BIO-31: Ch.8 part 1
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Questions and Answers

What is the role of mRNA in translation?

  • It forms the structure of the ribosome.
  • It transports amino acids to the ribosome.
  • It catalyzes the formation of peptide bonds.
  • It serves as the template for protein assembly. (correct)

What is a codon?

  • A group of three mRNA nucleotides that code for a particular amino acid. (correct)
  • A type of ribosome.
  • A protein that helps in translation.
  • A single nucleotide in mRNA.

Which of these codons serves as the start codon for translation?

  • UAG
  • AUG (correct)
  • UGA
  • UAA

What type of bond is formed between amino acids during translation?

<p>Peptide bond (B)</p> Signup and view all the answers

What is the study of genes, how they carry information, how information is expressed, and how genes are replicated?

<p>Genetics (A)</p> Signup and view all the answers

Which structure contains DNA that physically carries hereditary information?

<p>Chromosomes (B)</p> Signup and view all the answers

What are segments of DNA that encode functional products called?

<p>Genes (C)</p> Signup and view all the answers

What determines how a nucleotide sequence is converted to an amino acid sequence of a protein?

<p>The genetic code (D)</p> Signup and view all the answers

What is the genetic makeup of an organism called?

<p>Genotype (A)</p> Signup and view all the answers

What term describes the expression of genes?

<p>Phenotype (A)</p> Signup and view all the answers

What type of chromosome do bacteria usually have?

<p>Single circular chromosome (C)</p> Signup and view all the answers

What is the flow of genetic information from one generation to the next called?

<p>Vertical gene transfer (A)</p> Signup and view all the answers

What type of bond holds the two DNA strands together?

<p>Hydrogen bond (B)</p> Signup and view all the answers

Which of the following is NOT a nucleotide found in DNA?

<p>Uracil (A)</p> Signup and view all the answers

What enzyme separates the two strands of DNA during replication?

<p>Helicase (B)</p> Signup and view all the answers

In what direction does DNA polymerase add nucleotides to the growing DNA strand?

<p>5' → 3' (D)</p> Signup and view all the answers

Which of the following describes the arrangement of the two DNA strands?

<p>Antiparallel (A)</p> Signup and view all the answers

What is the function of DNA ligase?

<p>To join Okazaki fragments (B)</p> Signup and view all the answers

Which enzyme relaxes the DNA strands during replication?

<p>Topoisomerase/Gyrase (B)</p> Signup and view all the answers

What initiates DNA synthesis?

<p>RNA primer (A)</p> Signup and view all the answers

What are the short, newly synthesized DNA fragments formed on the lagging template strand during DNA replication, called?

<p>Okazaki fragments (A)</p> Signup and view all the answers

What is the overall structure of DNA?

<p>Double helix (C)</p> Signup and view all the answers

Which enzyme relaxes supercoiling ahead of the replication fork?

<p>DNA Gyrase (A)</p> Signup and view all the answers

Which of the following is a characteristic of bacterial DNA replication?

<p>Bidirectional (B)</p> Signup and view all the answers

What is the primary function of DNA ligase?

<p>Joining DNA strands (B)</p> Signup and view all the answers

What is the role of DNA polymerase in DNA replication?

<p>Proofreading DNA (B)</p> Signup and view all the answers

Which of the following nitrogenous bases is found in RNA but not in DNA?

<p>Uracil (B)</p> Signup and view all the answers

Which enzyme synthesizes DNA?

<p>DNA Polymerase (B)</p> Signup and view all the answers

What is the function of ribosomal RNA (rRNA)?

<p>Forms an integral part of ribosomes (D)</p> Signup and view all the answers

What is the function of exonucleases?

<p>To cut DNA from an exposed end (B)</p> Signup and view all the answers

Which type of RNA carries coded information from DNA to ribosomes?

<p>mRNA (B)</p> Signup and view all the answers

Which enzyme unwinds double-stranded DNA?

<p>Helicase (D)</p> Signup and view all the answers

Where does transcription begin in prokaryotes?

<p>Promoter (C)</p> Signup and view all the answers

What is the role of primase in DNA replication?

<p>Making RNA primers (A)</p> Signup and view all the answers

In what direction does DNA polymerase synthesize DNA?

<p>5' to 3' (B)</p> Signup and view all the answers

In what direction does transcription proceed?

