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Questions and Answers
What is the primary function of messenger RNA (mRNA)?
What is the primary function of messenger RNA (mRNA)?
According to Chargaff's rules, if the percentage of guanine (G) in a DNA molecule is 25%, what is the percentage of cytosine (C) in the same molecule?
According to Chargaff's rules, if the percentage of guanine (G) in a DNA molecule is 25%, what is the percentage of cytosine (C) in the same molecule?
How did Oswald Avery's experiments contribute to the understanding of DNA as the genetic material?
How did Oswald Avery's experiments contribute to the understanding of DNA as the genetic material?
Which type of RNA is responsible for bringing specific amino acids to the ribosomes during protein synthesis?
Which type of RNA is responsible for bringing specific amino acids to the ribosomes during protein synthesis?
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What makes DNA a suitable molecule for storing and transmitting genetic information?
What makes DNA a suitable molecule for storing and transmitting genetic information?
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What is the main function of helix destabilizing proteins (HD proteins) in DNA replication?
What is the main function of helix destabilizing proteins (HD proteins) in DNA replication?
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How do HD proteins differ from DNA topoisomerases?
How do HD proteins differ from DNA topoisomerases?
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Which of these is NOT a characteristic of HD proteins?
Which of these is NOT a characteristic of HD proteins?
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Why is the separation of parental DNA strands essential for replication?
Why is the separation of parental DNA strands essential for replication?
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In what way does the replication process differ between prokaryotes and eukaryotes?
In what way does the replication process differ between prokaryotes and eukaryotes?
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What is the primary function of the 3' to 5' exonuclease activity of DNA polymerase III?
What is the primary function of the 3' to 5' exonuclease activity of DNA polymerase III?
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What is the role of the 'replication fork' in DNA replication?
What is the role of the 'replication fork' in DNA replication?
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What is the effect of nucleotide analogues on DNA replication?
What is the effect of nucleotide analogues on DNA replication?
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Why is the replication of double-stranded DNA considered 'bidirectional'?
Why is the replication of double-stranded DNA considered 'bidirectional'?
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What is the primary function of an RNA primer in DNA replication?
What is the primary function of an RNA primer in DNA replication?
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What is the significance of accurate DNA replication for an organism?
What is the significance of accurate DNA replication for an organism?
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What does the term 'deleterious mutation' refer to?
What does the term 'deleterious mutation' refer to?
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What happens when DNA polymerase III encounters a mismatched base during replication?
What happens when DNA polymerase III encounters a mismatched base during replication?
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What is the role of the 'HD Protein Helicase' in DNA replication?
What is the role of the 'HD Protein Helicase' in DNA replication?
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In the process of DNA replication, why is one strand called the leading strand and the other called the lagging strand?
In the process of DNA replication, why is one strand called the leading strand and the other called the lagging strand?
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Which of the following statements is TRUE about the 5' to 3' polymerase activity of DNA polymerase III?
Which of the following statements is TRUE about the 5' to 3' polymerase activity of DNA polymerase III?
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What is the primary function of the 5’→3’ exonuclease activity of DNA polymerase I?
What is the primary function of the 5’→3’ exonuclease activity of DNA polymerase I?
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What catalyzes the final phosphodiester linkage in DNA synthesis?
What catalyzes the final phosphodiester linkage in DNA synthesis?
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What activity does DNA polymerase I perform to remove RNA primers?
What activity does DNA polymerase I perform to remove RNA primers?
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Which DNA polymerase is known as RNA polymerase or DNA primase and lays down primers on the lagging strand?
Which DNA polymerase is known as RNA polymerase or DNA primase and lays down primers on the lagging strand?
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Which of the following is a role of DNA polymerase β?
Which of the following is a role of DNA polymerase β?
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Which function is specifically attributed to the 3'→5' exonuclease activity of DNA polymerase?
Which function is specifically attributed to the 3'→5' exonuclease activity of DNA polymerase?
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What provides energy for the joining of DNA strands in humans during replication?
What provides energy for the joining of DNA strands in humans during replication?
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What role does DNA polymerase III play in DNA synthesis?
What role does DNA polymerase III play in DNA synthesis?
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What is the key difference between the 5’→3’ and 3’→5’ exonuclease activities of DNA polymerases?
What is the key difference between the 5’→3’ and 3’→5’ exonuclease activities of DNA polymerases?
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How does DNA polymerase I locate the gap between newly synthesized DNA and RNA primer?
How does DNA polymerase I locate the gap between newly synthesized DNA and RNA primer?
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Which DNA polymerase is unique to mitochondria?
Which DNA polymerase is unique to mitochondria?
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What direction does DNA polymerase I move to remove RNA nucleotides?
