Podcast
Questions and Answers
What enzyme is responsible for the elongation of the DNA strand during replication?
What enzyme is responsible for the elongation of the DNA strand during replication?
- Ligase
- Helicase
- Topoisomerase
- Polymerase (correct)
Which process refers to the conversion of DNA into RNA?
Which process refers to the conversion of DNA into RNA?
- Replication
- Translocation
- Transcription (correct)
- Translation
What are the DNA fragments formed on the lagging strand called?
What are the DNA fragments formed on the lagging strand called?
- Leading fragments
- Primase fragments
- Lagging fragments
- Okazaki fragments (correct)
What direction does DNA replication proceed from the origin of replication?
What direction does DNA replication proceed from the origin of replication?
What is the function of sigma factor in transcription?
What is the function of sigma factor in transcription?
Which of the following correctly describes a function associated with the Sec transport system?
Which of the following correctly describes a function associated with the Sec transport system?
What is the role of the lambda repressor in the lifecycle of the lambda phage?
What is the role of the lambda repressor in the lifecycle of the lambda phage?
How does a temperate phage differ from a virulent phage?
How does a temperate phage differ from a virulent phage?
Which method is specifically used for quantifying RNA viruses?
Which method is specifically used for quantifying RNA viruses?
What happens to the lambda phage during the induction process?
What happens to the lambda phage during the induction process?
What is the electron donor in the oxidation reaction involving glucose?
What is the electron donor in the oxidation reaction involving glucose?
Which of the following represents the overall reaction of glucose oxidation?
Which of the following represents the overall reaction of glucose oxidation?
What is the electron acceptor in the given reactions involving iron?
What is the electron acceptor in the given reactions involving iron?
Which statement about reduction potential is correct?
Which statement about reduction potential is correct?
In the context of redox reactions, what does a greater difference in redox potential imply?
In the context of redox reactions, what does a greater difference in redox potential imply?
What defines substrate-level phosphorylation (SLP)?
What defines substrate-level phosphorylation (SLP)?
What is the primary challenge of fermentation?
What is the primary challenge of fermentation?
How are the end products of fermentation characterized?
How are the end products of fermentation characterized?
In the reaction 2H+ + H2 → H2O, what is the value of ΔE if E' accepting couple is 0.82 V and E' donating couple is -0.42 V?
In the reaction 2H+ + H2 → H2O, what is the value of ΔE if E' accepting couple is 0.82 V and E' donating couple is -0.42 V?
What does the formula ΔG' = -nFΔE' represent?
What does the formula ΔG' = -nFΔE' represent?
What is the physiological state of a cell that supports lysogeny?
What is the physiological state of a cell that supports lysogeny?
Which of the following accurately describes prions?
Which of the following accurately describes prions?
How do viroids infect plants?
How do viroids infect plants?
What is the main characteristic of antigenic drift in viruses?
What is the main characteristic of antigenic drift in viruses?
Which type of influenza virus is known for causing pandemics?
Which type of influenza virus is known for causing pandemics?
What defines the difference between antigenic shift and antigenic drift?
What defines the difference between antigenic shift and antigenic drift?
Which surface protein variances are used to classify Type A influenza?
Which surface protein variances are used to classify Type A influenza?
What type of vaccine is classified as an inactivated influenza vaccine?
What type of vaccine is classified as an inactivated influenza vaccine?
What is the overall reaction when glucose is converted to lactic acid?
What is the overall reaction when glucose is converted to lactic acid?
Which enzyme system is essential for ATP synthesis and uses ion flow for catalysis?
Which enzyme system is essential for ATP synthesis and uses ion flow for catalysis?
What is the role of superoxide dismutase (SOD) in organisms?
What is the role of superoxide dismutase (SOD) in organisms?
What distinguishes cyclic photosynthesis from noncyclic photosynthesis?
What distinguishes cyclic photosynthesis from noncyclic photosynthesis?
Which of the following describes a feature of the phosphotransferase system?
Which of the following describes a feature of the phosphotransferase system?
What is the key difference between aerobic and anaerobic respiration in terms of electron acceptors?
What is the key difference between aerobic and anaerobic respiration in terms of electron acceptors?
How does the process of nitrogen fixation primarily occur in microorganisms?
How does the process of nitrogen fixation primarily occur in microorganisms?
Which type of reaction consumes protons inside the cell to create a gradient?
Which type of reaction consumes protons inside the cell to create a gradient?
What is the purpose of using a Na+ gradient in microbes?
What is the purpose of using a Na+ gradient in microbes?
What is required for ATP generation by ATPase?
