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Questions and Answers
Specimens for fungal infection diagnosis should be collected under aseptic conditions.
Specimens for fungal infection diagnosis should be collected under aseptic conditions.
True (A)
Specimens for fungal infection diagnosis should be collected from inactive lesions.
Specimens for fungal infection diagnosis should be collected from inactive lesions.
False (B)
KOH is used in direct examination to dissolve keratin.
KOH is used in direct examination to dissolve keratin.
True (A)
India ink is used in direct examination to identify the yeast form of Blastomyces.
India ink is used in direct examination to identify the yeast form of Blastomyces.
Body fluids for fungal culture should be centrifuged and the sediment used for culture.
Body fluids for fungal culture should be centrifuged and the sediment used for culture.
Calcofluor white fluorescent stain binds to the chitin component of the fungal cell walls in direct examination
Calcofluor white fluorescent stain binds to the chitin component of the fungal cell walls in direct examination
DMSO is added to KOH to hasten the clearing in skin scrapings and nail clippings in direct examination
DMSO is added to KOH to hasten the clearing in skin scrapings and nail clippings in direct examination
Specimens for fungal infection diagnosis should be collected from inactive lesions
Specimens for fungal infection diagnosis should be collected from inactive lesions
India ink is used in direct examination to identify the yeast form of Aspergillus
India ink is used in direct examination to identify the yeast form of Aspergillus
Urine specimens for fungal culture should be used without centrifugation
Urine specimens for fungal culture should be used without centrifugation
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Study Notes
Fungal Infection Diagnosis
- Specimens for fungal infection diagnosis should be collected under aseptic conditions and from inactive lesions.
Direct Examination
- KOH is used to dissolve keratin in direct examination.
- DMSO is added to KOH to hasten the clearing in skin scrapings and nail clippings.
- Calcofluor white fluorescent stain binds to the chitin component of the fungal cell walls.
Identification of Fungal Forms
- India ink is used to identify the yeast form of Blastomyces in direct examination.
- India ink is used to identify the yeast form of Aspergillus in direct examination.
Fungal Culture
- Body fluids for fungal culture should be centrifuged and the sediment used for culture.
- Urine specimens for fungal culture should be used without centrifugation.
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