Molecular Biology Overview Quiz
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Questions and Answers

Which cellular molecules are primarily studied in molecular biology?

  • Vitamins and minerals
  • Water and salts
  • Carbohydrates and lipids
  • Nucleic acids and proteins (correct)
  • Before the discovery of DNA's structure, what significant contribution did Gregor Mendel make to the field of biology?

  • He identified the chemical components of DNA.
  • He discovered the basic principles of heredity. (correct)
  • He developed the first methods for DNA sequencing.
  • He cloned the first gene.
  • The cracking of the genetic code and the understanding that DNA directs protein synthesis occurred in what period?

  • Simultaneously with Mendel’s work.
  • Two decades following the discovery of DNA structure (correct)
  • Decades preceding the discovery of DNA structure
  • In 2001 with the sequencing of the human genome.
  • What major technical advancement significantly accelerated progress in molecular biology?

    <p>DNA sequencing (B)</p> Signup and view all the answers

    What was a significant achievement in molecular biology completed in 2001?

    <p>The sequencing of the human genome. (B)</p> Signup and view all the answers

    What is the anticipated focus of molecular biology in the 50 years after 2001?

    <p>Realising the practical uses of DNA discoveries in areas like industry and medicine. (C)</p> Signup and view all the answers

    The discovery of the DNA structure marked the beginning of which of the following?

    <p>A new era in biology. (C)</p> Signup and view all the answers

    Who is credited with first identifying and isolating 'nuclein' from cell nuclei?

    <p>Friedrich Miescher (D)</p> Signup and view all the answers

    Which scientist demonstrated a 'transforming principle' during the study of pneumonia in mice?

    <p>Frederick Griffith (D)</p> Signup and view all the answers

    What is Phoebus Aaron Levene's primary contribution to the understanding of DNA?

    <p>Characterizing and naming ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) (B)</p> Signup and view all the answers

    In what year was recombinant DNA technology developed?

    <p>1970 (D)</p> Signup and view all the answers

    Who is credited with the development of a method for DNA sequencing?

    <p>Frederick Sanger (C)</p> Signup and view all the answers

    What is Kary Mullis known for?

    <p>Developing In Vitro Amplification of DNA (PCR) (D)</p> Signup and view all the answers

    What was the primary goal of the Human Genome Project (HGP)?

    <p>Sequencing the entire human genome (A)</p> Signup and view all the answers

    Which scientists are most associated with deducing the double helix conformation of DNA?

    <p>Watson and Crick (B)</p> Signup and view all the answers

    In Mendel's experiments with pea plants, what characteristic was investigated in the P generation?

    <p>True-breeding flower color. (C)</p> Signup and view all the answers

    In Mendel's experiment, what proportion of the F2 generation displayed white flowers when the F1 generation hybrids all had violet flowers?

    <p>Approximately one-quarter of the plants (C)</p> Signup and view all the answers

    What does Mendel's Law of Segregation state regarding alleles?

    <p>Individuals possess two alleles, and parents pass only one allele to their offspring. (D)</p> Signup and view all the answers

    What is the term for an individual with two identical alleles for a particular trait?

    <p>Homozygous (D)</p> Signup and view all the answers

    Which of the following factors determines an individual's phenotype?

    <p>Both its alleles and environment (B)</p> Signup and view all the answers

    What was Frederick Griffith primarily trying to achieve with his experiments?

    <p>Develop a vaccine against pneumonia (A)</p> Signup and view all the answers

    What was the effect of injecting mice with live R bacteria in Griffith's experiment?

    <p>The mice remained healthy (D)</p> Signup and view all the answers

    What was the outcome when Griffith injected mice with heat-killed S bacteria?

    <p>The mice remained healthy. (C)</p> Signup and view all the answers

    What happened when Griffith injected mice with a combination of heat-killed S bacteria and live R bacteria?

    <p>The mice died. (C)</p> Signup and view all the answers

    What did Griffith conclude based on the results of his experiment?

    <p>There was a transfer of some component from the S bacteria to the R bacteria. (C)</p> Signup and view all the answers

    Why did the S strain bacteria cause pneumonia in mice, according to the information provided?

    <p>The S strain covered itself with a polysaccharide capsule, protecting it from the host's immune system. (B)</p> Signup and view all the answers

    Which characteristic distinguishes bacteria as an optimal model organism in molecular biology?

