Molecular Biology PhD Entrance Exam 2023-2024
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Questions and Answers

If you have a strain of bacteria that produce nonfunctional sigma factor, which step of transcription would you expect will be affected?

  • These bacteria will produce defective mRNA.
  • These bacteria will be unable to assemble RNA polymerase onto the DNA strand. (correct)
  • These bacteria will have an increased rate of transcription.
  • These bacteria will be unable to terminate transcription.
  • Which of the following is not required for the expression of genes in the lactose operon?

  • RNA polymerase
  • Lactose
  • Promoter
  • Adenylate cyclase (correct)
  • Which of the following statements is not correct?

  • Bacteria have multiple sigma factors.
  • Sigma factors are involved in promoter recognition.
  • RNA polymerase is essential for transcription.
  • Bacteria possess only one type of RNA polymerase. (correct)
  • What does the CAP protein stand for?

    <p>Catabolite gene activator protein.</p> Signup and view all the answers

    Which sigma factor is highly heat stable?

    <p>σ32</p> Signup and view all the answers

    Gene imprinting involves:

    <p>DNA methylation</p> Signup and view all the answers

    Which of the following sequences can undergo methylation?

    <p>AGGTTC</p> Signup and view all the answers

    What is the functional product of the Xist gene?

    <p>lncRNA</p> Signup and view all the answers

    What is the primary function of the CAP protein?

    <p>To activate gene transcription</p> Signup and view all the answers

    Which type of RNA polymerase is found in bacteria?

    <p>Only one type of RNA polymerase</p> Signup and view all the answers

    Which of the following statements about enhancers is false?

    <p>They work as both cis and trans-acting sequences.</p> Signup and view all the answers

    Which of the following processes is involved in gene imprinting?

    <p>DNA methylation</p> Signup and view all the answers

    Study Notes

    Transcription in Bacteria

    • A strain of bacteria with a nonfunctional sigma factor would affect the assembly of RNA polymerase onto the DNA strand.

    Lactose Operon

    • Adenylate cyclase is not required for the expression of genes in the lactose operon.

    RNA Polymerase in Bacteria

    • Bacteria possess only one type of RNA polymerase.

    CAP (Catabolite Gene Activator Protein)

    • CAP is a catabolite gene activator protein.

    Sigma Factors

    • Four types of sigma factors are known, and σ32 is highly heat stable.

    Enhancers

    • Enhancers do not work as both cis and trans-acting sequences.

    Gene Imprinting

    • Gene imprinting involves DNA methylation.

    Methylation in Oligonucleotides

    • Methylation is possible within the oligonucleotides at the bases AGGTTC.

    Xist Gene

    • The functional product of the Xist gene is lncRNA (long non-coding RNA).

    Transcription in Bacteria

    • A strain of bacteria with a nonfunctional sigma factor would affect the assembly of RNA polymerase onto the DNA strand.

    Lactose Operon

    • Adenylate cyclase is not required for the expression of genes in the lactose operon.

    RNA Polymerase in Bacteria

    • Bacteria possess only one type of RNA polymerase.

    CAP (Catabolite Gene Activator Protein)

    • CAP is a catabolite gene activator protein.

    Sigma Factors

    • Four types of sigma factors are known, and σ32 is highly heat stable.

    Enhancers

    • Enhancers do not work as both cis and trans-acting sequences.

    Gene Imprinting

    • Gene imprinting involves DNA methylation.

    Methylation in Oligonucleotides

    • Methylation is possible within the oligonucleotides at the bases AGGTTC.

    Xist Gene

    • The functional product of the Xist gene is lncRNA (long non-coding RNA).

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    Description

    Practice questions for PhD students on molecular biology, including transcription and gene expression. Test your knowledge on sigma factors, RNA polymerase, and lactose operon.

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