<p>5' → 3' (D)</p> Signup and view all the answers

What enzyme synthesizes mRNA during transcription?

<p>RNA polymerase (B)</p> Signup and view all the answers

What is the primary function of photolyase?

<p>Repairing UV-induced pyrimidine dimers (B)</p> Signup and view all the answers

In eukaryotes, where does transcription occur?

<p>Nucleus (B)</p> Signup and view all the answers

Which of the following provides the energy for DNA replication?

<p>Hydrolysis of phosphate groups on Nucleotides (A)</p> Signup and view all the answers

What are introns?

<p>Regions of DNA that do not code for proteins (C)</p> Signup and view all the answers

What is the error rate of DNA replication?

<p>1 out of $10^9$ or $10^{10}$ bases (D)</p> Signup and view all the answers

Flashcards

Genetics

The study of genes, including how they carry information, how that information is expressed, and how genes are replicated.

Chromosomes

Structures containing DNA that physically carry hereditary information; they contain genes.

Genes

Segments of DNA that encode functional products, usually proteins.

Genetic Code

A set of rules that determines how a nucleotide sequence is converted to an amino acid sequence of a protein.

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Genotype

The genetic makeup (genes) of an organism.

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Phenotype

The expression of the genes.

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Genomics

The molecular study of genomes.

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Vertical Gene Transfer

Flow of genetic information from one generation to the next.

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Translation

The process where mRNA is decoded to produce a protein.

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Codons

Groups of three mRNA nucleotides that specify a particular amino acid.

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Nonsense Codons

Codons that signal the end of translation; do not code for amino acids.

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Anticodon

tRNA sequence that recognizes mRNA codons and carries the corresponding amino acid.

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Start Codon

The start codon (AUG) initiates translation and codes for methionine.

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What is DNA?

A double helix polymer of nucleotides containing adenine, thymine, cytosine, and guanine.

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What is the DNA backbone?

Deoxyribose-phosphate.

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How are DNA strands held together?

Hydrogen bonds between adenine-thymine (A-T) and cytosine-guanine (C-G).

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What does antiparallel mean in DNA?

The strands run in opposite directions.

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What forms the genetic instructions?

The order of nitrogen-containing bases (A, T, C, G).

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What is topoisomerase?

An enzyme that relaxes DNA strands during replication.

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What is Helicase?

An enzyme that separates DNA strands at the replication fork.

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What is a replication fork?

Region where DNA strands are being separated.

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What does DNA polymerase do?

Adds nucleotides to the growing DNA strand only at the 3' end.

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What are Okazaki fragments?

Short DNA fragments synthesized discontinuously on the lagging strand.

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DNA Gyrase

Relaxes supercoiling ahead of the replication fork.

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DNA Ligase

Joins DNA strands together, especially Okazaki fragments.

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DNA Polymerase

Synthesizes new DNA strands and proofreads/repairs DNA.

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Endonucleases

Cuts DNA within a strand to facilitate repair/insertions.

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Exonucleases

Cuts DNA from the exposed ends to facilitate repair.

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Helicase

Unwinds double-stranded DNA at the replication fork.

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Primase

An RNA polymerase that creates RNA primers from a DNA template.

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RNA Polymerase

Copies RNA from a DNA template.

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Topoisomerase

Relaxes supercoiling ahead of the replication fork and separates DNA circles.

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Okazaki Fragments

Short fragments synthesized discontinuously on the lagging strand.

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Bidirectional Replication

DNA replication where copying proceeds in both directions from an origin point.

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Ribonucleic Acid (RNA)

A single-stranded nucleic acid containing ribose sugar and uracil, involved in protein synthesis.

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Ribosomal RNA (rRNA)

RNA that forms part of ribosomes, the protein synthesis machinery.

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Transfer RNA (tRNA)

RNA that carries amino acids to the ribosome during translation for protein synthesis.

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Messenger RNA (mRNA)

RNA that carries the genetic code from DNA to the ribosome for protein synthesis.

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Transcription

The process of synthesizing an RNA strand from a DNA template.

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Promoter Sequence

The DNA sequence where RNA polymerase binds to initiate transcription of a gene.

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Terminator Sequence

The DNA sequence that signals the end of transcription.

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Exons

Regions of eukaryotic DNA that code for proteins.

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