10 Questions
What is the recommended technique to avoid cross-contaminants in PCR?
Using laminar flow or special workbench only for PCR
What is the purpose of using nested-PCR?
To increase the specificity of DNA amplification
Which of the following is NOT a method to avoid contamination in PCR?
Storing reagents in large quantities
How many sets of primers are used in nested-PCR?
Two sets
What results from using one pair of primers in the first PCR reaction in nested-PCR?
Target DNA products for the second reaction
What is the negative control in PCR?
A complete mix without template DNA
Why is it recommended to use filter tips in PCR?
To avoid cross-contaminants
What technique is suggested for preparing the PCR master mix?
Preparing it on a laminar flow or special PCR workbench
What is an effective way to store PCR reagents?
In small quantities sufficient for one reaction
What technique is used to verify the success of a PCR reaction?
Agarose gel electrophoresis
Test your knowledge on PCR basics, including reaction components, types of templates, primers, and DNA nucleotides. Learn about the different sources of DNA that can be used for PCR amplification.
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