What is required for ATP generation by ATPase?
Which ion is used by microbes as a temporary alternative to generate gradients in high pH environments?
Which ion is used by microbes as a temporary alternative to generate gradients in high pH environments?
Which of the following elements requires a transport mechanism in Gram-negative bacteria due to its insolubility?
Which of the following elements requires a transport mechanism in Gram-negative bacteria due to its insolubility?
What role does catalase play in microorganisms?
What role does catalase play in microorganisms?
Which of the following statements about NADPH and NADH is correct?
Which of the following statements about NADPH and NADH is correct?
What role do chaperones play in protein processing?
What role do chaperones play in protein processing?
In the Sec system, what is the function of SecB?
In the Sec system, what is the function of SecB?
Which type of protein secretion involves a conjugative transfer system?
Which type of protein secretion involves a conjugative transfer system?
How do enveloped viruses typically release from a host cell?
How do enveloped viruses typically release from a host cell?
During viral replication, what is the role of ligands?
During viral replication, what is the role of ligands?
What occurs immediately after a virus penetrates a host cell?
What occurs immediately after a virus penetrates a host cell?
What is the significance of the retrovirus lifecycle involving reverse transcription?
What is the significance of the retrovirus lifecycle involving reverse transcription?
Which structure is NOT associated with RNA viruses?
Which structure is NOT associated with RNA viruses?
What type of modification can occur during protein processing?
What type of modification can occur during protein processing?
In the viral replication cycle, what is the first step?
In the viral replication cycle, what is the first step?
What is the main function of the SecA protein in the Sec pathway?
What is the main function of the SecA protein in the Sec pathway?
What characterizes Type III protein secretion systems in bacteria?
What characterizes Type III protein secretion systems in bacteria?
Which type of RNA virus replicates its genome in a manner where the genomic RNA serves as mRNA?
Which type of RNA virus replicates its genome in a manner where the genomic RNA serves as mRNA?
Flashcards
Electron Donor
Electron Donor
Substance that loses electrons in a reaction and becomes oxidized.
Electron Acceptor
Electron Acceptor
Substance that gains electrons in a reaction and becomes reduced.
Reduction Potential
Reduction Potential
A measure of a chemical species' tendency to gain electrons and become reduced, expressed in volts.
Proton Motive Force
Proton Motive Force
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ΔE'
ΔE'
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Substrate-Level Phosphorylation (SLP)
Substrate-Level Phosphorylation (SLP)
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Fermentation
Fermentation
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Electron Transport
Electron Transport
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Chemiosmosis
Chemiosmosis
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Electrochemical Gradient
Electrochemical Gradient
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Origin of replication
Origin of replication
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Bidirectional replication
Bidirectional replication
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Leading strand
Leading strand
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Okazaki fragments
Okazaki fragments
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Transcription
Transcription
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Electron Transport Chain
Electron Transport Chain
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ATP Synthase
ATP Synthase
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ATP
ATP
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Lactic Acid Fermentation
Lactic Acid Fermentation
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Redox Reaction
Redox Reaction
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Electronegativity
Electronegativity
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Redox Potential
Redox Potential
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Alcoholic Fermentation
Alcoholic Fermentation
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Photosynthesis
Photosynthesis
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Catabolism
Catabolism
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Anabolism
Anabolism
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Allosteric Regulator
Allosteric Regulator
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Passive Transport
Passive Transport
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Virulent Viral Lifestyle
Virulent Viral Lifestyle
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Temperate/Latent Viral Lifestyle
Temperate/Latent Viral Lifestyle
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Induction (in viral lifecycle)
Induction (in viral lifecycle)
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Temperate Phage
Temperate Phage
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Integration Site of Lambda Phage
Integration Site of Lambda Phage
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Protein Processing
Protein Processing
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Cleavage
Cleavage
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S-S Bond Formation
S-S Bond Formation
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Additions
Additions
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Chaperones
Chaperones
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Trigger Factor
Trigger Factor
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DnaK
DnaK
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GroE
GroE
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Sec System
Sec System
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SecA
SecA
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SecB
SecB
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SecYEG
SecYEG
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Type I: ABC transporter
Type I: ABC transporter
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Type II: Two-step Secretion
Type II: Two-step Secretion
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Viroid
Viroid
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Influenza Type A
Influenza Type A
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Influenza Type B
Influenza Type B
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Influenza Type D
Influenza Type D
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Antigenic Drift
Antigenic Drift
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Antigenic Shift
Antigenic Shift
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Flu Vaccine
Flu Vaccine
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Study Notes
Chapter 5 Metabolism
- Metabolism is the sum of all chemical processes in living systems
- Catabolism is involved in energy generation
- Anabolism is involved in biosynthesis
- Cells require elements like carbon, nitrogen, sulfur, and water, as well as energy from redox reactions and reducing equivalents (H+ + e-)
- Energy carriers like NAD+/NADH and NADP+/NADPH are vital for metabolic processes.
