Podcast
Questions and Answers
The muscle you can see on the microscope screen was stained for myosin ATPase and a darker stain indicates a higher capacity to use ATP. This means muscles can contract more quickly and can be considered fast twitch. Which of the three cells on the microscope screen can be considered fast twitch?
The muscle you can see on the microscope screen was stained for myosin ATPase and a darker stain indicates a higher capacity to use ATP. This means muscles can contract more quickly and can be considered fast twitch. Which of the three cells on the microscope screen can be considered fast twitch?
- 2 and 3 (correct)
- 1, 2, and 3
- 1
Of the three cells on the microscope screen, which have the potential to be more oxidative?
Of the three cells on the microscope screen, which have the potential to be more oxidative?
- 1, 2, and 3
- 1 and 2 (correct)
- 2 and 3
- 1
Using this information, which of the three cells on the microscope screen has a high capacity for anaerobic activity?
Using this information, which of the three cells on the microscope screen has a high capacity for anaerobic activity?
- 1, 2, and 3
- 1
- 1 and 2
- 2 and 3 (correct)
Now it's time to put these three assays together. Looking at the table, what type of fiber is cell 1?
Cell 1
Cell 2
Cell 3
mATPase
light
dark
dark
SDH
dark
medium
light
aGPDH
light
dark
dark
Now it's time to put these three assays together. Looking at the table, what type of fiber is cell 1?
| Cell 1 | Cell 2 | Cell 3 | |
|---|---|---|---|
| mATPase | light | dark | dark |
| SDH | dark | medium | light |
| aGPDH | light | dark | dark |
Looking at the table, what type of fiber is cell 2?
Cell 1
Cell 2
Cell 3
mATPase
light
dark
dark
SDH
dark
medium
light
aGPDH
light
dark
dark
Looking at the table, what type of fiber is cell 2?
| Cell 1 | Cell 2 | Cell 3 | |
|---|---|---|---|
| mATPase | light | dark | dark |
| SDH | dark | medium | light |
| aGPDH | light | dark | dark |
Finally, looking at the table, what type of fiber is cell 3?
Cell 1
Cell 2
Cell 3
mATPase
light
dark
dark
SDH
dark
medium
light
aGPDH
light
dark
dark
Finally, looking at the table, what type of fiber is cell 3?
| Cell 1 | Cell 2 | Cell 3 | |
|---|---|---|---|
| mATPase | light | dark | dark |
| SDH | dark | medium | light |
| aGPDH | light | dark | dark |
Based solely on the fiber types that you've found within these muscles, which muscle seems to be more suited to slower, sustained movements like walking?
Based solely on the fiber types that you've found within these muscles, which muscle seems to be more suited to slower, sustained movements like walking?
From the graphs you have made that are visible on the centre screen, how does passive tension change with muscle length?
From the graphs you have made that are visible on the centre screen, how does passive tension change with muscle length?
How does active twitch tension change with muscle length?
How does active twitch tension change with muscle length?
Comparing the two graphs, which of the following could possibly explain the faster contraction and relaxation speeds of the EDL relative to the soleus?
Comparing the two graphs, which of the following could possibly explain the faster contraction and relaxation speeds of the EDL relative to the soleus?
Take a look at the graphs. Why might the EDL generate a higher force during a single twitch, but the two muscles generate similar forces when stimulated at 40 Hz?
Take a look at the graphs. Why might the EDL generate a higher force during a single twitch, but the two muscles generate similar forces when stimulated at 40 Hz?
Flashcards
Darker muscle stain
Darker muscle stain
Indicates a higher capacity to use ATP, enabling faster muscle contractions.
Intensely stained cells
Intensely stained cells
Cells heavily stained for SDH have more mitochondria and can be considered more oxidative.
Cells more glycolytic
Cells more glycolytic
These cells are more glycolytic, function well under anaerobic conditions, and produce more lactic acid from glycolysis.
Cell 1 characteristics
Cell 1 characteristics
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Cell 2 characteristics
Cell 2 characteristics
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Cell 3 characteristics
Cell 3 characteristics
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Soleus Muscle
Soleus Muscle
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Passive tension
Passive tension
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How passive tension changes
How passive tension changes
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Active twitch tension
Active twitch tension
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Sarcoplasmic reticulum
Sarcoplasmic reticulum
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EDL's Calcium Release
EDL's Calcium Release
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Study Notes
- The muscle on the microscope screen is stained for myosin ATPase, with a darker stain indicating higher ATP usage capacity, and faster twitch muscles.
- The correct answer is cells 2 and 3 because they are darker, which means they contain more of the enzyme mATPase.
- Cells 1 and 2 have higher oxidative potential, which is the correct answer.
- Cells 2 and 3 have a high capacity for anaerobic activity, which is the correct answer. Because glycolytic cells work well under anaerobic conditions and produce more lactic acid from glycolysis.
- The correct fiber type for cell 1 is slow and oxidative. Cell 1 stained lightly using mATPase (slow twitch) and dark for SDH in mitochondria (oxidative). It did not stain much for aGDPH (not very glycolytic).
- Cell 2 is fast, oxidative, and glycolytic, which is the correct answer. Cell 2 stained strongly for all three protocols indicating it is fast twitch, contains some mitochondria, and is functional under anaerobic conditions.
- Cell 3 is fast and glycolytic. Cell 3 stained for both mATPase and aGDPH (meaning it can convert ATP, and work under anaerobic conditions well) and didn't stain much for SDH (fewer mitochondria and is non-oxidative).
- The soleus muscle is more suited to slower, sustained movements.
- The correct answer is the soleus; it stained strongly using SDH staining, meaning it's filled with mitochondria, which are used for aerobic work. The EDL is for anaerobic and fast movements, like sprinting.
- Passive tension increases with muscle length
- Active twitch tension increases, then decreases with muscle length.
- The faster contraction and relaxation speeds of the EDL compared to the soleus are due to the EDL having more sarcoplasmic reticulum.
- The EDL can release more calcium with a single twitch, but at 40 Hz, both muscles achieve similar cytoplasmic calcium levels. The soleus releases calcium to the cytoplasm and pumps it back to the sarcoplasmic reticulum more slowly than the EDL during a twitch, but at 40 Hz, enough calcium is released from the sarcoplasmic reticulum to achieve much higher forces.
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