Mycology Part 1 Overview

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Questions and Answers

Which of the following are characteristics of fungi?

  • Eukaryotic (correct)
  • Susceptible to antibacterial antibiotics
  • Prokaryotic
  • Lack of chlorophyll (correct)

What are the long strands of tube-like structures in fungi called?

Hyphae

Aerial mycelia are responsible for absorbing nutrients from the medium.

False (B)

Which of the following is NOT a type of vegetative mycelia?

<p>Basidiospores (C)</p> Signup and view all the answers

Septate hyphae have frequent cross walls that occur perpendicular to the outer walls of the hyphae.

<p>True (A)</p> Signup and view all the answers

What type of hyphae has few cross walls at irregular intervals?

<p>Sparsely septate hyphae</p> Signup and view all the answers

Polymorphic fungi have both yeast and mold forms in the same culture.

<p>True (A)</p> Signup and view all the answers

What is the asexual reproductive structure of a fungal spore called?

<p>Conidia (A)</p> Signup and view all the answers

What type of conidia is formed by fragmentation of fertile hyphae?

<p>Arthroconidia</p> Signup and view all the answers

Dimorphic fungi include a mold phase and a yeast or spherule phase.

<p>True (A)</p> Signup and view all the answers

Yeast grows at 37°C with increased concentration of CO2.

<p>True (A)</p> Signup and view all the answers

Which of the following is NOT a type of asexual reproduction in fungi?

<p>Basidiospores (B)</p> Signup and view all the answers

What type of asexual reproduction is produced by phialide (vase shape structure)?

<p>Phialoconidia</p> Signup and view all the answers

Chlamydospores are produced by chlamydoconidia and are composed of terminal, intercalary, and sessile structures.

<p>True (A)</p> Signup and view all the answers

Sporangiospores are produced by a sporangium.

<p>True (A)</p> Signup and view all the answers

Which of the following is NOT required for sexual reproduction in fungi?

<p>Budding (B)</p> Signup and view all the answers

What is the term for a fungus that reproduces sexually?

<p>Teleomorph</p> Signup and view all the answers

Synanamorphs have more than one anamorph present for the same teleomorph or when a single fungus produces more than one anamorph.

<p>True (A)</p> Signup and view all the answers

What type of sexual reproduction is produced by the Phylum Ascomycota?

<p>Ascospores</p> Signup and view all the answers

Basidiospores are produced by the Phylum Basidiomycota.

<p>True (A)</p> Signup and view all the answers

Zygospores are produced by Zygomycota or Mucorales.

<p>True (A)</p> Signup and view all the answers

Colonization refers to the multiplication of an organism without causing harm to the host.

<p>True (A)</p> Signup and view all the answers

Infection refers to multiplication of an organism that brings harm and can result in injury.

<p>True (A)</p> Signup and view all the answers

Which of the following is NOT a mode of transmission for fungal infections?

<p>Contact transmission (C)</p> Signup and view all the answers

When collecting a specimen for fungal culture, what is the most important concern for fungal survival?

<p>Keeping the specimen moist</p> Signup and view all the answers

It is recommended to transport blood samples for fungal culture within 2 hours of collection at room temperature.

<p>True (A)</p> Signup and view all the answers

What is the recommended concentration for CSF samples being processed for fungal culture?

<p>Concentrated</p> Signup and view all the answers

Sterile body fluids, like synovial fluid, should be transported within 15 minutes at room temperature and refrigerated immediately.

<p>False (B)</p> Signup and view all the answers

What type of specimen should be directly plated onto media at the bedside for fungal culture?

<p>Bone marrow</p> Signup and view all the answers

When collecting a vaginal swab for fungal culture, it is recommended to transport the swab within 2 hours at room temperature.

<p>True (A)</p> Signup and view all the answers

Which of the following is a mounting medium used in direct microscopic examination of fungal specimens?

<p>KOH (C)</p> Signup and view all the answers

Calcofluor white is a fluorescent dye that can be added to KOH mounts to highlight fungal elements.

