Podcast
Questions and Answers
What is the main function of NADH in the mitochondrion?
What is the main function of NADH in the mitochondrion?
- To provide electrons for the electron transport chain (correct)
- To directly consume oxygen
- To oxidize acetyl groups in the matrix
- To catalyze the citric acid cycle
Which structural feature of the inner mitochondrial membrane increases its surface area?
Which structural feature of the inner mitochondrial membrane increases its surface area?
- Outer membrane
- Matrix
- Porins
- Cristae (correct)
In what form is energy primarily stored during biological oxidation in mitochondria?
In what form is energy primarily stored during biological oxidation in mitochondria?
- As heat
- As water
- As ATP (correct)
- As NADH
Which of the following statements is true regarding the citric acid cycle?
Which of the following statements is true regarding the citric acid cycle?
What role do cristae play in mitochondrial function?
What role do cristae play in mitochondrial function?
What triggers the energetically favorable reaction of H2 and O2 to form water in mitochondria?
What triggers the energetically favorable reaction of H2 and O2 to form water in mitochondria?
How does the mitochondrial enzyme activity vary?
How does the mitochondrial enzyme activity vary?
What happens during the process of oxidative phosphorylation?
What happens during the process of oxidative phosphorylation?
What drives the flow of H+ through the ATP synthase in mitochondria?
What drives the flow of H+ through the ATP synthase in mitochondria?
How many net ATP are produced from one molecule of glucose during cellular respiration?
How many net ATP are produced from one molecule of glucose during cellular respiration?
In which part of cellular respiration is NADPH produced?
In which part of cellular respiration is NADPH produced?
What role do bacteria play in chemiosmotic mechanisms?
What role do bacteria play in chemiosmotic mechanisms?
What is the main purpose of mitochondria in cell metabolism?
What is the main purpose of mitochondria in cell metabolism?
Which of the following describes strict anaerobes in terms of energy production?
Which of the following describes strict anaerobes in terms of energy production?
Which compound is actively transported from the mitochondrion to the cytosol for NADPH production?
Which compound is actively transported from the mitochondrion to the cytosol for NADPH production?
What type of pathway does ATP synthase create across the inner mitochondrial membrane?
What type of pathway does ATP synthase create across the inner mitochondrial membrane?
What main function do mitochondria serve in eukaryotic cells?
What main function do mitochondria serve in eukaryotic cells?
What is the first electron carrier in the electron-transport chain of mitochondria?
What is the first electron carrier in the electron-transport chain of mitochondria?
Which process describes the flow of protons through ATP synthase?
Which process describes the flow of protons through ATP synthase?
What establishes the electrochemical proton gradient in mitochondria?
What establishes the electrochemical proton gradient in mitochondria?
What is the primary function of NADH in cellular respiration?
What is the primary function of NADH in cellular respiration?
What is chemiosmotic coupling primarily responsible for?
What is chemiosmotic coupling primarily responsible for?
Which statement is true about the endosymbiotic theory?
Which statement is true about the endosymbiotic theory?
Which statement accurately describes the role of NAD+ in mitochondria?
Which statement accurately describes the role of NAD+ in mitochondria?
What role do porins play in the outer membrane of the mitochondrion?
What role do porins play in the outer membrane of the mitochondrion?
Why is the plasma membrane primarily reserved for transport processes in eukaryotic cells?
Why is the plasma membrane primarily reserved for transport processes in eukaryotic cells?
What best describes the function of the electron-transport chain in mitochondria?
What best describes the function of the electron-transport chain in mitochondria?
Which compartment of the mitochondrion contains enzymes for the citric acid cycle?
Which compartment of the mitochondrion contains enzymes for the citric acid cycle?
What is a primary difference between mitochondrial DNA (mtDNA) and chloroplast DNA (cpDNA)?
What is a primary difference between mitochondrial DNA (mtDNA) and chloroplast DNA (cpDNA)?
What role do the electron carriers play in the electron transport chain?
What role do the electron carriers play in the electron transport chain?