    <p>Simple growth requirements and rapid reproduction rate. (A)</p> Signup and view all the answers

    How do bacteriophages contribute to genetic research differently from bacteria?

    <p>They introduce genetic material into bacteria, acting as cloning vectors. (D)</p> Signup and view all the answers

    Why is yeast considered a valuable model organism for eukaryotic cell studies?

    <p>Its fermentation processes and easy-to-study mutant strains aid in researching cell behavior. (D)</p> Signup and view all the answers

    What role did the Human Genome Project (HGP) play in advancing biological research and medicine?

    <p>It sequenced the entire human genome that led to the rise of bioinformatics and aided understanding of genetic disorders. (A)</p> Signup and view all the answers

    Which of the following was a primary goal of the Human Genome Project?

    <p>Creating databases to store and manage human genome sequence data. (D)</p> Signup and view all the answers

    Which of the following describes a crucial difference between DNA and protein that Hershey and Chase exploited in their experiments?

    <p>DNA contains phosphorus, while protein contains sulfur. (C)</p> Signup and view all the answers

    What was the purpose of using a high-speed blender in the Hershey-Chase experiment?

    <p>To shear the viral protein shells off infecting cells. (B)</p> Signup and view all the answers

    In the Hershey-Chase experiment, where was the radioactive $^{35}$S tracer primarily located after centrifugation?

    <p>In the supernatant containing the viral protein shells. (B)</p> Signup and view all the answers

    What observation led Hershey and Chase to conclude that DNA, rather than protein, is the genetic material of bacteriophage T2?

    <p>The transfer of radioactive phosphorus into the bacterial cytoplasm. (D)</p> Signup and view all the answers

    What is the role of bacteriophage T2 in the Hershey-Chase experiment?

    <p>To act as a vector for introducing genetic material into bacteria. (A)</p> Signup and view all the answers

    Which of the following elements is present in DNA but not in any of the 20 amino acids that make up proteins?

    <p>Phosphorus (B)</p> Signup and view all the answers

    What was the purpose of using radioisotopes in the Hershey-Chase experiment?

    <p>Enable the visualization and tracking of DNA and proteins. (D)</p> Signup and view all the answers

    Following the infection of E. coli by phage and subsequent lysis, what was observed in the progeny phage particles?

    <p>They contained only $^{32}$P. (A)</p> Signup and view all the answers

    Before the Hershey-Chase experiments, what was the prevailing scientific view regarding the nature of genetic material?

    <p>Proteins were the most likely candidates for carrying genetic information. (D)</p> Signup and view all the answers

    Which of the following steps came last in the Hershey-Chase experiment?

    <p>Lysis of the bacteria (C)</p> Signup and view all the answers

    Which of the following scientists is credited with first describing the double helix structure of DNA?

    <p>Watson and Crick (A)</p> Signup and view all the answers

    What was the significance of Frederick Griffith's experiment in 1928?

    <p>It demonstrated a heritable 'transforming principle'. (D)</p> Signup and view all the answers

    What discovery is attributed to Kary Mullis?

    <p>In Vitro Amplification of DNA (PCR) (D)</p> Signup and view all the answers

    Who isolated "nuclein" from cell nuclei?

    <p>Friedrich Miescher (D)</p> Signup and view all the answers

    What term did Phoebus Aaron Levene use to describe the structure of DNA?

    <p>Tetra nucleotide (C)</p> Signup and view all the answers

    What significant contribution did Paul Berg make to the field of molecular biology?

    <p>He developed recombinant DNA technology. (A)</p> Signup and view all the answers

    What was the primary objective of the Human Genome Project (HGP)?

    <p>To map the entire human genome (C)</p> Signup and view all the answers

    Which development allowed scientists to determine the precise sequence of nucleotides in DNA molecules?

    <p>DNA sequencing (C)</p> Signup and view all the answers

    Who published a paper on inheritance of traits in peas?

    <p>Gregor Mendel (A)</p> Signup and view all the answers

    What distinguishes molecular biology from other fields of biology?

    <p>Its emphasis on the study of cellular molecules and their interactions. (B)</p> Signup and view all the answers

    What impact did the discovery of DNA structure have on the field of biology?

