Muscle Physiology and Motor Proteins Quiz

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Questions and Answers

What is the primary role of motor proteins in relation to the cytoskeleton?

  • To synthesize proteins
  • To support cell shape
  • To facilitate cellular movement (correct)
  • To generate energy

Kinesin and dynein are both types of motor proteins that transport materials along microtubules.

True (A)

What are the two main types of protein structures that make up the cytoskeleton?

Microtubules and microfilaments

Myosin interacts with ATP and exists mostly as a ______.

<p>dimer</p> Signup and view all the answers

Match the types of motor proteins to their primary function:

<p>Kinesin = Transport cargo towards the positive end of microtubules Dynein = Transport cargo towards the negative end of microtubules Myosin = Facilitates muscle contraction and movement Myofilament = Support and structure in muscle cells</p> Signup and view all the answers

What is the primary purpose of T-tubules in muscle fibers?

<p>Conduct action potentials into the muscle fiber (C)</p> Signup and view all the answers

The sliding filament theory was proposed by Hugh Huxley in 1954.

<p>True (A)</p> Signup and view all the answers

What are the three components of the troponin complex?

<p>TnI, TnC, TnT</p> Signup and view all the answers

The _______ is the basic functional unit of a muscle fiber.

<p>sarcomere</p> Signup and view all the answers

Match the following muscle contraction proteins with their roles:

<p>Actin = Thin filament Myosin = Thick filament Troponin = Regulatory protein involved in contraction Tropomyosin = Covers actin binding sites</p> Signup and view all the answers

What type of muscle tissue is under voluntary control?

<p>Skeletal Muscle Tissue (C)</p> Signup and view all the answers

Smooth muscle tissue contracts involuntarily.

<p>True (A)</p> Signup and view all the answers

What structure attaches muscles to bones?

<p>Tendon</p> Signup and view all the answers

Muscle fibers are wrapped in __________.

<p>perimysium</p> Signup and view all the answers

Match the muscle types with their characteristics:

<p>Skeletal Muscle = Voluntary control Smooth Muscle = Involuntary control Cardiac Muscle = Heart contractions Striated Muscle = Striped appearance</p> Signup and view all the answers

What muscle type is responsible for the contractions of the heart?

<p>Cardiac Muscle (C)</p> Signup and view all the answers

All muscle tissues contain a single nucleus.

<p>False (B)</p> Signup and view all the answers

What is the role of actin and myosin in muscle contraction?

<p>They interact to cause muscle contraction.</p> Signup and view all the answers

What is the primary functional unit of muscle contraction?

<p>Sarcomere (D)</p> Signup and view all the answers

The I band corresponds with the region where myosin is found.

<p>False (B)</p> Signup and view all the answers

Name the protein that stabilizes actin filaments.

<p>Nebulin</p> Signup and view all the answers

The thick filament in a sarcomere is primarily made of _____ molecules.

<p>myosin</p> Signup and view all the answers

Which protein stops the depolymerization of actin filaments?

<p>Tropomodulin (A)</p> Signup and view all the answers

Match the following components of muscle contraction with their functions:

<p>Myosin = Main component of thick filaments Actin = Main component of thin filaments Nebulin = Stabilizes actin filaments Tropomodulin = Prevents actin depolymerization</p> Signup and view all the answers

Describe how the sarcomere is shortened during contraction.

<p>The myosin heads pull actin filaments toward the center, decreasing the distance between Z lines.</p> Signup and view all the answers

In a sarcomere, each thick filament is surrounded by six thin filaments.

<p>True (A)</p> Signup and view all the answers

What is the role of tropomyosin in muscle contraction?

<p>It blocks binding sites on actin (C)</p> Signup and view all the answers

Troponin and tropomyosin are involved in exposing the active sites on actin during muscle contraction.

<p>True (A)</p> Signup and view all the answers

What is needed for the myosin head group to detach from the actin filament?

<p>ATP</p> Signup and view all the answers

The _____ ion binds to troponin to initiate muscle contraction.

<p>calcium</p> Signup and view all the answers

Match the following terms with their corresponding roles:

<p>Tropomyosin = Blocks binding sites on actin Troponin = Regulates tropomyosin movement Myosin = Binds to actin during contraction ATP = Provides energy for muscle relaxation</p> Signup and view all the answers

What happens to tropomyosin when calcium ions bind to troponin?

<p>It moves away from the active sites on actin (A)</p> Signup and view all the answers

Without ATP, myosin heads will detach from the actin filaments.

<p>False (B)</p> Signup and view all the answers

What occurs during the cross-bridge attachment phase?

<p>Myosin binds to exposed active sites on actin.</p> Signup and view all the answers

What happens during the pivoting step of muscle contraction?

<p>Myosin head releases ADP and a phosphate group (A)</p> Signup and view all the answers

Myosin is permanently bound to actin until ATP is released.