Which process explains how lung bacteria became mitochondria in eukaryotic cells?
Which process explains how lung bacteria became mitochondria in eukaryotic cells?
What is the main function of ATP synthase in oxidative phosphorylation?
What is the main function of ATP synthase in oxidative phosphorylation?
How do electrons transfer through the electron transport chain?
How do electrons transfer through the electron transport chain?
What are the two consequences of H+ movement across the inner mitochondrial membrane?
What are the two consequences of H+ movement across the inner mitochondrial membrane?
What are the two separate compartments created by the mitochondrion's membranes?
What are the two separate compartments created by the mitochondrion's membranes?
How does the electrochemical proton gradient influence ATP synthesis?
How does the electrochemical proton gradient influence ATP synthesis?
What is the primary function of the protein complexes in the respiratory chain?
What is the primary function of the protein complexes in the respiratory chain?
Which statement accurately describes the voltage gradient generated by H+ movement?
Which statement accurately describes the voltage gradient generated by H+ movement?
Which of the following accurately represents the relationship between pH and the location of H+ ions?
Which of the following accurately represents the relationship between pH and the location of H+ ions?
What drives the pumping of H+ across the inner membrane during oxidative phosphorylation?
What drives the pumping of H+ across the inner membrane during oxidative phosphorylation?
What is the primary role of the electron transport chain in bacteria?
What is the primary role of the electron transport chain in bacteria?
In some bacteria, how does ATP synthase utilize ATP produced by glycolysis?
In some bacteria, how does ATP synthase utilize ATP produced by glycolysis?
Which of the following statements is true regarding protons and bacterially maintained gradients?
Which of the following statements is true regarding protons and bacterially maintained gradients?
What is NOT a characteristic of mitochondria's outer membrane?
What is NOT a characteristic of mitochondria's outer membrane?
Where is NADH primarily produced within the mitochondria?
Where is NADH primarily produced within the mitochondria?
Which location within the mitochondria contains the respiratory chain?
Which location within the mitochondria contains the respiratory chain?
What is a function of the Na+-H+ antiporter in bacteria?
What is a function of the Na+-H+ antiporter in bacteria?
Which of the following is true about the electrochemical potential for H+ in the intermembrane space?
Which of the following is true about the electrochemical potential for H+ in the intermembrane space?
Flashcards
Mitochondria's Role
Mitochondria's Role
Mitochondria are organelles in eukaryotic cells that produce most of the cell's ATP, using oxygen to convert energy in food molecules into a usable form (ATP).
ATP Synthesis
ATP Synthesis
ATP is synthesized through a process called chemiosmosis, which involves electron transport chains and ATP synthase within the mitochondrial membrane.
Chemiosmotic Coupling
Chemiosmotic Coupling
Chemiosmosis is the process in which energy from a proton gradient across a membrane is used to generate ATP.
Electron Transport Chain (ETC)
Electron Transport Chain (ETC)
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NAD+
NAD+
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ATP synthase
ATP synthase
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Proton Gradient
Proton Gradient
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NADH Function
NADH Function
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Electron Transport Chain (ETC)
Electron Transport Chain (ETC)
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Endosymbiotic Theory
Endosymbiotic Theory
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Mitochondrial DNA (mtDNA)
Mitochondrial DNA (mtDNA)
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Chloroplast DNA (cpDNA)
Chloroplast DNA (cpDNA)
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Mitochondrial Membranes
Mitochondrial Membranes
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Mitochondrial Matrix
Mitochondrial Matrix
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Mitochondrial Membranes
Mitochondrial Membranes
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Citric Acid Cycle Products
Citric Acid Cycle Products
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Respiratory Chain Location
Respiratory Chain Location
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Oxidative Phosphorylation
Oxidative Phosphorylation
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Electron Transport Chain role
Electron Transport Chain role
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ATP Generation
ATP Generation
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Cellular Respiration vs. Combustion
Cellular Respiration vs. Combustion
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Chemiosmotic Process
Chemiosmotic Process
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Role of Oxygen in Respiration
