Molecular Cell Biology II: Plastid Evolution

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Questions and Answers

Which pigment is NOT associated with Heterokontophyta?

  • Peridinin (correct)
  • Chlorophyll a
  • Fucoxanthin
  • Chlorophyll c

What is the primary storage compound found in Heterokontophyta?

  • Starch
  • Cellulose
  • Glycogen
  • Chrysolaminarin (correct)

Which class of algae is known for its silica cell wall?

  • Xanthophyceae
  • Chrysophyceae
  • Phaeophyta
  • Bacillariophyceae (correct)

Which of the following describes the flagella of Haptophyta?

<p>Two flagella (A)</p> Signup and view all the answers

Which pigment is primarily involved in photosynthesis for Dinophyta?

<p>Chlorophyll a (D)</p> Signup and view all the answers

What type of algae is commonly referred to as 'Golden algae'?

<p>Chrysophyceae (D)</p> Signup and view all the answers

What type of feeding strategy do dinoflagellates exhibit when they combine both photosynthesis and ingestion of organic material?

<p>Mixotrophic (D)</p> Signup and view all the answers

What is a distinct feature of diatoms in terms of their cellular structure?

<p>Silica frustules (B)</p> Signup and view all the answers

What structural feature provides dinoflagellates with protection and classification characteristics?

<p>Theca (B)</p> Signup and view all the answers

Which group of algae is indicated as important for primary production in the oceans?

<p>Bacillariophyceae (C)</p> Signup and view all the answers

Which flagellum of dinoflagellates lies in a groove and facilitates a spinning motion?

<p>Transverse Flagellum (D)</p> Signup and view all the answers

Which of the following is NOT a characteristic of dinoflagellates?

<p>Being exclusively autotrophic (B)</p> Signup and view all the answers

What significant role do dinoflagellates serve in aquatic ecosystems?

<p>Primary producers (B)</p> Signup and view all the answers

Which characteristic distinguishes the dinoflagellate's unique swimming pattern?

<p>Spiraling pattern (C)</p> Signup and view all the answers

Which of the following dinoflagellates is known for causing red tide?

<p>Karenia brevis (D)</p> Signup and view all the answers

What type of relationship do zooxanthellae have with corals?

<p>Mutualistic (C)</p> Signup and view all the answers

What is the primary event that led to the formation of mitochondria in eukaryotic cells?

<p>Phagocytosis of an ancestral bacteria (D)</p> Signup and view all the answers

Which organelles evolved from the uptake of cyanobacteria by the ancestral eukaryotic cell?

<p>Plastids (B)</p> Signup and view all the answers

What significant process occurred concurrently with the development of mitochondria?

<p>Formation of the nuclear envelope (A)</p> Signup and view all the answers

Which group of organisms did the ancestral eukaryote evolve into?

<p>Both animals and fungi (C)</p> Signup and view all the answers

Which of the following components is NOT a result of the endosymbiotic theory?

<p>Nuclear envelope from nucleus (C)</p> Signup and view all the answers

What evidence supports the theory of serial endosymbiosis?

<p>Presence of DNA in mitochondria and plastids (D)</p> Signup and view all the answers

Which of the following statements about plastid evolution is accurate?

<p>Cyanobacteria were phagocytized by ancestral eukaryotes to form plastids (B)</p> Signup and view all the answers

Which characteristic is shared by both mitochondria and plastids according to the endosymbiotic theory?

<p>They can replicate independently of the host cell (A)</p> Signup and view all the answers

Which structural feature is characteristic of the pigments found in Rhodophyta?

<p>Chlorophyll a and d (B)</p> Signup and view all the answers

What is the storage compound used by Rhodophyta?

<p>Floridean starch (B)</p> Signup and view all the answers

Which of the following is NOT a feature of Cyanophora paradoxa?

<p>Possesses flagellated cells (C)</p> Signup and view all the answers

What type of chloroplast structure is found in Chlorophyta?

