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Molecular Biology and Microbiology Quiz

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10 Questions

Which pH level can Vibrio cholerae grow in?

8.5

Treponema pallidum is typically cultured in which medium?

Serum broth

A fastidious organism must be grown on what type of medium?

Enriched medium

Why do bacteria tend to stain more readily with cationic (positively charged) dyes?

Bacteria contain large amounts of acids

What is a distinguishing characteristic of many Pseudomonas isolates that is useful in identification?

Fecal odor

In which type of medium is Treponema pallidum typically cultured?

Serum broth

What pH level can Vibrio cholerae grow in?

8.5

Fastidious organisms must be grown on what type of medium?

Enriched medium

Bacteria tend to stain more readily with cationic (positively charged) dyes because they:

contain large amounts of acidic substances

A unique characteristic of many isolates of Pseudomonas useful in identification is:

fecal odor

Study Notes

DNA Polymerase Correction Mechanism

  • DNA Polymerase has the ability to correct base substitutions and prevent misincorporation of nucleotides through its proofreading and editing activity
  • This mechanism ensures high fidelity of DNA replication and repair

Bacterial Growth and Culture

  • Vibrio cholerae can grow in a medium with a pH of 8.5
  • Treponema pallidum requires a specific medium for growth, which is Serum Broth
  • Fastidious organisms need Enriched medium to grow
  • Bacteria have a tendency to stain more readily with cationic (positively charged) dyes due to the presence of large amounts of acidic substances
  • A distinct characteristic of many Pseudomonas isolates that aids in identification is the production of a fecal odor

DNA Polymerase Correction Mechanism

  • DNA Polymerase has the ability to correct base substitutions and prevent misincorporation of nucleotides through its proofreading and editing activity
  • This mechanism ensures high fidelity of DNA replication and repair

Bacterial Growth and Culture

  • Vibrio cholerae can grow in a medium with a pH of 8.5
  • Treponema pallidum requires a specific medium for growth, which is Serum Broth
  • Fastidious organisms need Enriched medium to grow
  • Bacteria have a tendency to stain more readily with cationic (positively charged) dyes due to the presence of large amounts of acidic substances
  • A distinct characteristic of many Pseudomonas isolates that aids in identification is the production of a fecal odor

Test your knowledge of DNA polymerase, bacterial growth, and microbiology concepts in this short quiz.

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