Molecular Biology: Fidelity of DNA Replication

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What is the result of semi-conservative replication in terms of DNA strands?

Two DNA molecules, each with one parental strand and one daughter strand (correct)

Two DNA molecules, one with solely parental strands and one with solely daughter strands

One DNA molecule with two parental strands and one with two daughter strands

One DNA molecule with alternating segments of parental and daughter DNA

Which model of replication suggests that the two original DNA strands re-basepair with each other after replication?

Bidirectional replication

Dispersive replication

Conservative replication (correct)

Semi-conservative replication

What is the characteristic of dispersive replication?

Replication occurs in a semi-discontinuous manner

Each DNA molecule has one parental and one daughter strand

DNA molecules have alternating segments of parental and daughter DNA (correct)

New DNA strands are synthesized in one direction

Which experiment supported Watson and Crick's hypothesis of semi-conservative replication?

Meselson-Stahl experiment Signup and view all the answers

What is the key feature of semi-conservative replication in terms of DNA synthesis?

Each of the two parental DNA strands acts as a template for new DNA synthesis Signup and view all the answers

What is the result of the Meselson-Stahl experiment in terms of DNA replication?

Semi-conservative replication is the correct model Signup and view all the answers

What is the site where the nucleotide addition occurs during DNA synthesis?

Insertion site Signup and view all the answers

What is the frequency of errors in DNA replication in E. coli?

One error per 1000 to 10,000 replications Signup and view all the answers

According to the Meselson and Stahl experiment, what is the composition of each new double-stranded DNA helix?

One strand from the original helix and one newly synthesized Signup and view all the answers

What is the primary mechanism used by enzymes to ensure high fidelity during DNA replication?

Base selection and proofreading Signup and view all the answers

What technique did Meselson and Stahl use to distinguish between parent and newly copied DNA?

Isotopic labeling of nitrogen Signup and view all the answers

What is the term for the process of DNA replication demonstrated by the Meselson and Stahl experiment?

Semi-conservative replication Signup and view all the answers

What is the function of the 3′→5′ exonuclease activity in DNA polymerase?

To remove mismatched nucleotides during proofreading Signup and view all the answers

Which DNA polymerase is the principal replication polymerase?

DNA polymerase III Signup and view all the answers

What is the significance of the Meselson and Stahl experiment in the context of DNA replication?

It proved that DNA replication occurs through a semi-conservative mechanism Signup and view all the answers

What is the term for atoms of the same element that have the same number of protons but a different number of neutrons in their nuclei?

Isotopes Signup and view all the answers

What is the purpose of proofreading during DNA replication?

To correct mismatched nucleotides during DNA synthesis Signup and view all the answers

What was the model system used by Meselson and Stahl in their famous experiment on DNA replication?

E. coli bacteria Signup and view all the answers