18 Questions
What technique involves fixing DNA to a substrate and then hybridizing a DNA probe to the fixed DNA?
Southern Blot
Trp is best described as an ______ effector.
Allosteric
Which of the following is best described as a “cis-acting” element?
cAMP response element (CRE)
How many genes encode insulin?
One
Which enzyme synthesizes three eukaryotic rRNAs?
RNA polymerase 1
What natural cellular process requires reverse transcription?
Replication of chromosome ends
What other method can be used to amplify the number of copies of a particular sequence?
Polymerase Chain reaction
What is the function of Titin in muscle?
Acting as a molecular spring
What is the main function of SDS and B-mercaptoethanol in protein treatment?
Separating protein subunits
Which cellular process requires ligation?
Replication
What is the effect of gastrin on stomach pH and of secretin on intestinal pH?
Gastrin decreases stomach pH and Secretin increases intestinal pH
Which two amino acids can’t be used directly for Gluconeogenesis?
Leucine, Lysine
Where else have you seen aspartate?
In a shuttle that transfers reducing equivalents from cytosol to mitochondria
How is NADPH made and what is it also used for?
In the PPP; used for fatty acid synthesis and cholesterol synthesis
Which of the following enzymes incorporates ammonia into a non-toxic amino acid for storage and circulatory transport?
Glutamine synthetase
Which of these compounds always contain Nitrogen?
Amino Acids
Which is an obligatory, allosteric activator of CPS1?
N-acetyl glutamate (NAG)
A deficiency in catabolism of this monosaccharide causes a gain of function pathology:
Galactose
Test your knowledge of molecular biology with this quiz covering topics such as the encoding of serine triplets, the synthesis of eukaryotic rRNAs, the translation rate of ribosomes, and the nature of enzymes.
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