NADH Production in Electron Transport Chain

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Questions and Answers

What is produced to carry the electrons in the pathway?

  • FADH2
  • ATP
  • ADP
  • NADH (correct)

Which molecule undergoes dehydrogenation in the pathway?

  • FADH2
  • NADH (correct)
  • ADP
  • ATP

What is the primary purpose of producing NADH in the pathway?

  • Carry electrons (correct)
  • Generate heat
  • Facilitate protein synthesis
  • Store energy

Which of the following molecules is NOT involved in carrying electrons during dehydrogenation in the pathway?

<p>ATP (B)</p>
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In the pathway, what role does NADH play with respect to electron transportation?

<p>Carrier of electrons (C)</p>
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What happens at this point in the process?

<p>Formation of succinyl CoA (B)</p>
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Which structure has not yet been reformed by E2?

<p>Disulfide bond (A)</p>
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What is the status of E2's original structure at this point?

<p>Partially reformed (D)</p>
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Which molecule is undergoing formation in the process described?

<p>Succinyl CoA (C)</p>
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What is the current state of E2's disulfide bond?

<p>Broken down (C)</p>
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Which enzyme is responsible for synthesizing citrate?

<p>Isocitrate dehydrogenase (C)</p>
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Which enzyme is named after the intermediate aconitate?

<p>Citrate synthase (A)</p>
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Which enzyme is involved in converting isocitrate to alpha-ketoglutarate?

<p>Aconitase (C)</p>
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What is the main function of malate synthase in the citric acid cycle?

<p>Converting malate to oxaloacetate (C)</p>
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Which enzyme is responsible for the conversion of succinate to fumarate in the citric acid cycle?

<p>Aconitase (C)</p>
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What is the product of the hydration of Fumarase?

<p>Malate (D)</p>
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Which enzyme oxidizes the alcohol group of malate to a keto group?

<p>Malate Dehydrogenase (C)</p>
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What is the role of NAD+ in the reaction where malate is converted to oxaloacetate?

<p>Acts as a reducing agent (D)</p>
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Which molecule is produced along with NADH when malate is oxidized to oxaloacetate?

<p>CO2 (A)</p>
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What happens to the alcohol group of malate during the oxidation process?

<p>It is converted to a carboxyl group (A)</p>
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What is the fate of fats in terms of metabolism, according to the text?

<p>Fats are burned in the fire of carbohydrates (A)</p>
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Why is it mentioned that fat cannot be converted to glucose?

<p>Due to pyruvate dehydrogenase reaction being irreversible (B)</p>
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What is the fate of Acetyl CoA if oxaloacetate is not present?

<p>Acetyl CoA cannot be converted to CO2 (D)</p>
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What is the role of CoA in fat metabolism?

<p>CoA is involved in the irreversible step to form fatty acids (B)</p>
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Why is it essential for oxaloacetate to be present for Acetyl CoA metabolism?

<p>To ensure Acetyl CoA can enter the Krebs cycle (D)</p>
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What needs to be converted in succinate to a keto group?

<p>CH2–CH2 (C)</p>
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Which carbon atom needs to be reformed in the middle?

<p>Carbon in CH2 (C)</p>
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What is the targeted group for conversion in the process described?

<p>Keto group (C)</p>
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What is the aim of reforming the carbon in the middle?

<p>To convert succinate to fumarate (B)</p>
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In succinate, what will be the result of converting CH2–CH2 to a keto group?

<p>Conversion to fumarate (B)</p>
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What is the final electron acceptor in the electron transport chain during oxidative phosphorylation?

<p>Water (A)</p>
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Which process requires oxygen as a crucial component in cellular respiration?

<p>Citric acid cycle (C)</p>
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What is the primary role of the electron transport chain in cellular respiration?

<p>Generation of ATP (A)</p>
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Which molecule serves as an intermediate carrier of electrons in the electron transport chain?

<p>FADH2 (D)</p>
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What is the essential requirement for the efficient functioning of oxidative phosphorylation in mitochondria?

<p>Availability of oxygen (A)</p>
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What is the product of the eighth reaction in the pathway?

<p>Ketone (B)</p>
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Which reaction converts 5 carbon to 4 carbon in the pathway?

<p>Third reaction (C)</p>
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In which reaction of the pathway does the conversion from an alkane to an alkene occur?

<p>Fifth reaction (C)</p>
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What happens to Acetyl CoA if oxaloacetate is absent in the pathway?

<p>It accumulates in its original form (D)</p>
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Which group is formed when alcohol is converted to a ketone in the pathway?

<p>Carboxyl group (C)</p>
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What type of reaction catalyzes the final dehydrogenation in the process described?

<p>Dehydration (B)</p>
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In the described process, which molecule acts as the catalyst for the reaction?

<p>Malate (D)</p>
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What is the overall effect of oxidation in this process?

<p>Production of NADH (A)</p>
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Which type of reaction is NOT involved in the process described?

<p>Reduction (D)</p>
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What is the specific enzyme responsible for catalyzing the final dehydrogenation reaction?

<p>Malate dehydrogenase (C)</p>
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Which process inhibits aconitase from converting citrate to aconitate?

<p>Noncompetitive inhibition by fluoroacetate (A)</p>
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What type of inhibition affects the activity of aconitase in the citric acid cycle?

<p>Noncompetitive inhibition (C)</p>
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What effect does fluoroacetate have on the function of aconitase in the citric acid cycle?

<p>Inhibits aconitase function (D)</p>
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Which compound causes noncompetitive inhibition of aconitase in the citric acid cycle?

<p>Fluoroacetate (D)</p>
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What impact does fluoroacetate have on the conversion of citrate to aconitate by aconitase?