    <p>It inaugurated a new era in biology, leading to understanding of the genetic code and protein synthesis. (A)</p> Signup and view all the answers

    What is the long-term expectation for the practical application of the discoveries in molecular biology, such as Crick and Watson’s discovery ?

    <p>Realizing the impact of the discovery for humanity across areas like medicine, industry, and agriculture. (D)</p> Signup and view all the answers

    How did the rediscovery of Mendel’s work impact the development of molecular biology?

    <p>It led to scientists quickly understanding that genes encode life's information. (A)</p> Signup and view all the answers

    Which of the following best describes the relationship between DNA and protein synthesis?

    <p>DNA directs the synthesis of proteins. (A)</p> Signup and view all the answers

    Why are DNA sequencing, genetic engineering, and gene cloning considered significant advancements in molecular biology?

    <p>They provided tools to solve complete genomes and improve the lives of humans. (C)</p> Signup and view all the answers

    What makes DNA such an important molecule for the life and maintenance of a cell?

    <p>It directs the synthesis of proteins. (B)</p> Signup and view all the answers

    What is a primary characteristic that distinguishes E. coli as a model organism in molecular biology?

    <p>It can replicate DNA and synthesize proteins rapidly. (C)</p> Signup and view all the answers

    What method was used in the Human Genome Project to differentiate between genes and expressed RNAs?

    <p>Expressed sequence tags. (D)</p> Signup and view all the answers

    Which of the following accurately describes a bacteriophage?

    <p>It functions as a cloning vector in genetic engineering. (A)</p> Signup and view all the answers

    Which goal did the Human Genome Project aim to achieve?

    <p>To optimize data analysis from genetic sequences. (B)</p> Signup and view all the answers

    What is a primary reason yeast is used as a model organism in molecular biology?

    <p>It allows researchers to study fermentation processes. (A)</p> Signup and view all the answers

    What crucial chemical difference between DNA and protein did Hershey and Chase utilize in their experiments?

    <p>DNA contains phosphorus whereas protein does not. (B)</p> Signup and view all the answers

    What was the outcome when phages labeled with radioactive Sulfur-35 infected E. coli?

    <p>The radioactive label was found only in the viral protein shells. (A)</p> Signup and view all the answers

    What method did Hershey and Chase use to separate the phage protein coats from the infected bacterial cells?

    <p>High-speed centrifugation. (A)</p> Signup and view all the answers

    What radioactive isotope was used to label the DNA in the Hershey-Chase experiments?

    <p>Phosphorus-32. (C)</p> Signup and view all the answers

    Which conclusion did Hershey and Chase reach regarding the genetic material of bacteriophage T2?

    <p>DNA is the genetic material that initiates infection. (C)</p> Signup and view all the answers

    What was a primary aim of the Hershey-Chase experiments?

    <p>To identify the genetic material in a virus. (A)</p> Signup and view all the answers

    What significant observation was made after the lysis of E. coli infected with phage DNA?

    <p>New phage particles contained only phosphorus-32. (A)</p> Signup and view all the answers

    Which element is found in the structure of proteins but not in DNA, as used in the Hershey-Chase experiments?

    <p>Sulfur. (D)</p> Signup and view all the answers

    What technique was used to observe the transfer of 32P-labeled DNA into the host bacterium's cytoplasm?

    <p>Autoradiography. (B)</p> Signup and view all the answers

    What aspect of bacteriophage T2 did the Hershey-Chase experiments ultimately demonstrate?

    <p>It injects DNA into host cells. (C)</p> Signup and view all the answers

    What does it mean for an individual to be heterozygous for a trait?

    <p>The individual has one allele from each parent that are different. (C)</p> Signup and view all the answers

    Which observation was a key result from Mendel's F1 generation experiments with pea plants?

    <p>All plants produced violet flowers. (A)</p> Signup and view all the answers

    What was the outcome when Griffith injected mice with a mixture of live R bacteria and heat-killed S bacteria?

    <p>The mice died of pneumonia. (A)</p> Signup and view all the answers

    What is the primary significance of Griffith's finding known as the 'transforming principle'?

    <p>It showed that R bacteria could be converted into S bacteria. (D)</p> Signup and view all the answers

    According to Mendel's Law of Segregation, what occurs during the formation of gametes?

