Next Generation Sanger Sequencing Quiz
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Questions and Answers

What is the main process used for sequencing the PhiX174 genome?

  • NGS sequencing
  • Dideoxynucleotide sequencing
  • PacBio sequencing
  • Sanger sequencing (correct)

How many genes are actually encoded by the Human genome?

  • 109
  • 11 (correct)
  • 6
  • 103

Which sequencing technology is known for its robustness and single-molecule preparation workflow?

  • PacBio sequencing (correct)
  • Nanopore sequencing
  • Sanger sequencing
  • Illumina sequencing

What was the significant advancement in biomedical research in 2009/10/11?

<p>NGS sequencing (D)</p> Signup and view all the answers

What is a disadvantage of DNA sequencing using DNA polymerase?

<p>Low accuracy (C)</p> Signup and view all the answers

Which sequencing technology is known for generating long reads with high accuracy?

<p>PacBio sequencing (A)</p> Signup and view all the answers

Study Notes

Genome Sequencing

  • The main process used for sequencing the PhiX174 genome is Sanger sequencing.

Human Genome

  • The Human genome encodes approximately 20,000-25,000 protein-coding genes.

Sequencing Technologies

  • PacBio sequencing is known for its robustness and single-molecule preparation workflow.
  • Oxford Nanopore sequencing is known for generating long reads with high accuracy.

Advances in Biomedical Research

  • 2009-2011 saw the significant advancement of completing the 1000 Genomes Project in biomedical research.

Limitations of DNA Sequencing

  • A disadvantage of DNA sequencing using DNA polymerase is its tendency to introduce errors during DNA replication.

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Description

Test your knowledge on Next Generation and Sanger sequencing techniques, the PhiX174 genome, human genome sequencing, and the DNA sequencing revolution. Explore the history, methods, and impact of these sequencing technologies.

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