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Questions and Answers

What is the recommended technique to avoid cross-contaminants in PCR?

Storing PCR reagents in large quantities

Using the same tips for all reagents

Preparing the reaction mix in a non-specific area

Using laminar flow or special workbench only for PCR (correct)

What is the purpose of using nested-PCR?

To increase the specificity of DNA amplification (correct)

To introduce contamination in PCR reactions

To decrease the specificity of DNA amplification

To amplify DNA fragments in a single reaction

Which of the following is NOT a method to avoid contamination in PCR?

Using filter tips

Storing reagents in large quantities (correct)

Using positive and negative controls

Using aliquots of reaction

How many sets of primers are used in nested-PCR?

Two sets (B) Signup and view all the answers

What results from using one pair of primers in the first PCR reaction in nested-PCR?

Target DNA products for the second reaction (C) Signup and view all the answers

What is the negative control in PCR?

A complete mix without template DNA (D) Signup and view all the answers

Why is it recommended to use filter tips in PCR?

To avoid cross-contaminants (C) Signup and view all the answers

What technique is suggested for preparing the PCR master mix?

Preparing it on a laminar flow or special PCR workbench (A) Signup and view all the answers

What is an effective way to store PCR reagents?

In small quantities sufficient for one reaction (A) Signup and view all the answers

What technique is used to verify the success of a PCR reaction?

Agarose gel electrophoresis (C) Signup and view all the answers