<p>Plastid with two membranes (C)</p> Signup and view all the answers

Which enzyme is primarily found in pyrenoids of Chlorophyta species?

<p>Ribulose-1,5-bisphosphate carboxylase/oxygenase (D)</p> Signup and view all the answers

What describes the cell wall composition of Rhodophyta?

<p>Cellulose, mucilage, or CaCO3 (A)</p> Signup and view all the answers

Which of the following is a misconception about the reproduction of Cyanophora paradoxa?

<p>It has both sexual and asexual reproduction methods (A)</p> Signup and view all the answers

What connection is a feature in the cellular structure of Rhodophyta?

<p>Pit connections (C)</p> Signup and view all the answers

What type of endosymbiosis occurs when a primary plastid-bearing alga is ingested by a non-photosynthetic eukaryote?

<p>Secondary endosymbiosis (B)</p> Signup and view all the answers

What structure in secondary plastid-bearing algae provides evidence of the endosymbiont's origin?

<p>Nucleomorph (D)</p> Signup and view all the answers

In what type of algae does the endosymbiont nucleus persist as a nucleomorph?

<p>Chlorarachniophyte and cryptophyte algae (C)</p> Signup and view all the answers

How many membranes characterize secondary plastids?

<p>Three or four membranes (C)</p> Signup and view all the answers

What happens to the genes of both prokaryotic and eukaryotic ancestry during secondary endosymbiosis?

<p>They are transferred to the secondary host nucleus (C)</p> Signup and view all the answers

Which of the following structures is not associated with secondary plastid-bearing algae?

<p>Chlorophyll a (C)</p> Signup and view all the answers

What type of organism typically serves as the secondary host in secondary endosymbiosis?

<p>Non-photosynthetic eukaryote (A)</p> Signup and view all the answers

What is one consequence for certain secondary plastid-bearing algae regarding nucleomorphs?

<p>Nucleomorphs have been lost in some algae (C)</p> Signup and view all the answers

What is the significance of supernumerary membranes in plastids?

<p>They are evidence of secondary endosymbiosis. (B)</p> Signup and view all the answers

Which of the following groups is described as having plastids that evolved from cyanobacteria?

<p>Archaeplastida (A), Green algae (B), Euglena (C), Glaucophyta (D)</p> Signup and view all the answers

What is the role of pigments like chlorophyll a and phycobiliproteins in Glaucophyta?

<p>They function in photosynthesis. (C)</p> Signup and view all the answers

Which of the following best describes the relationship between endosymbiosis and the evolution of plastids?

<p>Plastids are a result of endosymbiotic events. (D)</p> Signup and view all the answers

What was the primary hypothesis regarding plastid evolution prior to the findings in the 1970s and 80s?

<p>Plastids were thought to have multiple independent origins. (B)</p> Signup and view all the answers

In what way did proponents of endosymbiosis use plastid diversity to support their claims?

<p>Diversity indicated multiple instances of endosymbiosis. (D)</p> Signup and view all the answers

What morphological diversity is observed in plastids among algae?

<p>Plastids in algae can have varying numbers of membranes. (B)</p> Signup and view all the answers

What significant ecological impact has resulted from secondary endosymbiosis in algae?

<p>It has contributed to the emergence of new algal lineages. (A)</p> Signup and view all the answers

Flashcards

Endosymbiosis

The process by which a eukaryotic cell engulfs and incorporates another organism, such as a bacterium, within its cytoplasm.

Serial Endosymbiosis

The evolutionary origin of mitochondria and chloroplasts through endosymbiotic events.

Ur-Eucyte

An ancestral eukaryotic cell that gave rise to animals and fungi.

Mitochondrial Endosymbiosis

The theory that suggests the mitochondria in eukaryotic cells originated from the engulfment and symbiosis of a proteobacterium by an ancestral eukaryotic cell.

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Chloroplast Endosymbiosis

The theory that suggests chloroplasts in plants originated from the engulfment of a photosynthetic cyanobacterium by an ancestral eukaryotic cell.

