Molecular Biology and Microbiology Quiz

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Questions and Answers

Which pH level can Vibrio cholerae grow in?

  • 8.5 (correct)
  • 5
  • 6
  • 7

Treponema pallidum is typically cultured in which medium?

  • MacConkey agar
  • Blood agar
  • Nutrient agar
  • Serum broth (correct)

A fastidious organism must be grown on what type of medium?

  • Differential medium
  • Selective medium
  • Minimal medium
  • Enriched medium (correct)

Why do bacteria tend to stain more readily with cationic (positively charged) dyes?

<p>Bacteria contain large amounts of acids (D)</p> Signup and view all the answers

What is a distinguishing characteristic of many Pseudomonas isolates that is useful in identification?

<p>Fecal odor (A)</p> Signup and view all the answers

In which type of medium is Treponema pallidum typically cultured?

<p>Serum broth (C)</p> Signup and view all the answers

What pH level can Vibrio cholerae grow in?

<p>8.5 (D)</p> Signup and view all the answers

Fastidious organisms must be grown on what type of medium?

<p>Enriched medium (D)</p> Signup and view all the answers

Bacteria tend to stain more readily with cationic (positively charged) dyes because they:

<p>contain large amounts of acidic substances (C)</p> Signup and view all the answers

A unique characteristic of many isolates of Pseudomonas useful in identification is:

<p>fecal odor (A)</p> Signup and view all the answers

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Study Notes

DNA Polymerase Correction Mechanism

  • DNA Polymerase has the ability to correct base substitutions and prevent misincorporation of nucleotides through its proofreading and editing activity
  • This mechanism ensures high fidelity of DNA replication and repair

Bacterial Growth and Culture

  • Vibrio cholerae can grow in a medium with a pH of 8.5
  • Treponema pallidum requires a specific medium for growth, which is Serum Broth
  • Fastidious organisms need Enriched medium to grow
  • Bacteria have a tendency to stain more readily with cationic (positively charged) dyes due to the presence of large amounts of acidic substances
  • A distinct characteristic of many Pseudomonas isolates that aids in identification is the production of a fecal odor

DNA Polymerase Correction Mechanism

  • DNA Polymerase has the ability to correct base substitutions and prevent misincorporation of nucleotides through its proofreading and editing activity
  • This mechanism ensures high fidelity of DNA replication and repair

Bacterial Growth and Culture

  • Vibrio cholerae can grow in a medium with a pH of 8.5
  • Treponema pallidum requires a specific medium for growth, which is Serum Broth
  • Fastidious organisms need Enriched medium to grow
  • Bacteria have a tendency to stain more readily with cationic (positively charged) dyes due to the presence of large amounts of acidic substances
  • A distinct characteristic of many Pseudomonas isolates that aids in identification is the production of a fecal odor

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