Molecular Biology: Fidelity of DNA Replication
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Questions and Answers

What is the result of semi-conservative replication in terms of DNA strands?

  • Two DNA molecules, each with one parental strand and one daughter strand (correct)
  • Two DNA molecules, one with solely parental strands and one with solely daughter strands
  • One DNA molecule with two parental strands and one with two daughter strands
  • One DNA molecule with alternating segments of parental and daughter DNA

Which model of replication suggests that the two original DNA strands re-basepair with each other after replication?

  • Bidirectional replication
  • Dispersive replication
  • Conservative replication (correct)
  • Semi-conservative replication

What is the characteristic of dispersive replication?

  • Replication occurs in a semi-discontinuous manner
  • Each DNA molecule has one parental and one daughter strand
  • DNA molecules have alternating segments of parental and daughter DNA (correct)
  • New DNA strands are synthesized in one direction

Which experiment supported Watson and Crick's hypothesis of semi-conservative replication?

<p>Meselson-Stahl experiment (D)</p> Signup and view all the answers

What is the key feature of semi-conservative replication in terms of DNA synthesis?

<p>Each of the two parental DNA strands acts as a template for new DNA synthesis (B)</p> Signup and view all the answers

What is the result of the Meselson-Stahl experiment in terms of DNA replication?

<p>Semi-conservative replication is the correct model (B)</p> Signup and view all the answers

What is the site where the nucleotide addition occurs during DNA synthesis?

<p>Insertion site (C)</p> Signup and view all the answers

What is the frequency of errors in DNA replication in E. coli?

<p>One error per 1000 to 10,000 replications (A)</p> Signup and view all the answers

According to the Meselson and Stahl experiment, what is the composition of each new double-stranded DNA helix?

<p>One strand from the original helix and one newly synthesized (C)</p> Signup and view all the answers

What is the primary mechanism used by enzymes to ensure high fidelity during DNA replication?

<p>Base selection and proofreading (B)</p> Signup and view all the answers

What technique did Meselson and Stahl use to distinguish between parent and newly copied DNA?

<p>Isotopic labeling of nitrogen (D)</p> Signup and view all the answers

What is the term for the process of DNA replication demonstrated by the Meselson and Stahl experiment?

<p>Semi-conservative replication (D)</p> Signup and view all the answers

What is the function of the 3′→5′ exonuclease activity in DNA polymerase?

<p>To remove mismatched nucleotides during proofreading (C)</p> Signup and view all the answers

Which DNA polymerase is the principal replication polymerase?

<p>DNA polymerase III (A)</p> Signup and view all the answers

What is the significance of the Meselson and Stahl experiment in the context of DNA replication?

<p>It proved that DNA replication occurs through a semi-conservative mechanism (C)</p> Signup and view all the answers

What is the term for atoms of the same element that have the same number of protons but a different number of neutrons in their nuclei?

<p>Isotopes (B)</p> Signup and view all the answers

What is the purpose of proofreading during DNA replication?

<p>To correct mismatched nucleotides during DNA synthesis (A)</p> Signup and view all the answers

What was the model system used by Meselson and Stahl in their famous experiment on DNA replication?

<p>E. coli bacteria (D)</p> Signup and view all the answers

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