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Cyanobacteria

A type of bacterium that performs photosynthesis.

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Prokaryotes

A group of organisms that includes bacteria and archaea, distinguished by their lack of a nucleus and other membrane-bound organelles.

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Eukaryotes

A group of organisms that includes plants, animals, fungi, and protists, characterized by the presence of a nucleus and other membrane-bound organelles.

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Primary Endosymbiosis

A process where a eukaryotic cell engulfs a prokaryotic cell, which then becomes an organelle within the host cell. This is how eukaryotic cells acquired chloroplasts.

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Archaeplastida

The group of organisms that includes red algae, green algae, and land plants, all of which are descended from a primary endosymbiotic event involving a cyanobacterium.

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Glaucophyta

A type of algae characterized by their small size and the presence of a unique structure called a cyanelle, which is essentially a chloroplast that still retains a peptidoglycan wall like its cyanobacterial ancestor.

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Cyanobacterial OM

The outer membrane of a plastid that originated from the outer membrane of the original cyanobacterium.

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Cyanobacterial IM

The inner membrane of a plastid that originated from the inner membrane of the original cyanobacterium.

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Secondary Endosymbiosis

A secondary endosymbiotic event where a eukaryotic cell engulfs another eukaryotic cell that contains a plastid.

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Nucleomorph

A small, degenerate nucleus that is found in some algae and is thought to be the remnant of the nucleus of the eukaryotic cell that was engulfed during secondary endosymbiosis.

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Cyanophora paradoxa

A type of algae with blue-green plastids called cyanelles, starch stored in the cytoplasm, and does not engage in sexual reproduction.

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Rhodophyta (Red Algae)

A type of algae with chlorophyll a and d, phycocyanin, and phycoerythrin pigments, no flagellated cells, cell walls made up of cellulose, mucilage, or calcium carbonate, and stores floridean starch.

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Chlorophyta (Green Algae)

A type of algae with chlorophylls a and b, stores starch in chloroplasts, has plastids with two membranes, lacks grana stacks in its thylakoid membranes, and has a cell wall mainly composed of cellulose.

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Pyrenoid

A spherical or ellipsoidal body found within the chloroplast of certain algae, mainly composed of RuBisCO, an enzyme crucial for carbon fixation during photosynthesis.

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Paulinella chromatophora

A type of eukaryotic organism known for its ability to incorporate a cyanobacterium into its own structure, resulting in the formation of a chromatophore.

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Chromatophore

The specialized plastid found within Paulinella chromatophora, a photosynthetic organelle derived from a symbiotic cyanobacterium.

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Endosymbiosis of Plastids

Endosymbiosis is a process where one organism (symbiont) lives inside another organism (host) and benefits both. In the case of plastids, a prokaryotic cell, like a cyanobacterium, was engulfed by a eukaryotic host cell. Over time, the symbiont became incorporated into the host cell, eventually becoming a plastid.

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Plastid

A plastid is a type of organelle found in plant cells and some algae. It's responsible for photosynthesis, the process of converting light energy into chemical energy.

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Periplastidial compartment

The periplastidial compartment is a space in the cytoplasm of cells with secondary plastids. It is located between the inner and outer membranes of the plastid and contains the nucleomorphs.

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Inner membrane (IM)

The inner membrane (IM) of a plastid is one of the layers surrounding the plastid. It's derived from the original prokaryotic symbiont's cell membrane.

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Mixotrophic Dinoflagellates

A type of dinoflagellate that combines autotrophic (photosynthesis) and heterotrophic (eating other organisms) behaviors, enabling them to survive under various environmental conditions.

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Cingulum

A groove or indentation around a dinoflagellate cell where the transverse flagellum resides, aiding in spinning motion.

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Longitudinal Flagellum

A long, whip-like structure that extends posteriorly in dinoflagellates, responsible for directional movement.

