Müller-Hinton Agar Procedure Quiz

The purpose of sterilizing MHA is to eliminate any contaminants and ensure a pure medium for microbial testing. It is sterilized by autoclaving at 15 lbs pressure and 121°C for 15 minutes.

To prepare MHA, 38g of the medium is dissolved in 1000ml of distilled water by heating until boiling, then sterilized by autoclaving before cooling to 45-50°C for pouring into sterile plates.

Müller-Hinton Agar can be used to isolate and maintain Neisseria and Moraxella species.

<p>The control organism used is E. coli with the ATCC number 25922.</p> Signup and view all the answers

<p>The inhibition zone indicates the effectiveness of an antimicrobial agent against a microorganism; a larger zone reflects greater sensitivity.</p> Signup and view all the answers

<p>38g</p> Signup and view all the answers

<p>15</p> Signup and view all the answers

<p>rapidly</p> Signup and view all the answers

<p>25922</p> Signup and view all the answers

<p>Moraxella</p> Signup and view all the answers

Antimicrobial susceptibility testing for nonfastidious, rapidly-growing microbes; isolating and maintaining Neisseria/Moraxella species.

Dissolve 38g of MHA in 1000ml distilled water by heating to boiling.

Autoclave at 15 lbs pressure, 121°C for 15 minutes.

Escherichia coli (ATCC 25922)

Cool to 45-50°C before pouring into plates.

MHA (Müller-Hinton Agar) is used to test how well antibiotics work against bacteria (antimicrobial susceptibility testing), especially for fast-growing bacteria that aren't picky about their environment. It's also used to grow and keep Neisseria and Moraxella bacteria.

MHA is sterilized by autoclaving, which means heating it under high pressure (15 pounds) at 121°C for 15 minutes. This kills any unwanted microbes.

After sterilizing, MHA needs to cool down to between 45-50°C before pouring into Petri dishes. This ensures the agar doesn't burn the bacteria.

Control organisms like E. coli, S. aureus, and P. aeruginosa are used to make sure the MHA is working properly. They are known to react in specific ways to antibiotics, so they help determine if the test is accurate.

The growth of bacteria and the size of the inhibition zone around antibiotic discs are compared to standard charts. This helps determine if the bacteria is susceptible, resistant, or intermediate to the antibiotic.