werner Arber, and Hamilton O. Smith
In which year was the first restriction endonuclease enzyme HindII isolated?
1970
Which type of nuclease can recognize specific base sequences within DNA or RNA molecules and cleave internal phosphodiester bonds?
Endonucleases
What is the main function of restriction enzymes (restriction endonucleases)?
Cleaving DNA sequence at random sites
Who was awarded the Nobel Prize for Physiology or Medicine for the discovery and characterization of numerous restriction endonucleases?
Werner Arber
What is the main characteristic of exonucleases?
Catalyzing hydrolysis of terminal nucleotides from the end of DNA
What is the significance of the first restriction endonuclease enzyme HindII?
It was used as an essential tool in recombinant DNA technology
Test your knowledge on nucleases, enzymes that degrade DNA molecules by breaking phosphodiester bonds in a DNA strand, and their role in cellular processes such as DNA replication, repair, and recombination.
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