<p>False (B)</p> Signup and view all the answers

What is released when myosin splits ATP?

<p>ADP and a phosphate group</p> Signup and view all the answers

The __________ step involves the myosin head pivoting toward the center of the sarcomere.

<p>pivoting</p> Signup and view all the answers

Match the following steps of muscle contraction with their descriptions:

<p>Pivoting = Myosin head moves toward the center and releases ADP Cross-bridge Detachment = ATP binds to myosin allowing it to detach from actin Myosin Reactivation = Myosin splits ATP to regain energy Calcium Regulation = Controls muscle contraction at the fiber level</p> Signup and view all the answers

What is required for myosin reactivation?

<p>ATP (D)</p> Signup and view all the answers

The force of contraction decreases as calcium concentration increases.

<p>False (B)</p> Signup and view all the answers

What role does ATP play in muscle contraction?

<p>It provides energy for myosin to pivot and detach.</p> Signup and view all the answers

Flashcards

Cytoskeleton

Internal framework of a cell, made of microtubules and microfilaments, providing structural support, enabling movement, and transporting materials.

Motor Proteins

Proteins that use energy from ATP to move along cytoskeletal filaments, like microtubules and microfilaments, carrying cargo and contributing to cellular movement.

Kinesin

A type of motor protein that moves cargo towards the plus end of microtubules, usually towards the cell's periphery.

Dynein

A type of motor protein that moves cargo towards the minus end of microtubules, usually towards the cell's center.

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Myosin

A type of motor protein most commonly associated with muscle contraction, but also present in other cells. It interacts with actin filaments.

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Skeletal Muscle

A type of muscle tissue that is responsible for voluntary movement and is characterized by a striated appearance under a microscope.

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Smooth Muscle

Involuntary muscle tissue found in the walls of organs, such as the stomach and intestines.

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Cardiac Muscle

Specialized muscle tissue found only in the heart. It is responsible for pumping blood throughout the body.

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Sarcomere

The basic functional unit of a muscle fiber, responsible for muscle contraction.

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Actin

A protein filament found in muscle fibers that interacts with myosin to generate muscle contraction.

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Muscle Contraction

The process by which muscle fibers shorten, generating force.

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Tendon

A tough, fibrous tissue that connects muscle to bone.

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What is a sarcomere?

A functional unit of muscle contraction, made of repeating units of myosin and actin filaments.

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What is the myosin filament?

A thick filament composed primarily of myosin protein that plays a crucial role in muscle contraction.

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What is the A band?

The region within a sarcomere that contains only myosin filaments, appearing dark under a microscope.

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What is the I band?

The region within a sarcomere containing only thin (actin) filaments, appearing lighter under a microscope.

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What is tropomyosin?

A protein involved in muscle contraction that binds to actin filaments, blocking the myosin binding sites and inhibiting muscle contraction.

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What is troponin?

A protein involved in muscle contraction that binds calcium and regulates the position of tropomyosin, allowing contraction to occur.

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What is tropomodulin?

A protein that caps the ends of actin filaments, preventing further polymerization.

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How does a sarcomere shorten?

The process by which a sarcomere shortens, leading to muscle contraction.

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Sarcoplasmic Reticulum

A network of interconnected tubes within muscle fibers, responsible for storing and releasing calcium ions (Ca2+), which are crucial for muscle contraction.

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T-tubules

Tiny, tube-like structures that carry action potentials (electrical signals) deep into the muscle fiber, ensuring that the signal reaches all parts of the muscle cell.

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Sliding Filament Theory

A theory that explains how muscles contract, stating that thin filaments (actin) slide past thick filaments (myosin) due to the interaction of cross-bridges, bringing Z-lines closer together and shortening the sarcomere.

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Troponin-Tropomyosin Complex

A protein complex that regulates muscle contraction. It binds to actin filaments, blocking the binding sites for myosin. When calcium ions are released, troponin binds calcium, causing a shift in tropomyosin, exposing the binding sites and allowing myosin to bind and initiate contraction.

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Myosin Head Pivoting

The myosin head, attached to actin, rotates towards the center of the sarcomere, pulling the actin filament along. This process requires energy from ATP.

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Myosin Detachment

The myosin head remains attached to the actin filament until ATP binds to it, causing the myosin to detach.

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Myosin Reactivation

After detaching from the actin filament, the myosin head gets reactivated when ATP splits into ADP and phosphate, storing energy for the next binding event.

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Calcium and Muscle Tension

The amount of muscle tension is determined by the amount of calcium ions available in the muscle fiber. Higher calcium levels lead to more force.

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Muscle Tension Regulation

The process of increasing muscle tension or force.

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Regulating Muscle Tension

The amount of calcium ions available in the muscle fiber determines the amount of force it can produce. This is controlled by various regulatory processes within the muscle fiber itself.

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What does tropomyosin do when a muscle is at rest?

