9-L1

What is the ultimate analytical assay for DNA?

DNA polymerase
Gel electrophoresis
DNA sequencing (correct)
PCR products

When was DNA sequencing fully established?

1980s
1970s
1975 (correct)
1960s

What is the study of entire genomes of organisms?

Genetics
Genomics (correct)
Molecular Biology
Proteomics

Why is DNA sequencing important?

To detect variations at DNA segment (C) Signup and view all the answers

What is required for automated DNA sequencing?

All of the above (D) Signup and view all the answers

What is the function of dideoxynucleotides in DNA sequencing?

To terminate PCR fragments (B) Signup and view all the answers

What is the purpose of gel electrophoresis in DNA sequencing?

To separate PCR fragments by length (C) Signup and view all the answers

What is the outcome of each PCR fragment in DNA sequencing?

A colour-labeled ddNTP (D) Signup and view all the answers

What is the process of identifying a disease by studying molecules?

Molecular Diagnosis (A) Signup and view all the answers

At which level does DNA sequencing occur in DNA analysis?

Molecular level (C) Signup and view all the answers

What is the purpose of Restriction Endonucleases?

To recognize and cut DNA at specific sequences (A) Signup and view all the answers

What is the result of Restriction enzyme action on DNA?

Creation of either sticky or blunt ends (B) Signup and view all the answers

What is the characteristic of Restriction sites?

They are palindromic sequences (D) Signup and view all the answers

What is the purpose of Gel electrophoresis in DNA analysis?

To separate DNA fragments based on size (A) Signup and view all the answers

What is the technique used to analyze DNA at the chromosomal level?

Karyotyping (A) Signup and view all the answers

What is the result of Polymerase Chain Reaction (PCR)?

Amplification of DNA sequences (A) Signup and view all the answers

What is the recognition sequence of the Eco RI enzyme?

GAATTC (D) Signup and view all the answers

What is the main purpose of gene cloning?

To create recombinant DNA molecules (A) Signup and view all the answers

Which of the following is an application of recombinant DNA technology?

Production of therapeutic proteins (D) Signup and view all the answers

What is the purpose of recombinant DNA technology in disease prevention?

To produce vaccines (A) Signup and view all the answers

Which of the following is a product of recombinant DNA technology?

Therapeutic proteins (C) Signup and view all the answers

What is the recognition sequence of the Bam HI enzyme?

GGATCC (A) Signup and view all the answers

What is the purpose of the size markers in Lane 1 of the Southern blotting process?

To provide a standard for measuring the size of DNA fragments (B) Signup and view all the answers

What is the purpose of linking DNA fragments to self-replicating vectors?

To create recombinant DNA molecules (B) Signup and view all the answers

What is the function of the buffer in the Southern blotting process?

To transfer the DNA fragments to the filter (C) Signup and view all the answers

What is the primary basis for separating macromolecules in gel electrophoresis?

Size or charge (A) Signup and view all the answers

What is the purpose of the filter in the Southern blotting process?

To bind the DNA fragments (D) Signup and view all the answers

What happens to DNA molecules when placed in an electric field?

They move towards the positively charged electrode (A) Signup and view all the answers

Why do smaller DNA fragments migrate faster than larger ones during gel electrophoresis?

Because they are smaller in size (B) Signup and view all the answers

What is the purpose of the X-ray film in the Southern blotting process?

To visualize the DNA fragments (D) Signup and view all the answers

What is the purpose of PCR?

To amplify specific segments of DNA (C) Signup and view all the answers

What is the purpose of staining DNA with a fluorescent dye after gel electrophoresis?

To visualize the DNA under UV light (C) Signup and view all the answers

What is the direction of movement of DNA fragments during gel electrophoresis?

From the negative electrode to the positive electrode (C) Signup and view all the answers

What is the advantage of PCR over other molecular biology techniques?

It can amplify specific segments of DNA from a very small sample (A) Signup and view all the answers

What is the function of the power supply in gel electrophoresis?

To provide an electric charge (A) Signup and view all the answers

In which direction do the smallest DNA fragments migrate during gel electrophoresis?

Towards the positive electrode (C) Signup and view all the answers

What is the purpose of the heat-sealed food bag in the Southern blotting process?

To contain the solution with the labeled probe (C) Signup and view all the answers