What direction does DNA polymerase I move to remove RNA nucleotides?
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What type of activity does DNA polymerase I have in addition to 5'→3' polymerase activity?
What type of activity does DNA polymerase I have in addition to 5'→3' polymerase activity?
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What process continues until RNA is totally degraded during DNA replication?
What process continues until RNA is totally degraded during DNA replication?
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What does the proofreading function of DNA polymerase involve?
What does the proofreading function of DNA polymerase involve?
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What initially blocks DNA polymerase III from synthesizing DNA?
What initially blocks DNA polymerase III from synthesizing DNA?
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What is the main difference between the leading strand and lagging strand during DNA synthesis?
What is the main difference between the leading strand and lagging strand during DNA synthesis?
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What role does the primer play in DNA synthesis?
What role does the primer play in DNA synthesis?
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Which enzyme is primarily responsible for catalyzing DNA chain elongation?
Which enzyme is primarily responsible for catalyzing DNA chain elongation?
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In which direction does the new DNA strand grow during synthesis?
In which direction does the new DNA strand grow during synthesis?
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What is required for DNA polymerases to initiate synthesis?
What is required for DNA polymerases to initiate synthesis?
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What are Okazaki fragments?
What are Okazaki fragments?
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Which of the following deoxyribonucleoside triphosphates are NOT required for DNA synthesis?
Which of the following deoxyribonucleoside triphosphates are NOT required for DNA synthesis?
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How does the nucleotide building block of DNA exist?
How does the nucleotide building block of DNA exist?
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Flashcards
Exonuclease Activity
Exonuclease Activity
The process of removing nucleotides from DNA ends, either 5'→3' or 3'→5'.
5'→3' Exonuclease
5'→3' Exonuclease
Exonuclease activity that removes nucleotides from the 5' end of DNA.
3'→5' Exonuclease
3'→5' Exonuclease
Exonuclease activity that removes nucleotides from the 3' end of DNA.
DNA Polymerase I
DNA Polymerase I
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DNA Polymerase III
DNA Polymerase III
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DNA Ligase
DNA Ligase
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ATP Cleavage
ATP Cleavage
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DNA Polymerases in Eukaryotes
DNA Polymerases in Eukaryotes
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Leading strand
Leading strand
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Lagging strand
Lagging strand
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5'→3' polymerase activity
5'→3' polymerase activity
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3'→5' exonuclease activity
3'→5' exonuclease activity
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Replication fidelity
Replication fidelity
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Nucleotide analogues
Nucleotide analogues
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Hydrolytic removal
Hydrolytic removal
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Okazaki Fragments
Okazaki Fragments
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RNA Primer
RNA Primer
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3' OH Group
3' OH Group
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DNA Chain Elongation
DNA Chain Elongation
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Anti-Parallel Strands
Anti-Parallel Strands
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Strand Separation
Strand Separation
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Replication Fork
Replication Fork
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Helix Destabilizing Proteins (HD Proteins)
Helix Destabilizing Proteins (HD Proteins)
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Single-Stranded DNA Binding Proteins (SSB)
Single-Stranded DNA Binding Proteins (SSB)
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Replication Origin (Ori)
Replication Origin (Ori)
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Structure of DNA
Structure of DNA
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Chargaff's Rules
Chargaff's Rules
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Types of RNA
Types of RNA
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Photo 51
Photo 51
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RNA Polymerase
RNA Polymerase
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Nick
Nick
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Study Notes
DNA and RNA Structure
- DNA is a double helix with two antiparallel strands
- The strands are held together by hydrogen bonds between complementary base pairs (A-T, G-C)
- Distance between base pairs is 0.34 nm
- DNA has a sugar-phosphate backbone
- RNA is a single-stranded molecule
- RNA has a different sugar (ribose) than DNA (deoxyribose)
- RNA contains uracil (U) instead of thymine (T)
- Different types of RNA include mRNA, tRNA, and rRNA
Erwin Chargaff's Rules
- Chargaff's rules describe the base composition of DNA
- Rule 1: The amount of guanine equals the amount of cytosine, and the amount of adenine equals the amount of thymine
- Rule 2: The base composition of DNA varies between species
Photo 51
- Photo 51 is an X-ray diffraction image of DNA
- It was taken by Rosalind Franklin and a student.
Oswald Avery
- Oswald Avery's work was significant in demonstrating DNA's role as the transforming molecule.
- He experimented on bacterial extracts to determine how traits are passed down.
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Description
Test your knowledge on the critical roles of DNA and RNA in molecular biology. This quiz covers concepts such as mRNA, Chargaff's rules, and the functions of proteins in DNA replication. Perfect for students studying genetics and biochemistry.