Questions and Answers
What type of specimens are collected for mycologic cultures?
What is used for cleaning nails before specimen collection?
70% isopropyl alcohol
For cerebrospinal fluid specimens, they must be collected and transported _____ to the lab.
immediately
Urine specimens for mycologic culture should be collected at any time of the day.
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Which broth is used for blood specimen collection in mycology?
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What should be done to CSF specimens before inoculation?
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Match the body site with the possible fungal pathogens:
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Sputum specimens should be collected late at night.
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How should nails be collected for mycologic culture?
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Study Notes
Specimen Collection and Transport in Mycology
- Nail samples require scrapings or cuttings, cleaned with 70% isopropyl alcohol prior to collection.
- Blood and bone marrow specimens utilize a lysis centrifugation system and are collected in Brain Heart Infusion (BHI) broth.
- Hair sampling targets superficial and cutaneous mycoses while skin, blood, bone marrow, cerebrospinal fluid (CSF), and respiratory specimens are essential for systemic mycoses.
- Subcutaneous specimens can consist of abscess fluid, wound exudates, and tissues, as well as urogenital and fecal materials.
Cerebrospinal Fluid Collection
- CSF must be collected aseptically by a physician and transported immediately to the laboratory.
- Concentration through centrifugation is recommended before inoculation for accurate results.
- For Cryptococcus, one drop of concentrated sample may be examined microscopically using India ink or latex agglutination.
Hair Sampling Protocol
- Use sterile forceps to pull affected hair or cut it close to the scalp with sterile scissors.
- Place hair samples directly into a sterile petri dish.
- Inoculate onto fungal medium and incubate at room temperature (22°C to 30°C); fungi may show slow growth, typically taking 5-10 days.
Abscess Fluid and Wound Exudates
- Collect specimens aseptically from undrained abscesses using sterile needles and syringes.
- Tissues should be gently minced before inoculation onto culture media.
Respiratory Specimen Collection
- Collect respiratory specimens early in the morning, using sterile containers for transport.
- Examples include sputum, transtracheal aspirates, and pleural lavage fluid.
Urogenital Specimens
- Urine samples should be first-voided morning specimens, ideally collected through clean-catch or catheterized methods.
- Centrifuge urine and inoculate the resulting sediment.
- Vaginal and cervical specimens are collected using sterile swabs and placed into transport media.
Possible Fungal Pathogens by Body Site
- Blood may contain pathogens like Histoplasma capsulatum, Candida spp, Blastomyces dermatitidis, and Coccidioides immitis.
- CSF can show Histoplasma capsulatum, Candida spp, Coccidioides immitis, and Cryptococcus neoformans.
- Hair samples may reveal fungi from the Trichophyton and Microsporum genera.
- Nail infections may be attributed to Epidermophyton and Aspergillus among others.
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Description
Explore the critical processes involved in the laboratory diagnosis of fungi, focusing on specimen collection and transport. This quiz covers essential techniques for collecting samples from nails, blood, and bone marrow, ensuring accurate testing for fungal infections.
