Molecular Biology Quiz on DNA and RNA Functions
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Questions and Answers

What is the primary function of messenger RNA (mRNA)?

  • To code for proteins by carrying genetic information from DNA to ribosomes. (correct)
  • To transport amino acids to ribosomes for protein synthesis.
  • To regulate gene expression by binding to specific DNA sequences.
  • To synthesize new DNA strands during replication.
  • According to Chargaff's rules, if the percentage of guanine (G) in a DNA molecule is 25%, what is the percentage of cytosine (C) in the same molecule?

  • 10%
  • 50%
  • 25% (correct)
  • 75%
  • How did Oswald Avery's experiments contribute to the understanding of DNA as the genetic material?

  • Avery discovered the structure of DNA, revealing its double helix shape.
  • Avery identified the base pairing rules in DNA, explaining how genetic information is encoded.
  • Avery's work showed that DNA, not protein, was responsible for transforming bacteria. (correct)
  • Avery's experiments proved that RNA, not DNA, is the primary genetic material.
  • Which type of RNA is responsible for bringing specific amino acids to the ribosomes during protein synthesis?

    <p>transfer RNA (tRNA) (B)</p> Signup and view all the answers

    What makes DNA a suitable molecule for storing and transmitting genetic information?

    <p>Its ability to replicate itself accurately ensures that genetic information is passed on unchanged. (D)</p> Signup and view all the answers

    What is the main function of helix destabilizing proteins (HD proteins) in DNA replication?

    <p>Unwind the DNA double helix ahead of the replication fork (D)</p> Signup and view all the answers

    How do HD proteins differ from DNA topoisomerases?

    <p>HD proteins act only on single-stranded DNA, while topoisomerases act on double-stranded DNA. (C)</p> Signup and view all the answers

    Which of these is NOT a characteristic of HD proteins?

    <p>They act as enzymes to unwind DNA. (B)</p> Signup and view all the answers

    Why is the separation of parental DNA strands essential for replication?

    <p>To provide a template for DNA polymerase to synthesize new strands. (A)</p> Signup and view all the answers

    In what way does the replication process differ between prokaryotes and eukaryotes?

    <p>Prokaryotes have a single origin of replication, while eukaryotes have multiple origins. (B)</p> Signup and view all the answers

    What is the primary function of the 3' to 5' exonuclease activity of DNA polymerase III?

    <p>To remove mismatched nucleotides from the newly synthesized strand (A)</p> Signup and view all the answers

    What is the role of the 'replication fork' in DNA replication?

    <p>It is the region where the two parental DNA strands separate and unwind. (A)</p> Signup and view all the answers

    What is the effect of nucleotide analogues on DNA replication?

    <p>They block DNA chain growth, slowing down cell division (A)</p> Signup and view all the answers

    Why is the replication of double-stranded DNA considered 'bidirectional'?

    <p>Because replication proceeds in both directions from the origin of replication. (A)</p> Signup and view all the answers

    What is the primary function of an RNA primer in DNA replication?

    <p>To provide a starting point for DNA polymerase to synthesize new DNA strands. (D)</p> Signup and view all the answers

    What is the significance of accurate DNA replication for an organism?

    <p>All of the above. (D)</p> Signup and view all the answers

    What does the term 'deleterious mutation' refer to?

    <p>A change in the DNA sequence that adversely affects the organism's fitness. (D)</p> Signup and view all the answers

    What happens when DNA polymerase III encounters a mismatched base during replication?

    <p>It removes the mismatched base and replaces it with the correct base. (C)</p> Signup and view all the answers

    What is the role of the 'HD Protein Helicase' in DNA replication?

    <p>Unwinding the DNA double helix (B)</p> Signup and view all the answers

    In the process of DNA replication, why is one strand called the leading strand and the other called the lagging strand?

    <p>The leading strand is synthesized continuously, while the lagging strand is synthesized in fragments (Okazaki fragments). (A)</p> Signup and view all the answers

    Which of the following statements is TRUE about the 5' to 3' polymerase activity of DNA polymerase III?

    <p>It is used to add nucleotides to the growing DNA strand. (C)</p> Signup and view all the answers

    What is the primary function of the 5’→3’ exonuclease activity of DNA polymerase I?

