MLBT1080 Enterobacteriaceae MCQs

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Questions and Answers

Which of the following is true about Enterobacteriaceae?

  • They are Gram-positive bacilli
  • They are Gram-negative bacilli (correct)
  • They form spores
  • They do not form spores

What is the common term used to refer to the Family Enterobacteriaceae?

  • Cocci
  • Coliforms
  • Enterics (correct)
  • Bacilli

Which of the following is NOT one of the four major features shared by Enterobacteriaceae?

  • Oxidase-negative
  • Reduce nitrates to nitrites
  • Motile (correct)
  • Ferment glucose

Which of the following Enterobacteriaceae is NOT included in the list of non-motile organisms?

<p>Escherichia (A)</p> Signup and view all the answers

What is the biochemical test used to distinguish Enterobacteriaceae from other Gram-negative bacteria?

<p>Nitrate reduction test (C)</p> Signup and view all the answers

What is the value of colony morphology on BAP or CA in distinguishing Enterobacteriaceae?

<p>It has little value in distinguishing them, except for Klebsiella (C)</p> Signup and view all the answers

Which of the following media are used for the initial evaluation of colonies of Enterobacteriaceae?

<p>MacConkey, HE, and XLD agars (A)</p> Signup and view all the answers

Which of the following is not true about Enterobacteriaceae?

<p>Their colony morphology on BAP or CA is of great value in distinguishing them (D)</p> Signup and view all the answers

What is the basis of classification for Enterics?

<p>Tribe (B)</p> Signup and view all the answers

How are species further grouped within each Tribe?

<p>By biochemical characteristics (A)</p> Signup and view all the answers

What is the purpose of grouping Enterobacteriaceae into Tribes?

<p>To group species with similar characteristics (C)</p> Signup and view all the answers

Which of the following is not a part of the Enterobacteriaceae classification system?

<p>Kingdom (A)</p> Signup and view all the answers

What is the O antigen in enteric bacteria?

<p>Somatic and heat-stable antigen (D)</p> Signup and view all the answers

Which of the following contributes to the pathogenicity of enteric bacteria?

<p>Ability to invade tissues (C)</p> Signup and view all the answers

What is the H antigen in enteric bacteria?

<p>Flagellar antigen (D)</p> Signup and view all the answers

Which of the following is a heat-labile antigen in enteric bacteria?

<p>Both b and c (B)</p> Signup and view all the answers

What is the function of plasmids in enteric bacteria?

<p>To mediate resistance to antibiotics (C)</p> Signup and view all the answers

Which of the following antigens is located in the cell wall of enteric bacteria?

<p>O antigen (C)</p> Signup and view all the answers

What is the meaning of 'fecal coliforms' in reference to Enterics?

<p>Enterics found in the feces of animals and humans (B)</p> Signup and view all the answers

Where can Enterics be found besides the intestinal tract of animals and humans?

<p>In the soil and water (A)</p> Signup and view all the answers

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Study Notes

Overview of Enterobacteriaceae

  • Enterobacteriaceae are classified as Gram-negative bacilli and do not form spores.
  • Commonly referred to as "Enterics," the family includes various pathogenic and non-pathogenic species.

Key Features of Enterobacteriaceae

  • Major characteristics: ferment glucose and reduce nitrates to nitrites.
  • Enterobacteriaceae are predominantly oxidase-negative, with motility varied among species.

Identification and Biochemical Testing

  • Escherichia species are motile, while Klebsiella, Shigella, and Yersinia are non-motile.
  • The nitrate reduction test is vital for distinguishing Enterobacteriaceae from other Gram-negative bacteria.
  • Colony morphology on Blood Agar Plates (BAP) or Chocolate Agar (CA) has limited value, primarily helpful for identifying Klebsiella.

Media for Primary Evaluation

  • Initial colony evaluation typically involves media like MacConkey, Hektoen Enteric (HE), and Xylose Lysine Deoxycholate (XLD) agars.

Classification Structure

  • Enterobacteriaceae are classified into tribes, simplifying their classification based on shared characteristics.
  • Further grouping within each tribe is based on biochemical characteristics, not colony morphology.

Antigenic Components

  • The O antigen is a somatic, heat-stable component found in the cell wall.
  • The H antigen serves as a flagellar antigen, while K antigens are part of the capsule.
  • Heat-labile antigens include both H and K types.

Pathogenicity Factors

  • Pathogenic enteric bacteria often possess the ability to invade tissues, contrasting with non-pathogenic species.
  • Plasmids in enteric bacteria confer antibiotic resistance, critical for their survival.

Environmental Presence

  • Fecal coliforms refer to Enterics found in feces of animals and humans, highlighting their significance in health and sanitation.
  • Beyond intestinal habitats, Enterics can also be located in soil and water, contributing to their environmental resilience and distribution.

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