Molecular Biology Chapter Quiz

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Questions and Answers

What is the main function of alternative splicing?

  • To increase the number of genes in the genome
  • To create different mRNA transcripts from a single primary RNA transcript (correct)
  • To create different DNA sequences from a single gene
  • To change the frequency of mutagenesis

What is the role of snRNPs in alternative splicing?

  • They are involved in the processing and splicing of primary RNA transcripts. (correct)
  • They code for the amino acid sequence of proteins.
  • They are responsible for the transportation of mRNA from the nucleus to the cytoplasm.
  • They act as catalysts for DNA replication.

Which of the following is NOT a reason why a three-base codon is necessary to code for all 20 amino acids?

  • A three-base codon provides sufficient combinations to code for all 20 amino acids and more.
  • A two-base codon could only code for 16 amino acids.
  • A three-base codon is necessary to regulate the frequency of mutagenesis. (correct)
  • A one-base codon could only code for four amino acids.

How does alternative splicing contribute to cancer cell development?

<p>It can produce proteins that promote uncontrolled cell division. (D)</p> Signup and view all the answers

Which of the following is a characteristic of the genetic code?

<p>It is a universal language of genetics. (A)</p> Signup and view all the answers

What is the primary function of transfer RNA (tRNA) during translation?

<p>To transport amino acids to the ribosome for protein synthesis. (B)</p> Signup and view all the answers

The "wobble hypothesis" refers to:

<p>The ability of a single tRNA to recognize multiple codons. (A)</p> Signup and view all the answers

Which of the following statements accurately describes the relationship between translation and transcription in prokaryotes?

<p>Transcription and translation are coupled and occur simultaneously. (B)</p> Signup and view all the answers

What is the key factor determining the final three-dimensional structure of a protein?

<p>The sequence of amino acids in the polypeptide chain. (B)</p> Signup and view all the answers

Why is redundancy in the genetic code important?

<p>It protects against mutations that could alter the amino acid sequence. (C)</p> Signup and view all the answers

What is the main function of telomerase?

<p>To extend the ends of chromosomes (D)</p> Signup and view all the answers

Why is telomerase sometimes referred to as the 'fountain of youth'?

<p>It can extend lifespan by preventing the shortening of telomeres. (C)</p> Signup and view all the answers

What is the primary reason for the use of AT-rich sequences in promoter regions?

<p>AT-rich sequences are easily unwound, allowing RNA polymerase to bind. (D)</p> Signup and view all the answers

What is the main difference between DNA and RNA?

<p>All of the above (D)</p> Signup and view all the answers

What is the main purpose of RT-PCR?

<p>To amplify RNA sequences from a small sample. (C)</p> Signup and view all the answers

What is the main role of RNA polymerase in transcription?

<p>All of the above (D)</p> Signup and view all the answers

How does the human genome project demonstrate the application of DNA replication knowledge?

<p>It used DNA sequencing techniques to map the entire human genome. (B)</p> Signup and view all the answers

During translation, what is the primary role of tRNA?

<p>To carry amino acids to the ribosome for protein synthesis. (A)</p> Signup and view all the answers

What is the significance of the 'wobble' phenomenon in translation?

<p>It allows for a single tRNA to recognize multiple codons. (C)</p> Signup and view all the answers

What is the role of the 'A' site in a ribosome?

<p>It is where the tRNA carrying the next amino acid to be added to the polypeptide chain binds. (A)</p> Signup and view all the answers

Which of the following is NOT a factor involved in protein folding?

<p>The concentration of RNA in the cytoplasm. (A)</p> Signup and view all the answers

What is the primary reason why a change in a DNA sequence does not immediately lead to a mutation?

<p>The change may be detected and corrected by proofreading mechanisms during DNA replication. (B)</p> Signup and view all the answers

What is the difference between a primary structure and a secondary protein structure?

<p>The primary structure is the sequence of amino acids, while the secondary structure refers to local interactions between amino acids on the backbone, resulting in alpha helices and beta sheets. (B)</p> Signup and view all the answers

Which DNA repair mechanism involves the removal of a mutated DNA segment and its replacement with a correct sequence?

