Nucleases in Molecular Biology Lecture
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Questions and Answers

What is the main function of nucleases in cellular processes?

  • Assist in the translation of mRNA into proteins
  • Degrade DNA molecules by breaking phosphodiester bonds (correct)
  • Facilitate the synthesis of DNA and RNA
  • Promote the formation of phosphodiester bonds in DNA

What is the main characteristic of restriction enzymes (restriction endonucleases)?

  • They catalyze hydrolysis of terminal nucleotides from the end of DNA
  • They facilitate DNA replication
  • They promote RNA processing
  • They recognize specific base sequences and cleave internal phosphodiester bonds within DNA (correct)

Which type of nuclease catalyzes the hydrolysis of terminal nucleotides from the end of DNA or RNA molecule?

  • Exonucleases in the 5’ to 3’ direction (correct)
  • Endonucleases in the 5’ to 3’ direction
  • Endonucleases in the 3’ to 5’ direction
  • Exonucleases in the 3’ to 5’ direction

Who were awarded the Nobel Prize for Physiology or Medicine for the discovery and characterization of numerous restriction endonucleases?

<p>Daniel Nathans, Werner Arber, and Hamilton O. Smith (C)</p> Signup and view all the answers

In which year was the first restriction endonuclease enzyme HindII isolated?

<p>1970 (A)</p> Signup and view all the answers

Which type of nuclease can recognize specific base sequences within DNA or RNA molecules and cleave internal phosphodiester bonds?

<p>Endonucleases (D)</p> Signup and view all the answers

What is the main function of restriction enzymes (restriction endonucleases)?

<p>Cleaving DNA sequence at random sites (A)</p> Signup and view all the answers

Who was awarded the Nobel Prize for Physiology or Medicine for the discovery and characterization of numerous restriction endonucleases?

<p>Werner Arber (A)</p> Signup and view all the answers

What is the main characteristic of exonucleases?

<p>Catalyzing hydrolysis of terminal nucleotides from the end of DNA (A)</p> Signup and view all the answers

What is the significance of the first restriction endonuclease enzyme HindII?

<p>It was used as an essential tool in recombinant DNA technology (A)</p> Signup and view all the answers

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