    <p>Only one of the two alleles from each parent is passed on. (D)</p> Signup and view all the answers

    What trait was specifically evident in the F2 generation of Mendel's experiments?

    <p>Three-quarters of plants exhibited violet flowers. (A)</p> Signup and view all the answers

    What was the key component that protected the S strain bacteria from the host's immune system?

    <p>A polysaccharide capsule. (D)</p> Signup and view all the answers

    Mendel defined an individual's genotype as what?

    <p>The complete set of alleles an individual possesses. (D)</p> Signup and view all the answers

    How did Griffith's experiments contribute to the understanding of genetic material?

    <p>They suggested that a 'transforming principle' could carry genetic information. (B)</p> Signup and view all the answers

    Which statement best describes the outcome of injecting mice with live S strain bacteria?

    <p>All injected mice died of pneumonia. (C)</p> Signup and view all the answers

    Flashcards

    Molecular Biology

    The study of cellular molecules and their interactions that are essential for cell functions.

    DNA Structure Discovery

    The event that marked a new era in biology, revealing DNA as the genetic material.

    Genetic Code

    The set of rules by which information encoded in DNA is translated into proteins.

    Gregor Mendel

    The scientist known as the father of genetics for his work on heredity before DNA was discovered.

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    Gene Cloning

    A method for copying a gene to produce identical copies for study or use.

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    Human Genome Project

    The international scientific research project that sequenced the complete human genome by 2001.

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    Technical Advances in Molecular Biology

    Innovations such as DNA sequencing and genetic engineering that have propelled the field forward.

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    Inheritance

    The process through which traits are passed from parents to offspring.

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    Nuclein

    The acidic substance identified by Miescher, later known as DNA.

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    Transforming Principle

    The concept that some material can change the traits of organisms, demonstrated by Griffith.

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    DNA

    The molecule that carries genetic information in living organisms.

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    Double Helix

    The shape of DNA, resembling a twisted ladder.

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    Recombinant DNA Technology

    A method to combine DNA from different sources.

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    DNA Sequencing

    The process of determining the order of nucleotides in DNA.

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    Polymerase Chain Reaction (PCR)

    A technique to amplify small segments of DNA.

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    Bacteria

    Prokaryotic, unicellular organisms with a nucleoid region and one chromosome.

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    Bacteriophage

    Viruses that infect bacteria and use the host's machinery to replicate.

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    Yeast

    Eukaryotic unicellular organisms, used in biochemical research and fermentation.

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    Human Genome Project (HGP)

    International project that sequenced the human genome, completed in 2003.

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    Expressed Sequence Tags

    Short sequences of DNA used in the HGP to identify genes and their expression.

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    Hershey-Chase Experiments

    Experiments that demonstrated DNA is the genetic material of T2 bacteriophage.

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    Bacteriophage T2

    A virus that infects bacteria, specifically E. coli.

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    Radioisotope Use

    Use of radioactive isotopes to trace DNA and protein in experiments.

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    Phosphorus-32

    Radioactive isotope used to label DNA in Hershey-Chase experiments.

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    Sulfur-35

    Radioactive isotope used to label proteins in experiments.

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    Transfer of DNA

    The process where DNA from phages enters the host bacterial cell.

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    Centrifugation

    Technique used to separate components based on density after infection.

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    E. coli Infection

    Process by which T2 bacteriophage injects DNA into E. coli bacteria.

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    Progeny Phage

    New phage particles formed from the infected host's DNA post-infection.

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    Separation of Virus and Bacteria

    Isolating viral components from host cells after infection.

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    F1 Generation

    The first generation of offspring from a genetic cross, exhibiting traits inherited from both parents.

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    F2 Generation

    The second generation of offspring, produced from crossing F1 individuals, showing a 3:1 trait ratio.

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    Law of Segregation

    Mendel's rule stating that individuals have two alleles for each trait and only one is passed to offspring.

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    Homozygous

    An individual with two identical alleles for a particular trait.

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    Heterozygous

    An individual with two different alleles for a specific trait.

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    Genotype

    The genetic constitution of an individual represented by its alleles.

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    Phenotype

    The observable physical appearance of an organism determined by its genotype and environment.

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    Frederick Griffith

    A British bacteriologist known for discovering the transforming principle in bacteria.

