Podcast
Questions and Answers
What is the primary role of motor proteins in relation to the cytoskeleton?
What is the primary role of motor proteins in relation to the cytoskeleton?
- To synthesize proteins
- To support cell shape
- To facilitate cellular movement (correct)
- To generate energy
Kinesin and dynein are both types of motor proteins that transport materials along microtubules.
Kinesin and dynein are both types of motor proteins that transport materials along microtubules.
True (A)
What are the two main types of protein structures that make up the cytoskeleton?
What are the two main types of protein structures that make up the cytoskeleton?
Microtubules and microfilaments
Myosin interacts with ATP and exists mostly as a ______.
Myosin interacts with ATP and exists mostly as a ______.
Match the types of motor proteins to their primary function:
Match the types of motor proteins to their primary function:
What is the primary purpose of T-tubules in muscle fibers?
What is the primary purpose of T-tubules in muscle fibers?
The sliding filament theory was proposed by Hugh Huxley in 1954.
The sliding filament theory was proposed by Hugh Huxley in 1954.
What are the three components of the troponin complex?
What are the three components of the troponin complex?
The _______ is the basic functional unit of a muscle fiber.
The _______ is the basic functional unit of a muscle fiber.
Match the following muscle contraction proteins with their roles:
Match the following muscle contraction proteins with their roles:
What type of muscle tissue is under voluntary control?
What type of muscle tissue is under voluntary control?
Smooth muscle tissue contracts involuntarily.
Smooth muscle tissue contracts involuntarily.
What structure attaches muscles to bones?
What structure attaches muscles to bones?
Muscle fibers are wrapped in __________.
Muscle fibers are wrapped in __________.
Match the muscle types with their characteristics:
Match the muscle types with their characteristics:
What muscle type is responsible for the contractions of the heart?
What muscle type is responsible for the contractions of the heart?
All muscle tissues contain a single nucleus.
All muscle tissues contain a single nucleus.
What is the role of actin and myosin in muscle contraction?
What is the role of actin and myosin in muscle contraction?
What is the primary functional unit of muscle contraction?
What is the primary functional unit of muscle contraction?
The I band corresponds with the region where myosin is found.
The I band corresponds with the region where myosin is found.
Name the protein that stabilizes actin filaments.
Name the protein that stabilizes actin filaments.
The thick filament in a sarcomere is primarily made of _____ molecules.
The thick filament in a sarcomere is primarily made of _____ molecules.
Which protein stops the depolymerization of actin filaments?
Which protein stops the depolymerization of actin filaments?
Match the following components of muscle contraction with their functions:
Match the following components of muscle contraction with their functions:
Describe how the sarcomere is shortened during contraction.
Describe how the sarcomere is shortened during contraction.
In a sarcomere, each thick filament is surrounded by six thin filaments.
In a sarcomere, each thick filament is surrounded by six thin filaments.
What is the role of tropomyosin in muscle contraction?
What is the role of tropomyosin in muscle contraction?
Troponin and tropomyosin are involved in exposing the active sites on actin during muscle contraction.
Troponin and tropomyosin are involved in exposing the active sites on actin during muscle contraction.
What is needed for the myosin head group to detach from the actin filament?
What is needed for the myosin head group to detach from the actin filament?
The _____ ion binds to troponin to initiate muscle contraction.
The _____ ion binds to troponin to initiate muscle contraction.
Match the following terms with their corresponding roles:
Match the following terms with their corresponding roles:
What happens to tropomyosin when calcium ions bind to troponin?
What happens to tropomyosin when calcium ions bind to troponin?
Without ATP, myosin heads will detach from the actin filaments.
Without ATP, myosin heads will detach from the actin filaments.
What occurs during the cross-bridge attachment phase?
What occurs during the cross-bridge attachment phase?
What happens during the pivoting step of muscle contraction?
What happens during the pivoting step of muscle contraction?
Myosin is permanently bound to actin until ATP is released.
Myosin is permanently bound to actin until ATP is released.
What is released when myosin splits ATP?
What is released when myosin splits ATP?
The __________ step involves the myosin head pivoting toward the center of the sarcomere.
The __________ step involves the myosin head pivoting toward the center of the sarcomere.
Match the following steps of muscle contraction with their descriptions:
Match the following steps of muscle contraction with their descriptions:
What is required for myosin reactivation?
What is required for myosin reactivation?
The force of contraction decreases as calcium concentration increases.
The force of contraction decreases as calcium concentration increases.
What role does ATP play in muscle contraction?
What role does ATP play in muscle contraction?
Flashcards
Cytoskeleton
Cytoskeleton
Internal framework of a cell, made of microtubules and microfilaments, providing structural support, enabling movement, and transporting materials.
Motor Proteins
Motor Proteins
Proteins that use energy from ATP to move along cytoskeletal filaments, like microtubules and microfilaments, carrying cargo and contributing to cellular movement.
Kinesin
Kinesin
A type of motor protein that moves cargo towards the plus end of microtubules, usually towards the cell's periphery.
Dynein
Dynein
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Myosin
Myosin
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Skeletal Muscle
Skeletal Muscle
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Smooth Muscle
Smooth Muscle
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Cardiac Muscle
Cardiac Muscle
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Sarcomere
Sarcomere
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Actin
Actin
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Muscle Contraction
Muscle Contraction
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Tendon
Tendon
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What is a sarcomere?