<p>Excision repair (C)</p> Signup and view all the answers

Which of the following bonds contributes significantly to the tertiary structure of a protein?

<p>All of the above. (D)</p> Signup and view all the answers

Which type of protein structure is characterized by interactions between multiple polypeptide chains?

<p>Quaternary. (C)</p> Signup and view all the answers

What distinguishes chromosomal insertions/deletions from frame shift mutations?

<p>Chromosomal insertions/deletions involve entire segments of chromosomes, while frame shift mutations involve insertions or deletions of individual nucleotides. (A)</p> Signup and view all the answers

Which chromosomal mutation involves a segment of the chromosome breaking off and attaching to a different chromosome?

<p>Translocation (D)</p> Signup and view all the answers

What is the significance of the CCA moiety at the 3' end of a tRNA molecule?

<p>It is the site of amino acid attachment. (B)</p> Signup and view all the answers

What is the significance of the Philadelphia chromosome?

<p>It is a translocation mutation associated with chronic myelogenous leukemia. (C)</p> Signup and view all the answers

Which of the following is an example of a secondary protein structure?

<p>Both A and B. (A)</p> Signup and view all the answers

What is the primary characteristic of transposable elements (TEs)?

<p>They are able to move from one location to another within the genome. (B)</p> Signup and view all the answers

Why is it important to ensure that the tRNA molecule in the host cell has the appropriate anticodon for a particular protein being synthesized?

<p>To ensure the correct amino acid is added to the growing polypeptide chain. (D)</p> Signup and view all the answers

How does proofreading contribute to DNA stability?

<p>Proofreading ensures the accurate replication of DNA by detecting and correcting errors during DNA synthesis. (C)</p> Signup and view all the answers

Which of the following is NOT a mechanism involved in DNA repair?

<p>Transcription repair (A)</p> Signup and view all the answers

In the context of bacterial gene regulation, what does 'repressible' mean?

<p>The operon is always active unless a repressor protein binds to the operator region, blocking transcription. (B)</p> Signup and view all the answers

Which of the following is NOT a characteristic of an operon?

<p>They are found primarily in eukaryotic organisms. (A)</p> Signup and view all the answers

How does the tryptophan (Trp) operon function?

<p>The presence of tryptophan represses the operon, preventing further tryptophan production. (C)</p> Signup and view all the answers

The lac operon is an example of inducible gene regulation. What does this mean?

<p>The operon is active when the inducer is present and inactive when the inducer is absent. (C)</p> Signup and view all the answers

Which of the following is NOT a gene encoded by the lac operon?

<p>Lactase (B)</p> Signup and view all the answers

What is the primary advantage of bacteria having operons?

<p>All of the above. (D)</p> Signup and view all the answers

What is the primary function of the 'permease' protein encoded by the lac operon?

<p>It transports lactose into the bacterial cell. (A)</p> Signup and view all the answers

Why is lactose metabolism considered a catabolic pathway?

<p>Lactose metabolism involves the breakdown of complex molecules. (D)</p> Signup and view all the answers

Flashcards

Alternative Splicing

The process where a single primary RNA transcript can be processed to produce different mature mRNA transcripts, leading to various proteins.

snRNPs

Small nuclear ribonucleoproteins involved in the splicing of pre-mRNA to form mature mRNA.

Codon

A sequence of three nucleotides that together form a unit of genetic code corresponding to a specific amino acid.

Genetic Code

The universal language of genetics that dictates how sequences of nitrogenous bases relate to proteins in all living organisms.

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Start Codon

The specific codon AUG, which signifies the beginning of protein synthesis.

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Telomerase

An enzyme that synthesizes DNA using RNA as a template to repair telomeres.

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Ribonucleoprotein

A complex made of RNA and protein, crucial for many cellular processes.

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Complementary RNA sequence

An RNA sequence that pairs with DNA sequences, like TTAGGG.

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Exogenous telomerase

Telomerase from outside the organism, often tested for therapeutic effects.

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PCR (Polymerase Chain Reaction)

A technique to amplify specific DNA sequences in vitro.