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    Streptococcus pneumoniae

    A type of bacteria used by Griffith in his experiments, known for causing pneumonia.

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    Cellular Molecules

    The molecules, such as DNA and proteins, that interact to support cell functions.

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    Historical Significance of DNA

    DNA's discovery transformed biological science and understanding of genetics.

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    Cracking the Genetic Code

    The process of deciphering how DNA translates into proteins.

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    Technical Advances

    Innovations that have advanced molecular biology, like DNA sequencing.

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    Human Genome Sequencing

    The complete mapping of the human genome completed in 2001.

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    Importance of Crick and Watson

    Their discovery of DNA structure laid the foundation for modern genetics.

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    Future Applications of DNA Research

    Potential benefits of DNA discoveries in various fields like medicine and agriculture.

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    Mendel’s Pea Plants

    Mendel crossed true-breeding violet and white flowers to study inheritance.

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    Tetranucleotide Structure

    Levene characterized DNA's structure; 4 bases arranged in sets.

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    Mendel's First Experiment

    Gregor Mendel's initial genetic testing with pea plants, focusing on flower color.

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    Griffith's Experiment

    An experiment demonstrating that heat-killed bacteria can transform live bacteria into pathogenic forms.

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    Bacterial Transformation

    The process where bacteria take up foreign DNA and change characteristics.

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    Prokaryotes

    Unicellular organisms with no true nucleus, like bacteria.

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    Yeast as a Model Organism

    Objectives include sequencing the human genome and addressing ethical issues.

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    Radioisotope Labeling

    Using radioactive isotopes to trace DNA and proteins during experiments.

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    Transfer of 32P

    The process of radioactive phage DNA entering the cytoplasm of E. coli.

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    Study Notes

    Molecular Biology - Lecture 1

    • Molecular biology studies the composition, structure, and interactions of cellular molecules like nucleic acids and proteins. These molecules carry out biological processes for cell function and maintenance.

    Historical Perspective

    • DNA, despite its current prominence, was only discovered relatively recently in the history of biology.
    • Gregor Mendel's work on heredity predated the discovery of DNA by a century.
    • Early 20th century scientists rediscovered Mendel's principles and realized genes play a crucial role in inheritance.
    • The discovery of DNA's structure initiated a new era in biology, leading to the understanding of the genetic code.
    • Advances in DNA sequencing, genetic engineering, and gene cloning further elucidated the role of DNA.
    • The complete sequencing of the human genome (2001) marked a significant milestone.
    • Future research will focus on practical applications of these discoveries in industry, medicine, food, and agriculture. The practical use of such discoveries began early with uses in medicine, food and agriculture.

    Early Experiments

    • Mendel's Pea Plants: Mendel's experiments demonstrated the principles of inheritance through pea plants, looking at patterns of inheritance of traits like flower color. He noticed different combinations of traits passed down from parents, leading to the Law of Segregation which describes that individuals possess two alleles for a trait and pass a single allele to their offspring.

    • Frederick Griffith's Bacterial Transformation: In 1928, Griffith's experiments demonstrated a "transforming principle" .Transferring a genetic substance from one bacterial strain (pathogenic) to another (non-pathogenic) via a process called transformation. Griffith found that live non-pathogenic bacteria transformed into pathogenic bacteria when mixed with killed pathogenic bacteria.

    • Hershey-Chase Experiments: Hershey and Chase (1952) used bacteriophages to show that DNA, not protein, is the genetic material that transfers during viral infection. They tracked radioactive markers of DNA and protein to confirm which entered a bacteria cell, therefore confirming their theory.

    Other Key Concepts

    • The Transforming Principle: The concept relates to the transfer of genetic information/material between cells to alter traits.

    • Genes: The fundamental unit of heredity. Segments of DNA carrying information for proteins or RNA.

    • Alleles: Different forms of a gene that occupy the same locus on a chromosome and determine the same trait. Alleles may be identical or different, resulting in homozygous or heterozygous genotypes.

    • Genotype: The genetic makeup of an individual, referring to the combination of alleles.

    • Phenotype: The observable traits or characteristics of an individual.

    • Genome: All the genetic material in a cell, including chromosomes (DNA) and other non-chromosomal DNA like plasmids or mitochondrial DNA.

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