Non-Competitive Inhibitors and Enzyme Binding

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What role does a metal ion activator serve in enzyme catalysis?

Serve as a bridge to hold the substrate and enzyme together

Enhance enzyme specificity towards a variety of substrates

Function as a stabilizing agent in the conformation for catalytic activity (correct)

Act as the primary catalytic center

What is the general nature of enzymes?

Nucleic acids

Lipids

Proteins (correct)

Carbohydrates

How do enzymes enhance the rate of chemical reactions?

By directly participating in the chemical reaction

By lowering the activation energy required for the reaction to occur (correct)

By increasing the temperature of the reaction mixture

By changing the pH of the reaction environment

What is the specific region of an enzyme where the substrate binds called?

Active site Signup and view all the answers

What is the main function of oxidoreductase enzymes?

Facilitating electron transfer reactions Signup and view all the answers

How do enzymes catalyze physiologic reactions?

By lowering the activation energy level Signup and view all the answers

What is the purpose of ensuring that the substrate concentration is in excess in enzyme reactions?

To prevent the reaction from stopping prematurely Signup and view all the answers

Which of the following is NOT considered a cofactor for enzymes?

ATP Signup and view all the answers

What is the function of coenzymes in enzyme reactions?

Stabilize the enzyme-substrate complex Signup and view all the answers

What is the role of prosthetic groups in enzyme function?

Serve as permanent coenzymes for enzymes Signup and view all the answers

In reversible reactions, what characterizes a competitive inhibitor?

It competes with the substrate for the active site of the enzyme Signup and view all the answers

What defines absolute specificity in enzymes?

Matching with only one substrate and catalyzing one specific reaction Signup and view all the answers

What is the defining characteristic of non-competitive inhibitors in enzyme kinetics?

They do not resemble the substrate and bind to the enzyme in areas other than the active site Signup and view all the answers

How do irreversible inhibitors differ from reversible inhibitors in terms of enzyme inactivation?

Irreversible inhibitors destroy part of the enzyme involved in catalytic activity Signup and view all the answers

What effect does a non-competitive inhibitor have on enzyme activity?

Slows down the rate of the reaction without inactivating the enzyme Signup and view all the answers

Why does increasing substrate levels have no influence on the binding of a non-competitive inhibitor?

Because Km remains unchanged as substrates increase Signup and view all the answers

In irreversible inhibition, how does an inhibitor act as a 'suicide inhibitor'?

By forming covalent bonds with enzymes at the active site Signup and view all the answers

How do uncompetitive inhibitors influence enzyme activity?

By binding to the enzyme-substrate complex Signup and view all the answers