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Theca

Tough, protective plates made of cellulose that cover the exterior of dinoflagellates, providing structural support and aiding in classification.

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Zooxanthellae

A symbiotic relationship where dinoflagellates (zooxanthellae) live inside corals, providing them with nutrients through photosynthesis; in return, the corals offer shelter and CO2 for photosynthesis.

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Harmful Algal Bloom (HAB) Dinoflagellates

Dinoflagellates that produce toxins and can cause Harmful Algal Blooms (HABs) with significant ecological and economic effects.

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Karenia brevis

A specific type of dinoflagellate responsible for harmful algal blooms known as "red tides."

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Alexandrium species

A group of dinoflagellates that can cause paralytic shellfish poisoning (PSP) by producing toxins.

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Heterokontophyta

A group of algae characterized by heterokont flagella (stramenopiles), chlorophyll a, c, and fucoxanthin pigments, and chrysolaminarin as their storage compound.

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Fucoxanthin

A type of accessory pigment found in heterokontophytes, giving them a brown color. It absorbs light energy and plays a role in photosynthesis.

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Heterokont flagella

A unique type of flagella with two different parts found in heterokontophytes. They are used for movement and may have different structures.

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Chrysolaminarin

A polysaccharide (carbohydrate) used as a storage compound by heterokontophytes. It is stored in cytoplasmic vesicles.

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Phaeophyta

A group of algae within the Heterokontophyta known as 'brown algae'. They are often multicellular and play a critical role in marine ecosystems.

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Chrysophyceae

A class of algae within the Heterokontophyta known as 'golden algae'. These are usually single-celled and often found in freshwater environments.

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Xanthophyceae

A class of algae within the Heterokontophyta known as 'yellow-green algae'. They are often found in freshwater environments and can be either single-celled or filamentous.

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Bacillariophyceae

A class of algae within the Heterokontophyta known as 'diatoms'. They are one of the most abundant algal groups in the world, especially in marine environments.

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Study Notes

Plastid Evolution and Algal Diversity

  • Lecture details: Molecular Cell Biology II (Molecular Cell Biology of Plants), MMLS.G3 (MCB W 15), November 8th + 15th 2024 (WS 2024/25), Jun.-Prof. Dr. Julie Zedler, Synthetic Biology of photosynthetic Organisms, Matthias Schleiden Institute, Faculty of Biosciences

Algal Phylogeny

  • A circular diagram displays the evolutionary relationships among diverse algal lineages.
    • Various groups, including Alveolates, Stramenopiles, Rhizaria, Excavates, Opisthokonts, Archaeplastida (green, red, glaucophytes), and others, are connected in a branching tree-like structure.
    • Each group is associated with specific algal examples and characteristics.
    • The diagram illustrates evolutionary links and diversifications within different algal lineages.
    • This diagram visually represents algal diversity and evolutionary relationships

Serial Endosymbiosis

  • A diagram depicts the process of serial endosymbiosis, showing how eukaryotic cells originated by engulfing and incorporating other cells.
  • The process begins with an ancestral prokaryotic cell that engulfs a cyanobacterium (a photosynthetic bacterium).
  • This interaction leads to the evolution of the first photosynthetic eukaryote.

Endosymbiosis and Plastid Evolution

  • Diagram A illustrates the primary endosymbiosis event where a eukaryotic cell engulfs a cyanobacterium, resulting in a plastid-containing cell.
  • Diagram B illustrates the secondary endosymbiosis event where a eukaryotic cell engulfs a photosynthetic eukaryote, resulting in a plastid with several membranes.

Primary Endosymbiosis Lineages

  • Diagram of the primary endosymbiosis events that led to the evolution of plastids in plants.
  • Shows the lineage(s) of the eukaryotic ancestor and the cyanobacterium that led to each lineage
    • Glaucophyta, Chlorophyta, and Rhodophyta are shown as evolving from a primary endosymbiosis event.

