Molecular Biology: DNA Replication Overview
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Questions and Answers

What is the role of DNA ligase in the replication process?

  • It elongates the DNA chain by adding nucleotides.
  • It corrects errors during DNA replication.
  • It introduces nicks to facilitate unwinding of DNA.
  • It seals the nicks between Okazaki fragments on the lagging strand. (correct)

Which of the following correctly describes a characteristic shared by DNA polymerases?

  • They can carry out mitosis.
  • They are ATP-dependent enzymes.
  • They exclusively bind to the leading strand.
  • They have a proofreading function and can elongate chains. (correct)

What is a function of DNA topoisomerases during DNA replication?

  • They synthesize Okazaki fragments.
  • They seal nicks using ATP.
  • They unwind supercoiled DNA and introduce nicks. (correct)
  • They correct proofreading errors.

During which phase of the cell cycle does DNA replication occur?

<p>S phase (B)</p> Signup and view all the answers

What initiates the transition between different phases of the cell cycle?

<p>Activation of cyclin proteins (C)</p> Signup and view all the answers

Which protein is primarily responsible for unwinding the DNA during replication?

<p>DNA helicase (D)</p> Signup and view all the answers

What is the role of the RNA primer in DNA synthesis?

<p>It serves as an acceptor for the first nucleotide from DNA polymerase. (D)</p> Signup and view all the answers

In which phase of the cell cycle does DNA replication occur?

<p>S phase (A)</p> Signup and view all the answers

What structural formation is described as a 'V' shape where active synthesis occurs?

<p>Replication fork (D)</p> Signup and view all the answers

What is the primary function of Single Stranded DNA-binding proteins (SSBs) in DNA replication?

<p>To stabilize unwound ssDNA and prevent reannealing (C)</p> Signup and view all the answers

Which component of the replication fork is NOT involved in the actual synthesis of the DNA strand?

<p>DNA helicase (A)</p> Signup and view all the answers

What defines the origin of replication (ori) in bacterial DNA?

<p>It is a specific sequence of bases where replication begins. (D)</p> Signup and view all the answers

Which complex is formed by helicase and primase that aids in the initiation of DNA synthesis?

<p>Primosome (D)</p> Signup and view all the answers

What is the primary role of DNA polymerase I during DNA replication?

<p>Excises RNA primers and replaces them with DNA nucleotides (D)</p> Signup and view all the answers

In which direction does DNA polymerase III synthesize new DNA strands?

<p>5' to 3' (A)</p> Signup and view all the answers

Which strand is synthesized continuously and requires only one RNA primer?

<p>Leading strand (C)</p> Signup and view all the answers

What are Okazaki fragments associated with?

<p>Lagging strand synthesis (D)</p> Signup and view all the answers

What distinguishes the leading strand from the lagging strand during DNA replication?

<p>Leading strand is synthesized towards the replication fork (D)</p> Signup and view all the answers

What role do single-strand binding proteins (SSBPs) play during DNA replication?

<p>Preventing premature reannealing of dsDNA (D)</p> Signup and view all the answers

What is the main purpose of topoisomerases in the replication process?

<p>Relieve torsional strain from unwinding DNA (A)</p> Signup and view all the answers

How are newly replicated DNA strands assembled back into chromatin structure?

<p>By reconstitution into nucleosomes (B)</p> Signup and view all the answers

Flashcards

Replication

The process of creating a new DNA molecule from a pre-existing one.

Origin of Replication

A specific sequence on a DNA molecule where replication begins.

Replication Fork

The Y-shaped structure that forms during DNA replication where the DNA strands separate.

DNA Helicase

An enzyme that unwinds the DNA double helix during replication.

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Primase

An enzyme that synthesizes short RNA sequences called RNA primers.

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RNA Primer

A short RNA segment needed for DNA polymerase to start adding DNA nucleotides.

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DNA Polymerase

Enzyme that adds DNA nucleotides to the RNA primer and extends the new DNA strand.

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Bidirectional Replication

DNA replication that proceeds in both directions from an origin of replication.

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DNA Ligase Function

DNA ligase seals nicks in the lagging strand during DNA replication, joining Okazaki fragments.

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DNA Polymerase Function

DNA polymerase elongates new DNA strands and corrects errors during replication.

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DNA Replication Phase

DNA replication occurs during the S phase of the cell cycle.

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Cell Cycle Phases

The cell cycle consists of G1, S, G2, and M (mitotic) phases, with specific events and checkpoints in each phase.

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Swivels in DNA

Swivels interspersed in the DNA molecule during unwinding, introduced by enzymes which create nicks, allowing for unwinding.

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DNA Replication Polarities

DNA synthesis always proceeds in the 5' to 3' direction, but the two template strands are antiparallel, leading to discontinuous synthesis on one strand.

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Leading Strand Synthesis

Synthesized continuously towards the replication fork, requiring only one RNA primer

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Lagging Strand Synthesis

Synthesized discontinuously away from the replication fork, in short fragments (Okazaki fragments) each primed by RNA.

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Okazaki Fragments

Short DNA fragments synthesized on the lagging strand during DNA replication

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RNA Primer Removal

DNA polymerase I replaces RNA primers with DNA nucleotides; DNA ligase seals the gaps.

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DNA Polymerase III

Enzyme responsible for synthesizing the majority of DNA during replication.

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Semi-discontinuous Replication

DNA replication where one strand is synthesized continuously (leading) and the other discontinuously (lagging).

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Role of DNA Polymerase I

Removes RNA primers and fills the gaps with DNA nucleotides during DNA replication.

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