Podcast
Questions and Answers
What is the primary function of a multiple cloning site in a plasmid?
What is the primary function of a multiple cloning site in a plasmid?
- To provide recognition sites for several restriction enzymes (correct)
- To increase the copy number of the plasmid
- To mediate DNA transfer through conjugation
- To allow for the selection of transformed colonies
What is the purpose of a unique restriction enzyme cleavage site in a plasmid?
What is the purpose of a unique restriction enzyme cleavage site in a plasmid?
- To mediate DNA transfer through conjugation
- To provide a site for DNA sequencing
- To confer antibiotic resistance to the host cell
- To allow for the splicing of DNA sequences into the plasmid (correct)
What is the function of restriction enzymes in plasmid cloning?
What is the function of restriction enzymes in plasmid cloning?
- To ligate DNA fragments into the plasmid
- To mediate DNA transfer through conjugation
- To confer antibiotic resistance to the host cell
- To cut the plasmid DNA at specific recognition sites (correct)
What is the purpose of using restriction enzymes with different recognition sites in plasmid cloning?
What is the purpose of using restriction enzymes with different recognition sites in plasmid cloning?
What is the advantage of using plasmids with multiple cloning sites?
What is the advantage of using plasmids with multiple cloning sites?
What is the role of restriction enzymes in creating a recombinant plasmid?
What is the role of restriction enzymes in creating a recombinant plasmid?
What is the purpose of using a unique restriction enzyme cleavage site in a plasmid?
What is the purpose of using a unique restriction enzyme cleavage site in a plasmid?
What is the function of a multiple cloning site in a plasmid with respect to restriction enzymes?
What is the function of a multiple cloning site in a plasmid with respect to restriction enzymes?
What is the advantage of using restriction enzymes with different recognition sites in plasmid cloning?
What is the advantage of using restriction enzymes with different recognition sites in plasmid cloning?
What is the purpose of using restriction enzymes in plasmid cloning?
What is the purpose of using restriction enzymes in plasmid cloning?