- Organisms are classified based on their energy (chemo vs. photo) and carbon (hetero vs. auto) sources
Life is Redox
- Delta G is the free energy of a reaction.
- Delta G < 0, the reaction is exergonic (releases energy)
- Delta G > 0, the reaction is endergonic (requires energy)
- Standard conditions for redox reactions are: concentration = 1 M, atmospheric pressure = 1 atm, and temperature = 25°C (standard temperature and pressure) ; standard pH =7
- Oxidation is the removal of electrons from a substrate
- Reduction is the addition of electrons to a substrate
Redox Reactions
- Oxidation: removal of electrons from a substrate (e- donor)
- Reduction: addition of electrons to a substrate (e- acceptor)
- Important redox rules are balancing Carbon (to CO2) and other elements, and hydrogen and oxygen atoms by using H2O or H+.
Chapter 6
- Energy is used to drive endergonic reactions
- High-energy phosphate bonds (e.g., in ATP, GTP) are crucial.
- Transmembrane ion gradients (e.g., H+, Na+) store energy.
- ATP is generated in two ways: substrate-level phosphorylation (SLP) and via transmembrane ion gradients.
Chapter 7 Biosynthesis
- Biosynthesis requires starting materials and reducing equivalents (like NADPH).
- NAD+/NADH and NADP+/NADPH play distinct roles in energy reactions and biosynthesis, respectively
- Key enzymes in biosynthetic pathways are often allosterically regulated.
- Precursor metabolites are the building blocks for biosynthesis
Chapter 8-9 Proteins
- Proteins can be modified covalently through cleavage, the formation of disulfide linkages (S–S bonds), and the addition of various molecules (e.g., phosphate, methyl groups, sugars).
- Chaperones are proteins that assist in protein folding and refolding.
- Sec system, involved in protein transport across the membranes
Chapter 17 Viruses
- Viruses are not organisms but infectious agents.
- Fundamental components include nucleic acid (DNA or RNA) and a protein coat (capsid).
- Viruses can have envelopes
- Viral replication involves several stages: attachment, penetration, uncoating, replication, assembly, and release.
- Viruses can cause disease by damaging host cells, disrupting their function, or inducing inflammatory responses.
Chapter 18 viral replication
- DNA viruses use the host's DNA polymerase to make mRNA.
- RNA viruses make RNA with the help of viral RNA replication.
- Retroviruses use reverse transcriptase to make DNA from RNA
- Viral proteins are synthesized using the host cell's ribosomes.
- Translation of mRNA produces viral proteins
- Viruses assemble new particles using the viral proteins
- New viruses are released, typically by budding or lysis.
Chapter 19, Temperate Phage
- Temperate phages can choose between the lytic and lysogenic cycle.
- The lytic cycle results in destruction of the host cell with an increase in viruses.
- The lysogenic cycle integrates viral DNA into the bacterial chromosome to produce a prophage.
Extra Cellular Matrix
- The ECM is a complex network of extracellular molecules and fibers that supports, organizes, and separates cells.
Fermentation
- Fermentation is a metabolic pathway that occurs in the absence of oxygen.
- Fermentation regenerates oxidized electron carriers.
- End products are different based on conditions
- Fermentation processes produce compounds like lactic acid and ethanol.
Nutrient Transport
- Active transport requires energy to move molecules across cell membranes from low to high concentrations.
- Passive transport does not require energy.
- Facilitated diffusion is a type of passive transport assisted by membrane proteins.
- TonB-dependent transporters are responsible for transporting many molecules, like the siderophore, across the cell membrane.
Redox Potential
- A measure of a substance's tendency to gain or lose electrons
- Higher redox potentials are better at gaining electrons
- A large difference between redox potentials means a significant release of energy in a reaction
Photosynthesis
- Photosynthesis uses light energy to convert carbon dioxide and water into glucose.
- Photosynthesis occurs in two stages: light-dependent reactions and light-independent reactions.
Enzyme Activity
- Enzymes catalyze reactions faster than they would occur spontaneously.
- Enzyme activity is typically regulated to control the rate of metabolic pathways.
- Regulation mechanisms include feedback inhibition and allosteric regulation.
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