12 Questions
If you have a strain of bacteria that produce nonfunctional sigma factor, which step of transcription would you expect will be affected?
These bacteria will be unable to assemble RNA polymerase onto the DNA strand.
Which of the following is not required for the expression of genes in the lactose operon?
Adenylate cyclase
Which of the following statements is not correct?
Bacteria possess only one type of RNA polymerase.
What does the CAP protein stand for?
Catabolite gene activator protein.
Which sigma factor is highly heat stable?
σ32
Gene imprinting involves:
DNA methylation
Which of the following sequences can undergo methylation?
AGGTTC
What is the functional product of the Xist gene?
lncRNA
What is the primary function of the CAP protein?
To activate gene transcription
Which type of RNA polymerase is found in bacteria?
Only one type of RNA polymerase
Which of the following statements about enhancers is false?
They work as both cis and trans-acting sequences.
Which of the following processes is involved in gene imprinting?
DNA methylation
Study Notes
Transcription in Bacteria
- A strain of bacteria with a nonfunctional sigma factor would affect the assembly of RNA polymerase onto the DNA strand.
Lactose Operon
- Adenylate cyclase is not required for the expression of genes in the lactose operon.
RNA Polymerase in Bacteria
- Bacteria possess only one type of RNA polymerase.
CAP (Catabolite Gene Activator Protein)
- CAP is a catabolite gene activator protein.
Sigma Factors
- Four types of sigma factors are known, and σ32 is highly heat stable.
Enhancers
- Enhancers do not work as both cis and trans-acting sequences.
Gene Imprinting
- Gene imprinting involves DNA methylation.
Methylation in Oligonucleotides
- Methylation is possible within the oligonucleotides at the bases AGGTTC.
Xist Gene
- The functional product of the Xist gene is lncRNA (long non-coding RNA).
Transcription in Bacteria
- A strain of bacteria with a nonfunctional sigma factor would affect the assembly of RNA polymerase onto the DNA strand.
Lactose Operon
- Adenylate cyclase is not required for the expression of genes in the lactose operon.
RNA Polymerase in Bacteria
- Bacteria possess only one type of RNA polymerase.
CAP (Catabolite Gene Activator Protein)
- CAP is a catabolite gene activator protein.
Sigma Factors
- Four types of sigma factors are known, and σ32 is highly heat stable.
Enhancers
- Enhancers do not work as both cis and trans-acting sequences.
Gene Imprinting
- Gene imprinting involves DNA methylation.
Methylation in Oligonucleotides
- Methylation is possible within the oligonucleotides at the bases AGGTTC.
Xist Gene
- The functional product of the Xist gene is lncRNA (long non-coding RNA).
Practice questions for PhD students on molecular biology, including transcription and gene expression. Test your knowledge on sigma factors, RNA polymerase, and lactose operon.
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