Test your knowledge about the laboratory diagnosis of fungal infections, including specimen collection and transport methods. Learn about the importance of collecting specimens from active lesions and under aseptic conditions.

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Questions and Answers

Specimens for fungal infection diagnosis should be collected under aseptic conditions.

True

Specimens for fungal infection diagnosis should be collected from inactive lesions.

False

KOH is used in direct examination to dissolve keratin.

True

India ink is used in direct examination to identify the yeast form of Blastomyces.

False Signup and view all the answers

Body fluids for fungal culture should be centrifuged and the sediment used for culture.

True Signup and view all the answers

Calcofluor white fluorescent stain binds to the chitin component of the fungal cell walls in direct examination

True Signup and view all the answers

DMSO is added to KOH to hasten the clearing in skin scrapings and nail clippings in direct examination

True Signup and view all the answers

Specimens for fungal infection diagnosis should be collected from inactive lesions

False Signup and view all the answers

India ink is used in direct examination to identify the yeast form of Aspergillus

False Signup and view all the answers

Urine specimens for fungal culture should be used without centrifugation

False Signup and view all the answers

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Study Notes

Fungal Infection Diagnosis

Specimens for fungal infection diagnosis should be collected under aseptic conditions and from inactive lesions.

Direct Examination

KOH is used to dissolve keratin in direct examination.
DMSO is added to KOH to hasten the clearing in skin scrapings and nail clippings.
Calcofluor white fluorescent stain binds to the chitin component of the fungal cell walls.

Identification of Fungal Forms

India ink is used to identify the yeast form of Blastomyces in direct examination.
India ink is used to identify the yeast form of Aspergillus in direct examination.

Fungal Culture

Body fluids for fungal culture should be centrifuged and the sediment used for culture.
Urine specimens for fungal culture should be used without centrifugation.