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RT-PCR

A modification of PCR that allows for the amplification of RNA templates.

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Transcription in eukaryotes

The process of synthesizing RNA from a DNA template within the nucleus.

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RNA modifications

Changes made to RNA after transcription before it exits to the cytoplasm.

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Wobble hypothesis

The idea that the third base of a codon can vary while the first two bases are the same, allowing redundancy in amino acid coding.

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Translation

The process of converting mRNA into proteins, occurring in the cytoplasm in eukaryotes with the help of ribosomes and tRNA.

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Essential amino acids

Amino acids that cannot be synthesized by the body and must be obtained from the diet; there are 9 essential amino acids.

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Non-essential amino acids

Amino acids that can be synthesized by the body; there are 11 non-essential amino acids.

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tRNA (transfer RNA)

RNA species that carries amino acids to the ribosome during protein synthesis and has anticodons that pair with mRNA codons.

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Operons

Genes controlled by a single regulatory sequence that can produce polycistronic mRNA.

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Polycistronic mRNA

A long strand of mRNA that contains codes for multiple genes.

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Anabolism

The metabolic process of building up molecules, such as proteins.

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Trp Operon

A repressible operon involved in the synthesis of tryptophan, an amino acid.

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Catabolism

The metabolic process of breaking down molecules to release energy.

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Lac Operon

An inducible operon that facilitates the breakdown of lactose when present.

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Repressor protein

A protein that binds to DNA and inhibits transcription when active.

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tRNA

Transfer RNA that carries amino acids to ribosomes for protein synthesis.

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Anticodon

A three-base sequence on tRNA that matches the mRNA codon.

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Ribosomal RNA (rRNA)

RNA component of ribosomes, essential for protein synthesis.

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EPA Sites

Three binding sites in ribosomes: E (exit), P (peptidyl), A (arrival).

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Amino Acyl Transferase

Enzymes that charge tRNA with the correct amino acid.

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Translation Phases

The three stages of translation: initiation, elongation, termination.

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Primary Structure

The linear sequence of amino acids in a protein.

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Secondary Structure

Localized folding of the amino acid chain, like alpha helices and beta sheets.

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Tertiary Structure

The 3D shape of a protein, determined by long-range interactions between amino acids.

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Quaternary Structure

The arrangement of multiple polypeptide chains in a protein.

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DNA Proofreading

A mechanism where DNA checks and corrects errors during synthesis.

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Mismatch Repair

Enzymes that fix incorrectly paired nucleotides after DNA replication.

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Excision Repair

A repair process that removes and replaces damaged DNA segments.

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Chromosomal Deletion

A type of chromosomal mutation where part of a chromosome is missing.

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Chromosomal Insertion

A chromosomal mutation where extra DNA is added to a chromosome.

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Chromosomal Inversion

A mutation where a segment of a chromosome breaks off and reattaches backwards.

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Transposable Elements

DNA sequences that can move and insert themselves into different locations in the genome.

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Frame Shift Mutation

A mutation that alters the reading frame of a gene due to insertions or deletions.

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Study Notes

Biotechnology in the News Notes

  • Highlighted text signifies important foundational biological concepts repeatedly discussed throughout the course.
  • Exam review should include questions asked in class.
  • Relevant applications, products, and examples of biotechnology are highlighted in green.
  • Tools are highlighted in blue.
  • Lecture notes contain footnotes with helpful review material.

Table of Contents

  • Lecture 1: Introduction to Biotechnology (Jan 7, 9, 2025) including class expectations, listed on slide 2, and important communication guidelines concerning TAs and Dr. Bhasin.
  • Lecture 2: Cell Structure (Jan 9, 14, 2025), with a worksheet.
  • Lecture 3: Molecules of Life (Jan 14, 16, 2025).
  • Lecture 4: Transcription and Translation (Jan 16, 21, 2025).
  • Lecture 5: Control of Gene Expression (Jan 23, 28, 2025).
  • Lecture 6: Mutations: Causes and Consequences (Jan 28, 30, 2025).
  • Lecture 7: Introduction to Recombinant DNA (date not provided).
  • Lecture 8: Forensics (date not provided).

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