    <p>Remove nucleotides from a base paired region (D)</p> Signup and view all the answers

    What catalyzes the final phosphodiester linkage in DNA synthesis?

    <p>DNA ligase (B)</p> Signup and view all the answers

    What activity does DNA polymerase I perform to remove RNA primers?

    <p>5'→3' exonuclease activity (C)</p> Signup and view all the answers

    Which DNA polymerase is known as RNA polymerase or DNA primase and lays down primers on the lagging strand?

    <p>DNA polymerase α (A)</p> Signup and view all the answers

    Which of the following is a role of DNA polymerase β?

    <p>Editing and repair of DNA (C)</p> Signup and view all the answers

    Which function is specifically attributed to the 3'→5' exonuclease activity of DNA polymerase?

    <p>Degrading improperly base paired nucleotides (B), Proofreading the newly synthesized DNA (D)</p> Signup and view all the answers

    What provides energy for the joining of DNA strands in humans during replication?

    <p>Cleavage of ATP to AMP and PPi (D)</p> Signup and view all the answers

    What role does DNA polymerase III play in DNA synthesis?

    <p>It synthesizes DNA until blocked by RNA primers. (B)</p> Signup and view all the answers

    What is the key difference between the 5’→3’ and 3’→5’ exonuclease activities of DNA polymerases?

    <p>5’→3’ exonuclease removes one nucleotide at a time (B)</p> Signup and view all the answers

    How does DNA polymerase I locate the gap between newly synthesized DNA and RNA primer?

    <p>Locating the nick between DNA and RNA (A)</p> Signup and view all the answers

    Which DNA polymerase is unique to mitochondria?

    <p>DNA polymerase Υ (B)</p> Signup and view all the answers

    What direction does DNA polymerase I move to remove RNA nucleotides?

    <p>5'→3' (B)</p> Signup and view all the answers

    What type of activity does DNA polymerase I have in addition to 5'→3' polymerase activity?

    <p>5'→3' exonuclease activity (C)</p> Signup and view all the answers

    What process continues until RNA is totally degraded during DNA replication?

    <p>DNA proofreading and synthesis (B)</p> Signup and view all the answers

    What does the proofreading function of DNA polymerase involve?

    <p>Detection of incorrect base pairs (D)</p> Signup and view all the answers

    What initially blocks DNA polymerase III from synthesizing DNA?

    <p>A stretch of RNA primer (B)</p> Signup and view all the answers

    What is the main difference between the leading strand and lagging strand during DNA synthesis?

    <p>The leading strand is synthesized continuously, while the lagging strand is synthesized in fragments. (C)</p> Signup and view all the answers

    What role does the primer play in DNA synthesis?

    <p>It provides the necessary initiator sequence for DNA polymerase. (A)</p> Signup and view all the answers

    Which enzyme is primarily responsible for catalyzing DNA chain elongation?

    <p>DNA polymerase III (C)</p> Signup and view all the answers

    In which direction does the new DNA strand grow during synthesis?

    <p>5'→3' (C)</p> Signup and view all the answers

    What is required for DNA polymerases to initiate synthesis?

    <p>A short double-stranded region with a free OH group (A)</p> Signup and view all the answers

    What are Okazaki fragments?

    <p>Discontinuous segments of DNA synthesized on the lagging strand. (A)</p> Signup and view all the answers

    Which of the following deoxyribonucleoside triphosphates are NOT required for DNA synthesis?

    <p>ATP (D)</p> Signup and view all the answers

    How does the nucleotide building block of DNA exist?

    <p>As deoxyribonucleoside triphosphates. (C)</p> Signup and view all the answers

    Flashcards

    Exonuclease Activity

    The process of removing nucleotides from DNA ends, either 5'→3' or 3'→5'.

    5'→3' Exonuclease

    Exonuclease activity that removes nucleotides from the 5' end of DNA.

    3'→5' Exonuclease

    Exonuclease activity that removes nucleotides from the 3' end of DNA.

    DNA Polymerase I

    Enzyme with exonuclease activity that repairs DNA and removes RNA primers.