<p>True (A)</p> Signup and view all the answers

In a KOH mount with Calcofluor white, fungal elements are often seen as apple green or blue-white in color, depending on the filter used on the microscope.

<p>True (A)</p> Signup and view all the answers

Which of the following stains is used for differentiation of Nocardia from Actinomyces?

<p>Acid-fast stain (D)</p> Signup and view all the answers

The Gomori Methenamine Silver Nitrate stain is primarily used to detect Histoplasma capsulatum in bone marrow or blood.

<p>True (A)</p> Signup and view all the answers

The Giemsa stain highlights fungal elements in purple blue with a clear halo in bone marrow or blood samples and appears on a pink purple background.

<p>True (A)</p> Signup and view all the answers

Acridine orange stain requires a fluorescence microscope to view fungal elements which are displayed as green fluorescent, while epithelial cells appear orange.

<p>True (A)</p> Signup and view all the answers

Flashcards

Hyphae

Long, tube-like structures that make up fungal bodies. They can be aerial (above the surface) or vegetative (growing into the medium).

Mycelia

A mass of interwoven hyphae, forming the visible part of a mold colony. This gives the colony a fuzzy or woolly appearance.

Vegetative Mycelia

These hyphae extend into the culture medium, absorbing nutrients. They are responsible for the colony's growth and development.

Aerial Mycelia

These hyphae are responsible for the visible, fuzzy appearance of a mold colony. They produce spores for reproduction.

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Antler Hyphae

A type of hyphae with swollen tips, resembling antlers. They are often found in certain fungal species.

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Racquet Hyphae

Hyphae with a club-shaped appearance, resembling a tennis racket. They are characteristic of certain fungi.

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Spiral Hyphae

Hyphae that grow in a spiral or corkscrew shape. They are often found in certain fungal species.

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Rhizoids

Hyphae that resemble roots, anchoring the fungus to its substrate. They are involved in nutrient absorption.

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Septate Hyphae

Hyphae with frequent, perpendicular cross walls. They are often found in certain fungal species.

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Sparsely Septate Hyphae

Hyphae with few cross walls at irregular intervals. The cytoplasm is continuous throughout. Also known as coenocytic hyphae.

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Yeast

A single-celled fungal organism that typically forms smooth, creamy colonies, like bacteria. They lack aerial hyphae and reproduce by budding or fission.

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Mold

A type of fungus that forms mycelia and has a fuzzy or woolly appearance. They reproduce by forming spores.

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Polymorphic Fungi

A fungus that can exist in both yeast and mold forms depending on environmental conditions. It can switch between these forms during its life cycle.

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Hyaline Hyphae

Fungi with hyaline (non-pigmented or lightly pigmented) hyphae. They appear white or clear in culture.

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Phaeoid Hyphae

Fungi with phaeoid (darkly pigmented) hyphae. They appear dark brown or black in culture.

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Asexual Reproduction

Asexual reproduction in fungi, resulting in the formation of conidia (spores). It occurs through mitosis.

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Conidia

Asexual reproductive structures of fungal spores produced by mitosis. They are typically produced on specialized structures called conidiogenous cells.

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Arthroconidia

A type of conidia that is formed by fragmentation of fertile hyphae, rather than being produced by conidiogenous cells. They are often seen as chains or rows of spores.

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Blastoconidia

Asexual spores formed by budding or fragmentation in some fungal species.

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Pseudohyphae

Asexual structures produced by budding, resembling chains of cells, but unlike hyphae, they are not true tubes.

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Sporangiospores

Asexual spores produced inside a sporangium, a sac-like structure. They are released when the sporangium ruptures.

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Chlamydospores

Thick-walled, resistant spores formed by some fungi, often at the ends of hyphae. They can survive harsh conditions.

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Sexual Reproduction

Sexual reproduction in fungi, requiring two different hyphae that fuse to form a zygospore. It involves the joining of compatible nuclei and meiosis.

