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MM6113 Molecular Medicine: Reverse Transcription Quiz

Test your knowledge on reverse transcription, including the process, events, and inhibitors, with a focus on Human Immunodeficiency Virus (HIV) replication and cDNA synthesis.

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Questions and Answers

What is the main purpose of DNA replication?

To generate an identical copy of genetic information for daughter cells

Which bases are involved in the AT/GC rule during DNA replication?

Adenine, Thymine, Guanine, Cytosine

What is the significance of the antiparallel alignment in DNA strands during replication?

It allows for complementary base pairing

Which type of bases form purines and pyrimidines in DNA replication?

<p>Adenine &amp; Guanine; Cytosine, Uracil &amp; Thymine</p> Signup and view all the answers

What is the process of copying genetic materials from the original DNA strands called?

<p>DNA replication</p> Signup and view all the answers

When does DNA replication occur in eukaryotic cell cycle?

<p>S phase</p> Signup and view all the answers

Which protein is responsible for breaking the hydrogen bond between the two DNA strands during DNA replication?

<p>DnaB</p> Signup and view all the answers

What is the name of the site where DNA replication starts in prokaryotes?

<p>OriC site</p> Signup and view all the answers

Which molecule is NOT involved in the initiation step of DNA replication?

<p>RNA polymerase</p> Signup and view all the answers

What is the function of DnaA protein in DNA replication initiation step?

<p>Separating the double helix strand</p> Signup and view all the answers

Which characteristic function of various DNA polymerases is NOT mentioned in the learning outcomes?

<p>Synthesizing RNA primers</p> Signup and view all the answers

In which direction does DNA helicase move along the DNA during replication fork formation?

<p>$5' \to 3'$</p> Signup and view all the answers

How many types of DNA sequences are found within OriC in prokaryotes?

<p>3</p> Signup and view all the answers

Which molecule recognizes the AT-rich region and DnaA box sequences at the origin of replication in prokaryotes?

<p>$DnaA$ protein</p> Signup and view all the answers

'The process of copying genetic materials from the original DNA strands is called?' is an example of a:

<p>Knowledge question.</p> Signup and view all the answers

What is the basis for inheritance according to the provided text?

<p>DNA replication.</p> Signup and view all the answers

Which enzyme is responsible for the synthesis of complementary DNA (cDNA) from an RNA template?

<p>Reverse transcriptase</p> Signup and view all the answers

What is the main function of non-nucleoside reverse transcriptase inhibitors (NNRTIs) in the context of HIV treatment?

<p>Inhibit the activity of reverse transcriptase enzyme</p> Signup and view all the answers

In the context of reverse transcription, what is the significance of integrating viral DNA (cDNA) into host DNA?

<p>To create a provirus for long-term persistence</p> Signup and view all the answers

Which type of virus is HIV classified as?

<p>Retrovirus</p> Signup and view all the answers

What is the role of viral protease in the life cycle of HIV?

<p>Packaging and releasing viral RNA from the host cell</p> Signup and view all the answers

Which enzyme is essential for the reverse transcription of HIV?

<p>Reverse transcriptase</p> Signup and view all the answers

What is the significance of reverse transcription in the replication cycle of HIV?

<p>It enables the virus to integrate into the host genome</p> Signup and view all the answers

Which class of drugs targets reverse transcriptase as a key component of their mechanism of action?

<p>(NRTIs) Nucleoside Reverse Transcriptase Inhibitors</p> Signup and view all the answers

What role does integrase play in the replication cycle of HIV?

<p>Integrating viral DNA into host DNA</p> Signup and view all the answers

What is the primary target of Human Immunodeficiency Virus (HIV) infection?

<p>T Helper cells (CD4+)</p> Signup and view all the answers

What is the role of viral integrase in the replication cycle of HIV?

<p>Inserting viral DNA into the host’s genome</p> Signup and view all the answers

What is the main function of non-nucleoside reverse transcriptase inhibitors (NNRTIs) in the context of HIV treatment?

<p>Preventing the synthesis of viral DNA from an RNA template</p> Signup and view all the answers

What enzyme is essential for the reverse transcription of HIV?

<p>Reverse transcriptase</p> Signup and view all the answers

Which molecule recognizes the AT-rich region and DnaA box sequences at the origin of replication in prokaryotes?

<p>DnaA protein</p> Signup and view all the answers

Which characteristic function of various DNA polymerases is NOT mentioned in the learning outcomes?

<p>Synthesizing the complementary DNA from an RNA template</p> Signup and view all the answers

When does DNA replication occur in eukaryotic cell cycle?

<p>S phase</p> Signup and view all the answers

What is the primary target of Human Immunodeficiency Virus (HIV) infection?

<p>T helper cells (CD4+ cells)</p> Signup and view all the answers

What is the significance of integrating viral DNA (cDNA) into host DNA in the context of reverse transcription?

<p>It allows HIV to avoid detection by the immune system and persist in the host</p> Signup and view all the answers

What is the main purpose of DNA replication?

<p>Ensuring accurate transmission of genetic information to daughter cells</p> Signup and view all the answers

What bases are involved in the AT/GC rule during DNA replication?

<p>Cytosine and thymine</p> Signup and view all the answers

What enzyme is responsible for breaking the hydrogen bond between two DNA strands during DNA replication?

<p>DNA helicase</p> Signup and view all the answers

What type of virus is HIV classified as?

<p>Retrovirus</p> Signup and view all the answers

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