The protein that blocks the active sites on the actin filament when the muscle is at rest, preventing myosin from binding.

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What is the role of troponin in muscle contraction?

The protein found in muscle fibers that binds to calcium ions, triggering a conformational change that moves tropomyosin away from the active sites on actin, allowing muscle contraction to begin.

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Explain the first step of muscle contraction: Active Site Exposure.

The process where calcium ions bind to troponin, causing troponin to move tropomyosin away from the active sites on actin, allowing myosin to bind and initiate muscle contraction.

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What happens during the second step of muscle contraction: Cross-Bridge Attachment?

The process where the myosin head group binds to an exposed active site on the actin filament, forming a cross-bridge. This binding requires the presence of ATP.

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What is the role of ATP in muscle contraction?

The energy source that is required for the myosin head group to detach from the actin filament after a power stroke.

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Explain the third step of muscle contraction: Cross-Bridge Detachment.

The process where the myosin head group detaches from the actin filament after a power stroke. This process requires ATP.

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What happens during the fourth step of muscle contraction: Myosin Head Readies for Another Cycle?

The process where the myosin head group swings back to its original position, ready to bind to another active site on the actin filament. This process also requires ATP.

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How do the four steps of muscle contraction result in muscle fiber shortening?

The repeated cycles of cross-bridge attachment, power stroke, and detachment, driven by ATP, which cause the actin filament to slide past the myosin filament, leading to muscle fiber shortening and contraction.

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Study Notes

Motor Proteins

  • Motor proteins are involved in movement within cells.
  • Microtubules and microfilaments are structures in cells made up of proteins (actin and tubulin).
  • Organisms move in three ways:
    • reorganizing cytoskeleton
    • carrying things around cells along microtubules with motor proteins
    • pulling on elements of cytoskeleton by motor proteins

Motor Protein Kinesin

  • Kinesin is a motor protein that moves vesicles along microtubules.
  • Kinesin carries cargo to the microtubule-organizing center (MTOC).
  • Kinesin operates along microtubules using ATP (adenosine triphosphate) as fuel.

Motor Protein Dynein

  • Dynein is another motor protein.
  • Dynein carries cargo back to the microtubule organizing center (MTOC).
  • Dynein operates along microtubules using ATP.

Structure of Kinesin

  • Kinesin has a head, stalk, and tail.
  • ATP binds to the head and alters the shape to move along microtubules.

Motor Protein Toolbox

  • There are different types of kinesins and myosins.
  • Myosin and kinesin are involved in cellular transport.

Myosin Phylogeny

  • Myosin is a protein family, and there is a phylogenetic tree showing the relationships between different types of myosins.

Muscle Organization

  • Muscles attach to bones by tendons.
  • Tendons attach to bone, and ligaments attach to bone.
  • Muscles are made up of fascicles.
  • Fascicles are a bundle of muscle fibers (cells).
  • Muscle fibers (cells) are surrounded by endomysium.
  • Perimysium surrounds fascicles.
  • Epimysium surrounds all the fascicles.
  • At the cellular level, the individual muscle cells are called muscle fibers or cells.

Muscle Fibers

  • Muscle fibers (cells) have many nuclei.
  • Muscle fibers contain many myofibrils.

Types of Muscle Tissue

  • Skeletal muscle: striated, voluntary movement
  • Smooth muscle: not striated, involuntary control
  • Cardiac muscle: striated, involuntary control

Sarcomere

  • The sarcomere is the functional unit of skeletal muscles.
  • The sarcomere contains thick and thin filaments.
  • The thick filaments are primarily composed of myosin.
  • Thin filaments are primarily composed of actin.
  • The Z line is on the ends of the sarcomere. This stabilizes the filaments.

Sliding Filament Theory

  • The Sliding Filament Theory describes how muscles contract.
  • Myosin heads bind to actin.
  • Myosin heads pivot and pull thin filaments.
  • Sarcomeres shorten.
  • The theory was proposed by Huxley and Niedergerke in 1954.

Muscle Contraction

  • The force of muscle contraction is dependent on the interactions between actin and myosin.
  • ATP drives the sliding contraction of filament.
  • Calcium ions (Ca2+) are essential mediators by triggering the interaction between actin and myosin.

Thick Filament

  • Myosin molecules are arranged hexagonally in thick filaments.
  • There are ~300 myosin molecules in each section of thick filaments.
  • Myosin head groups bind to actin molecules.

Thin Filaments

  • Actin monomers form thin filaments.
  • These filaments contain tropomyosin and troponin complexes.
  • These proteins control the interaction between actin and myosin.

Myosin Head

  • Myosin heads have ATP binding sites which also drive the myosin head group movement.

Cross-Bridge Cycle

  • ATP hydrolysis releases energy which causes myosin head to pivot.
  • ADP and phosphate from ATP bind and are released throughout the cycle.

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