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Teleomorph

A fungus that reproduces sexually. It has a sexual cycle, producing spores through meiosis.

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Anamorph

A fungus that reproduces asexually. It does not have a sexual cycle, producing spores through mitosis.

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Ascospores

Spores produced during sexual reproduction by fungi in the Ascomycota phylum. They are contained within asci (sac-like structures).

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Basidiospores

Spores produced during sexual reproduction by fungi in the Basidiomycota phylum. They are formed on basidia (club-shaped structures).

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Zygospores

Spores produced during sexual reproduction by fungi in the Zygomycota phylum. They are formed as a result of the fusion of two compatible hyphae.

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Colonization

The multiplication of an organism within the host without causing harm.

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Infection

The multiplication of an organism within the host, causing harm and leading to injury.

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Study Notes

Mycology Part 1

  • General Characteristics:
    • Yeasts vs. Molds: Yeasts are single-celled organisms forming smooth, creamy colonies. Molds are multicellular, exhibiting a fuzzy or woolly appearance due to mycelia.
    • Mycelia are masses of hyphae
    • Hyphae are long, tubular structures.
      • Septate hyphae have cross-walls perpendicular to the outer walls
      • Sparsely septate hyphae have few cross-walls at irregular intervals.
      • Coenocytic hyphae lack cross-walls.
    • Pigmentation in hyphae:
      • Hyaline (Moniliaceous): non-pigmented or lightly pigmented.
      • Phaeoid (Dematiaceous): darkly pigmented.
    • General characteristics of fungi: Eukaryotic, chitin cell walls, ergosterol in cell membranes, obligate aerobes, reproduce asexually or sexually via spores, lack chlorophyll, lack susceptibility to antibacterial antibiotics.

Reproduction

  • Asexual Reproduction (Imperfect Fungi): Results in conidia (singular, conidium) formation following mitosis.
    • Conidia: asexual reproductive structures of a fungus.
    • Arthroconidia: conidia formed by the fragmentation of fertile hyphae.

Reproduction

  • Sexual Reproduction (Perfect Fungi): Also termed "Plus and minus mating type". Two different hyphae fuse to form a zygospore. This involves the fusion of two compatible nuclei, followed by meiosis.
    • Teleomorph: sexual reproductive state of a fungus.
    • Anamorph: asexual reproductive state of a fungus.
    • Synanamorphs: multiple anamorphs for the same teleomorph, or a single fungus producing multiple anamorphs.

Taxonomy

  • Ascomycota: Sexual spores are ascospores. Includes Microsporum, Trichophyton, and Scedosporium boydii.
  • Basidiomycota: Presence of clamp connections at hyphal septations.
  • Mucorales: Produces profuse gray or white aerial mycelia, hyaline hyphae, sparsely septate hyphae.
  • Fungi Imperfecti (Deuteromycota): No sexual mode of reproduction has yet been identified.

Fungal Culture Process

  • Specimen Collection & Transport: Collection methods vary depending on the specimen type (blood, CSF, sterile fluids, tissue biopsies, etc.) which should be carefully collected and appropriately transported to maintain viability. Sterile collection techniques are crucial.
    • Cold chain (transport in cool environments) is critical for maintaining sample integrity. Rapid processing is encouraged.

Direct Microscopic Examination

  • Crucial for rapid identification and diagnosis.
    • KOH preparation: helps to break down keratin and skin layers, facilitating visualization of fungal elements.

Permanent Mounts

  • PAS (Periodic Acid Schiff): differentiates fungi.
  • Gram Stain: all fungi are Gram positive.
  • Acid-Fast Stain: differentiate Nocardia from Actinomyces.
  • Gomori Methenamine Silver Nitrate: Stains fungi black on a green background.
  • Giemsa: detects Histoplasma capsulatum in bone marrow or blood, stains fungi purple-blue with a clear halo.
  • Acridine Orange: stains fungi green under fluorescence microscopy.

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