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    DNA Polymerase III

    Main enzyme in DNA replication for synthesizing new DNA strands.

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    DNA Ligase

    Enzyme that connects DNA strands by forming phosphodiester bonds.

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    ATP Cleavage

    Process providing energy required for DNA ligation, breaking ATP into AMP and PPi.

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    DNA Polymerases in Eukaryotes

    Group of at least 15 DNA polymerases each with unique functions in DNA processes.

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    Leading strand

    The continuous strand of DNA synthesized during DNA replication.

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    Lagging strand

    The discontinuous strand of DNA synthesized in short segments during DNA replication.

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    5'→3' polymerase activity

    The activity where DNA polymerase adds nucleotides in a 5' to 3' direction during replication.

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    3'→5' exonuclease activity

    The proofreading function of DNA polymerase that removes incorrectly paired nucleotides.

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    Replication fidelity

    The accuracy of DNA replication, ensuring that the nucleotide sequence is copied correctly.

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    Nucleotide analogues

    Modified nucleotides that can block DNA replication and slow cell division.

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    Hydrolytic removal

    The process by which DNA polymerase III removes incorrectly inserted nucleotides during replication.

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    Okazaki Fragments

    Short DNA fragments synthesized on the lagging strand during replication.

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    RNA Primer

    Short RNA sequence that provides a starting point for DNA synthesis.

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    3' OH Group

    The end of a DNA or RNA strand that provides a site for nucleotide addition.

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    DNA Chain Elongation

    Process of adding nucleotides to the growing DNA strand, catalyzed by DNA polymerase.

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    Anti-Parallel Strands

    DNA strands that run in opposite directions, essential for replication.

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    Strand Separation

    The process where DNA strands separate for replication.

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    Replication Fork

    The Y-shaped region where DNA strands unwind during replication.

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    Helix Destabilizing Proteins (HD Proteins)

    Proteins that keep the DNA strands separated during replication.

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    Single-Stranded DNA Binding Proteins (SSB)

    Proteins that bind to and stabilize single-stranded DNA.

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    Replication Origin (Ori)

    The starting point for DNA replication in prokaryotes.

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    Structure of DNA

    DNA is a double-helix made of nucleotides, consisting of a sugar, phosphate group, and nitrogenous base.

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    Chargaff's Rules

    1. Percent guanine equals percent cytosine. 2) Percent thymine equals percent adenine. DNA composition varies by species.
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    Types of RNA

    RNA types include mRNA (carries codes), rRNA (part of ribosomes), and tRNA (transfers amino acids).

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    Photo 51

    An X-ray diffraction image crucial for understanding DNA structure, taken by Rosalind Franklin.

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    RNA Polymerase

    An enzyme that synthesizes RNA from a DNA template, important for transcription.

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    Nick

    The space between the 3' end of newly synthesized DNA and the 5' end of the RNA primer.

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    Study Notes

    DNA and RNA Structure

    • DNA is a double helix with two antiparallel strands
    • The strands are held together by hydrogen bonds between complementary base pairs (A-T, G-C)
    • Distance between base pairs is 0.34 nm
    • DNA has a sugar-phosphate backbone
    • RNA is a single-stranded molecule
    • RNA has a different sugar (ribose) than DNA (deoxyribose)
    • RNA contains uracil (U) instead of thymine (T)
    • Different types of RNA include mRNA, tRNA, and rRNA

    Erwin Chargaff's Rules

    • Chargaff's rules describe the base composition of DNA
    • Rule 1: The amount of guanine equals the amount of cytosine, and the amount of adenine equals the amount of thymine
    • Rule 2: The base composition of DNA varies between species

    Photo 51

    • Photo 51 is an X-ray diffraction image of DNA
    • It was taken by Rosalind Franklin and a student.

    Oswald Avery

    • Oswald Avery's work was significant in demonstrating DNA's role as the transforming molecule.
    • He experimented on bacterial extracts to determine how traits are passed down.

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    Description

    Test your knowledge on the critical roles of DNA and RNA in molecular biology. This quiz covers concepts such as mRNA, Chargaff's rules, and the functions of proteins in DNA replication. Perfect for students studying genetics and biochemistry.

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