<p>Blocks the enzyme's active site (C)</p>
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What type of functional group does citrate contain that cannot be oxidized?

<p>Tertiary alcohol group (D)</p>
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How many carboxylic groups are present in citrate that cannot be oxidized?

<p>3 (A)</p>
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In citrate, what is the oxidation status of the carboxylic groups?

<p>They cannot be oxidized (C)</p>
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What characteristic of the hydroxyl group in citrate makes it resistant to oxidation?

<p>It's a tertiary alcohol (A)</p>
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Which type of functional group in citrate plays a role in its resistance to oxidation?

<p>Hydroxyl group (D)</p>
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What is the fate of the H molecule that comes out as H+ during the reaction catalyzed by isocitrate dehydrogenase?

<p>It binds with NAD+ to produce NADH (B)</p>
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What serves as the electron carrier in the reaction catalyzed by isocitrate dehydrogenase?

<p>NAD+ (D)</p>
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Which molecule binds to the other released hydrogen atom during the reaction catalyzed by isocitrate dehydrogenase?

<p>NAD+ (C)</p>
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What is the end product of the conversion of isocitrate catalyzed by isocitrate dehydrogenase?

<p>Alpha-ketoglutarate (C)</p>
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What happens to the H- ion that is produced during the reaction catalyzed by isocitrate dehydrogenase?

<p>It binds with NAD+ to form NADH (B)</p>
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What is the most likely effect of Ca+2 on the rate-limiting enzyme isocitrate dehydrogenase?

<p>Activation (A)</p>
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Which molecule is most likely to have an inhibitory effect on isocitrate dehydrogenase?

<p>NADH (A)</p>
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What is the probable impact of the absence of ADP on the regulation of isocitrate dehydrogenase?

<p>Inhibition (D)</p>
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How does the presence of NADH likely affect the activity of isocitrate dehydrogenase?

<p>Inhibition (A)</p>
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Which factor is most likely to have a dual regulatory effect on isocitrate dehydrogenase?

<p>ADP (A)</p>
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Which step in the Krebs cycle is mentioned as the only irreversible one?

<p>Conversion of Succinyl CoA to α-Ketoglutarate (C)</p>
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What reaction in the Krebs cycle involves the conversion of Fumarate to Malate?

<p>Conversion of Malate to Oxaloacetate (B)</p>
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Which compound is formed by converting Citrate to Isocitrate in the Krebs cycle?

<p>Malate (C)</p>
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In the Krebs cycle, which reaction converts Succinate to Fumarate?

<p>Conversion of Fumarate to Malate (A)</p>
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Which compound is involved in the conversion from Citrate to Isocitrate in the Krebs cycle?

<p>Succinate (C)</p>
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Which type of inhibition is exhibited by fluoroacetate on aconitase in the citric acid cycle?

<p>Noncompetitive inhibition (B)</p>
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What impact does fluoroacetate have on the activity of aconitase in the citric acid cycle?

<p>Irreversibly inhibits the enzyme (C)</p>
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What is the specific effect of fluoroacetate on the conversion process carried out by aconitase?

<p>Alters the enzyme's active site (B)</p>
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Why does fluoroacetate inhibit aconitase through noncompetitive inhibition rather than competitive inhibition?

<p>To bind to a site other than the active site (C)</p>
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What distinguishes fluoroacetate's inhibition of aconitase from competitive inhibition?

<p>Binding to the active site (A)</p>
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Flashcards

What carries electrons?

NADH carries electrons in the pathway.

Molecule with dehydrogenation?

NADH undergoes dehydrogenation in the pathway.

Why produce NADH?

The primary purpose is to carry electrons for energy production.

Not an electron carrier?

ATP is not directly involved in carrying electrons during dehydrogenation.

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NADH's role in electron transport?

NADH transports electrons to the electron transport chain.

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What is forming now?

The bond between CoA and the succinyl group is formed.

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Structure not reformed by E2?

Disulfide bond hasn't been reformed by E2 yet.

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E2's structure status?

E2's original structure is in the process of being reformed.

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Molecule forming now?

Succinyl CoA is being formed.

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E2's disulfide bond state?

E2's disulfide bond is currently broken down.

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Enzyme for citrate synthesis?

Isocitrate dehydrogenase synthesizes citrate.

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Enzyme named after intermediate?

Aconitase is named after the intermediate aconitate.

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Isocitrate conversion enzyme?

Aconitase converts isocitrate to alpha-ketoglutarate.

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Malate to oxaloacetate enzyme?

Malate synthase converts malate to oxaloacetate.

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Succinate to fumarate enzyme?

Aconitase is responsible for conversion of succinate to fumarate.

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Fumarase hydration product?

Malate is the product of Fumarase after hydration.

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Enzyme oxidizing malate's alcohol?

Malate Dehydrogenase oxidizes the alcohol group of malate to a keto group.

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NAD+ Role

NAD+ acts as an oxidizing agent.

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Product alongside NADH?

CO2 is produced along with NADH.

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Malate's alcohol group fate?

It is converted to a carboxyl group.

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Fats metabolism fate?

Fats are burned in the fire of carbohydrates.

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Fat cannot be glucose because?

Pyruvate dehydrogenase reaction is irreversible.

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Fate with no oxaloacetate?

Acetyl CoA cannot be converted to CO2.

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CoA's role in fat metabolism?

CoA is involved in the irreversible step to forming fatty acids.

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Why must oxaloacetate be always present?

To ensure Acetyl CoA can enter the Krebs cycle.

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Needs converting in succinate?

Succinate needs to become fumarate.

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Carbon atom reformed?

Carbon in CH2.

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Target conversion group?

To Keto group.

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Reforming carbon's aim?

To convert succinate to fumarate

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Terminal of CH2 being turned to keto?

Conversion to fumarate

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