Glaucophyta

  • Chlorophyll a, phycobilins
  • Cyanelles (muroplasts)
  • Starch in cytoplasm
  • No known sexual reproduction

Rhodophyta

  • Chlorophyll a (+d), phycocyanin, phycoerythrin
  • No flagellated cells
  • Cell walls: Cellulose, mucilage (agars, carrageenans) or CaCO3
  • Floridean starch as storage compound
  • Pit connections

Chlorophyta

  • Chlorophyll a, b
  • Starch in chloroplast (+ pyrenoid, also a structure within the chloroplast that is involved in the process of carbon fixation)
  • Plastid with two membranes
  • Thylakoid membranes: no grana stacks
  • Cell wall: mainly cellulose

Life Cycle Stages of Chlamydomonas reinhardtii

  • A diagram illustrating the stages of asexual and sexual reproduction in the alga, including vegetative cells, germ cells, mitosis, meiosis, zygotes and different cell types.

Algae Lineages

  • Diagram illustrating primary and secondary endosymbiotic events that led to the diversity of plastids in algae.
  • Shows the origin of various algal lineages (Chlorophyta, Glaucophyta, Rhodophyta, Cryptophyta, Euglenophyta, Dinophyta, Heterokontophyta, etc.).

Chlorarachniophyta

  • Chlorophyll a, b
  • Nucleomorph between chloroplast and ER membrane (in total four membranes)
  • Storage compound: paramylon, sometimes amoeboflagellates

Euglenophyta

  • Mainly unicellular flagellates (2 flagella)
  • Mostly freshwater organisms
  • Mixotrophic species, many heterotrophic species
  • Plastids with 3 membranes
  • Pigments: Chlorophyll a, b
  • Storage compound: paramylon
  • No cell wall
  • Pellicle: a flexible covering on the surface of the cell that allows for euglenoid movement, which is a characteristic type of movement

Cryptophyta

  • Pigments: chlorophyll a, c, phycobiliproteins
  • Nucleomorph
  • Periplast inside plasma membrane
  • Starch product between chloroplast membrane
  • Ejectosome

Heterokontophyta

  • Pigments: chlorophyll a, c, fucoxanthin
  • Heterokont flagella (stramenopiles)
  • Storage compound: chrysolaminarin (cytoplasmic vesicles)
  • 4 chloroplast membrane (chloroplast ER), nucleomorph lost
  • Phaeophyta (“brown algae") (also includes diatoms, golden algae, yellow-green algae)

Chrysophyta

  • Chrysophyceae: "Golden algae"
  • Xanthophyceae: “Yellow-green algae"

Bacillariophyceae ("Diatoms")

  • Most important algal class for primary production in the oceans
  • Silica cell wall (frustule)
  • Unicellular, some colonial
  • Pennales vs. Centrales

Life Cycle Centric Diatoms

  • Diagram showing the stages of the diatom life cycle, including oogenesis, spermatogenesis, vegetative growth, sexual reproduction, and auxospore development.

Haptophyta (Prymnesiophyta)

  • Two flagella
  • Pigments: chlorophyll a, c, fucoxanthin
  • Coccoliths: Scales outside cell (CaCO3, cellulose)
  • Storage product: chrysolaminarin (cytoplasmic vesicles)
  • Haptonema

Dinophyta

  • Pigments: chlorophyll a, b + carotene/ c + peridinin /c + fucoxanthin
  • Two flagella
  • Theca (cellulose plates)
  • Heterotrophic, autotrophic, mixotrophic
  • Second most important algal class of phytoplankton (after diatoms)
  • Zooxanthellae
  • Highly toxic species (HABs)
  • Bioluminescence

Project "Lohafex"

  • Fertilization of ocean with iron
  • Project in 2009 (Alfred Wegener Institute, National Institute of Oceanography India)
  • 20 t FeSO4 spread over 300 km2

Further Reading

  • List of relevant scientific publications on endosymbiosis, eukaryotic cell evolution, algal phylogeny, and related topics.

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