What is a sarcomere?
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What is the myosin filament?
What is the myosin filament?
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What is the A band?
What is the A band?
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What is the I band?
What is the I band?
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What is tropomyosin?
What is tropomyosin?
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What is troponin?
What is troponin?
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What is tropomodulin?
What is tropomodulin?
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How does a sarcomere shorten?
How does a sarcomere shorten?
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Sarcoplasmic Reticulum
Sarcoplasmic Reticulum
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T-tubules
T-tubules
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Sliding Filament Theory
Sliding Filament Theory
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Troponin-Tropomyosin Complex
Troponin-Tropomyosin Complex
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Myosin Head Pivoting
Myosin Head Pivoting
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Myosin Detachment
Myosin Detachment
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Myosin Reactivation
Myosin Reactivation
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Calcium and Muscle Tension
Calcium and Muscle Tension
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Muscle Tension Regulation
Muscle Tension Regulation
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Regulating Muscle Tension
Regulating Muscle Tension
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What does tropomyosin do when a muscle is at rest?
What does tropomyosin do when a muscle is at rest?
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What is the role of troponin in muscle contraction?
What is the role of troponin in muscle contraction?
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Explain the first step of muscle contraction: Active Site Exposure.
Explain the first step of muscle contraction: Active Site Exposure.
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What happens during the second step of muscle contraction: Cross-Bridge Attachment?
What happens during the second step of muscle contraction: Cross-Bridge Attachment?
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What is the role of ATP in muscle contraction?
What is the role of ATP in muscle contraction?
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Explain the third step of muscle contraction: Cross-Bridge Detachment.
Explain the third step of muscle contraction: Cross-Bridge Detachment.
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What happens during the fourth step of muscle contraction: Myosin Head Readies for Another Cycle?
What happens during the fourth step of muscle contraction: Myosin Head Readies for Another Cycle?
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How do the four steps of muscle contraction result in muscle fiber shortening?
How do the four steps of muscle contraction result in muscle fiber shortening?
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Study Notes
Motor Proteins
- Motor proteins are involved in movement within cells.
- Microtubules and microfilaments are structures in cells made up of proteins (actin and tubulin).
- Organisms move in three ways:
- reorganizing cytoskeleton
- carrying things around cells along microtubules with motor proteins
- pulling on elements of cytoskeleton by motor proteins
Motor Protein Kinesin
- Kinesin is a motor protein that moves vesicles along microtubules.
- Kinesin carries cargo to the microtubule-organizing center (MTOC).
- Kinesin operates along microtubules using ATP (adenosine triphosphate) as fuel.
Motor Protein Dynein
- Dynein is another motor protein.
- Dynein carries cargo back to the microtubule organizing center (MTOC).
- Dynein operates along microtubules using ATP.
Structure of Kinesin
- Kinesin has a head, stalk, and tail.
- ATP binds to the head and alters the shape to move along microtubules.
Motor Protein Toolbox
- There are different types of kinesins and myosins.
- Myosin and kinesin are involved in cellular transport.
Myosin Phylogeny
- Myosin is a protein family, and there is a phylogenetic tree showing the relationships between different types of myosins.
Muscle Organization
- Muscles attach to bones by tendons.
- Tendons attach to bone, and ligaments attach to bone.
- Muscles are made up of fascicles.
- Fascicles are a bundle of muscle fibers (cells).
- Muscle fibers (cells) are surrounded by endomysium.
- Perimysium surrounds fascicles.
- Epimysium surrounds all the fascicles.
- At the cellular level, the individual muscle cells are called muscle fibers or cells.
Muscle Fibers
- Muscle fibers (cells) have many nuclei.
- Muscle fibers contain many myofibrils.
Types of Muscle Tissue
- Skeletal muscle: striated, voluntary movement
- Smooth muscle: not striated, involuntary control
- Cardiac muscle: striated, involuntary control
Sarcomere
- The sarcomere is the functional unit of skeletal muscles.
- The sarcomere contains thick and thin filaments.
- The thick filaments are primarily composed of myosin.
- Thin filaments are primarily composed of actin.
- The Z line is on the ends of the sarcomere. This stabilizes the filaments.
Sliding Filament Theory
- The Sliding Filament Theory describes how muscles contract.
- Myosin heads bind to actin.
- Myosin heads pivot and pull thin filaments.
- Sarcomeres shorten.
- The theory was proposed by Huxley and Niedergerke in 1954.
Muscle Contraction
- The force of muscle contraction is dependent on the interactions between actin and myosin.
- ATP drives the sliding contraction of filament.
- Calcium ions (Ca2+) are essential mediators by triggering the interaction between actin and myosin.
Thick Filament
- Myosin molecules are arranged hexagonally in thick filaments.
- There are ~300 myosin molecules in each section of thick filaments.
- Myosin head groups bind to actin molecules.
Thin Filaments
- Actin monomers form thin filaments.
- These filaments contain tropomyosin and troponin complexes.
- These proteins control the interaction between actin and myosin.
Myosin Head
- Myosin heads have ATP binding sites which also drive the myosin head group movement.
Cross-Bridge Cycle
- ATP hydrolysis releases energy which causes myosin head to pivot.
- ADP and phosphate from ATP bind and are released throughout the cycle.
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