Podcast
Questions and Answers
If you have a strain of bacteria that produce nonfunctional sigma factor, which step of transcription would you expect will be affected?
If you have a strain of bacteria that produce nonfunctional sigma factor, which step of transcription would you expect will be affected?
- These bacteria will produce defective mRNA.
- These bacteria will be unable to assemble RNA polymerase onto the DNA strand. (correct)
- These bacteria will have an increased rate of transcription.
- These bacteria will be unable to terminate transcription.
Which of the following is not required for the expression of genes in the lactose operon?
Which of the following is not required for the expression of genes in the lactose operon?
- RNA polymerase
- Lactose
- Promoter
- Adenylate cyclase (correct)
Which of the following statements is not correct?
Which of the following statements is not correct?
- Bacteria have multiple sigma factors.
- Sigma factors are involved in promoter recognition.
- RNA polymerase is essential for transcription.
- Bacteria possess only one type of RNA polymerase. (correct)
What does the CAP protein stand for?
What does the CAP protein stand for?
Which sigma factor is highly heat stable?
Which sigma factor is highly heat stable?
Gene imprinting involves:
Gene imprinting involves:
Which of the following sequences can undergo methylation?
Which of the following sequences can undergo methylation?
What is the functional product of the Xist gene?
What is the functional product of the Xist gene?
What is the primary function of the CAP protein?
What is the primary function of the CAP protein?
Which type of RNA polymerase is found in bacteria?
Which type of RNA polymerase is found in bacteria?
Which of the following statements about enhancers is false?
Which of the following statements about enhancers is false?
Which of the following processes is involved in gene imprinting?
Which of the following processes is involved in gene imprinting?
Flashcards
Sigma factor
Sigma factor
A protein essential for the assembly of RNA polymerase onto DNA, enabling transcription initiation.
Operon
Operon
A specific region on a bacterial chromosome that contains genes related to the metabolism of a particular substance, like lactose.
CAP protein (Catabolite activator protein)
CAP protein (Catabolite activator protein)
A regulatory protein that binds to DNA upstream of the promoter of the lac operon, enhancing transcription when lactose is present.
σ32
σ32
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Gene imprinting
Gene imprinting
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Methylation sites
Methylation sites
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AGGTTC
AGGTTC
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Xist RNA
Xist RNA
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CAP protein's function
CAP protein's function
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Bacterial RNA Polymerase
Bacterial RNA Polymerase
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Enhancers
Enhancers
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DNA methylation
DNA methylation
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Study Notes
Transcription in Bacteria
- A strain of bacteria with a nonfunctional sigma factor would affect the assembly of RNA polymerase onto the DNA strand.
Lactose Operon
- Adenylate cyclase is not required for the expression of genes in the lactose operon.
RNA Polymerase in Bacteria
- Bacteria possess only one type of RNA polymerase.
CAP (Catabolite Gene Activator Protein)
- CAP is a catabolite gene activator protein.
Sigma Factors
- Four types of sigma factors are known, and σ32 is highly heat stable.
Enhancers
- Enhancers do not work as both cis and trans-acting sequences.
Gene Imprinting
- Gene imprinting involves DNA methylation.
Methylation in Oligonucleotides
- Methylation is possible within the oligonucleotides at the bases AGGTTC.
Xist Gene
- The functional product of the Xist gene is lncRNA (long non-coding RNA).
Transcription in Bacteria
- A strain of bacteria with a nonfunctional sigma factor would affect the assembly of RNA polymerase onto the DNA strand.
Lactose Operon
- Adenylate cyclase is not required for the expression of genes in the lactose operon.
RNA Polymerase in Bacteria
- Bacteria possess only one type of RNA polymerase.
CAP (Catabolite Gene Activator Protein)
- CAP is a catabolite gene activator protein.
Sigma Factors
- Four types of sigma factors are known, and σ32 is highly heat stable.
Enhancers
- Enhancers do not work as both cis and trans-acting sequences.
Gene Imprinting
- Gene imprinting involves DNA methylation.
Methylation in Oligonucleotides
- Methylation is possible within the oligonucleotides at the bases AGGTTC.
Xist Gene
- The functional product of the Xist gene is lncRNA (long non-coding RNA).
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