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Questions and Answers
What is the recommended technique to avoid cross-contaminants in PCR?
What is the recommended technique to avoid cross-contaminants in PCR?
- Storing PCR reagents in large quantities
- Using the same tips for all reagents
- Preparing the reaction mix in a non-specific area
- Using laminar flow or special workbench only for PCR (correct)
What is the purpose of using nested-PCR?
What is the purpose of using nested-PCR?
- To increase the specificity of DNA amplification (correct)
- To introduce contamination in PCR reactions
- To decrease the specificity of DNA amplification
- To amplify DNA fragments in a single reaction
Which of the following is NOT a method to avoid contamination in PCR?
Which of the following is NOT a method to avoid contamination in PCR?
- Using filter tips
- Storing reagents in large quantities (correct)
- Using positive and negative controls
- Using aliquots of reaction
How many sets of primers are used in nested-PCR?
How many sets of primers are used in nested-PCR?
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What results from using one pair of primers in the first PCR reaction in nested-PCR?
What results from using one pair of primers in the first PCR reaction in nested-PCR?
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What is the negative control in PCR?
What is the negative control in PCR?
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Why is it recommended to use filter tips in PCR?
Why is it recommended to use filter tips in PCR?
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What technique is suggested for preparing the PCR master mix?
What technique is suggested for preparing the PCR master mix?
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What is an effective way to store PCR reagents?
What is an effective way to store PCR reagents?
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What technique is used to verify the success of a PCR reaction?
What technique is used to verify the success of a PCR reaction?
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