Role of Oxygen in Respiration
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Electron Transport Chain
Electron Transport Chain
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ATP Synthase
ATP Synthase
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Chemiosmosis
Chemiosmosis
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H+ Gradient
H+ Gradient
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pH gradient (ΔpH)
pH gradient (ΔpH)
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Voltage gradient (ΔV)
Voltage gradient (ΔV)
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Oxidative Phosphorylation
Oxidative Phosphorylation
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Respiratory Chain
Respiratory Chain
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ATP synthase
ATP synthase
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Proton Gradient
Proton Gradient
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Cellular Respiration ATP Yield
Cellular Respiration ATP Yield
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Mitochondrial Role
Mitochondrial Role
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Chemiosmosis Energy Source
Chemiosmosis Energy Source
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Bacteria and Chemiosmosis
Bacteria and Chemiosmosis
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Proton Motive Force
Proton Motive Force
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ATP Synthase in Reverse
ATP Synthase in Reverse
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Proton Gradient in Bacteria
Proton Gradient in Bacteria
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Mitochondrial Outer Membrane
Mitochondrial Outer Membrane
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Mitochondrial Size
Mitochondrial Size
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Mitochondria & Cytoskeleton
Mitochondria & Cytoskeleton
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Respiratory Chain Location
Respiratory Chain Location
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NADH Production
NADH Production
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Same H+ potential as cytoplasm
Same H+ potential as cytoplasm
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Porins Location
Porins Location
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Study Notes
Mitochondria
- Mitochondria are organelles found in eukaryotic cells.
- They are responsible for producing most of the ATP (adenosine triphosphate) in the cell.
- Mitochondria have a double membrane.
- The outer membrane is permeable to small molecules.
- The inner membrane has folds called cristae, increasing its surface area.
- The fluid-filled space within the inner membrane is the matrix.
- The intermembrane space is between the inner and outer membranes.
Life Requires Energy
- ATP (adenosine triphosphate) has high-energy bonds.
- Cells use ATP energy for various functions
- Synthesizing cellular molecules
- Cellular movements
- Transport against concentration gradients
- Generating electric potentials across membranes
- Organisms obtain energy from light (photosynthesis) or high-energy compounds (respiration).
Prokaryotes and Eukaryotes
- Prokaryotes produce ATP using their plasma membrane.
- Eukaryotes produce most of their ATP in specialized organelles called mitochondria.
Chemiosmotic Coupling
- The process of producing ATP occurs in two stages.
- Stage 1 involves transferring high-energy electrons through electron carriers in a membrane, releasing energy to pump protons (H+) across the membrane. This builds an electrochemical proton gradient.
- Stage 2 involves H+ flowing back down its electrochemical gradient through a protein called ATP synthase, driving ATP synthesis from ADP and inorganic phosphate.
Oxidative Phosphorylation
- Oxidative phosphorylation is the primary process for ATP generation in mitochondria.
- Electrons from food molecules are transferred to the electron transport chain.
- The electron transport chain releases energy to pump protons, creating an electrochemical gradient.
- ATP synthase uses the energy of the H+ gradient to produce ATP.
Endosymbiotic Theory
- Mitochondria and chloroplasts arose from free-living bacteria.
- These bacteria were engulfed by larger cells, forming a symbiotic relationship.
- Over time, the bacteria lost their ability to function independently.
- Evidence supports the idea that these organelles evolved separately from the host cell.
Mitochondrial Structure
- Mitochondria have an outer and inner membrane.
- The inner membrane is folded into cristae, increasing surface area.
- The matrix is the space enclosed by the inner membrane.
- The intermembrane space lies between the two membranes.
Mitochondrial function
- Mitochondria are responsible for cellular respiration.
- Cellular respiration converts energy from food into ATP for cell use.
- They produce chemical compounds that are used for energy production and other cellular processes.
Mitochondrial DNA (mtDNA)
- mtDNA exists as a double-stranded circular DNA.
- It is present in many copies in a single mitochondrion.
- It is smaller than chloroplast DNA (cpDNA).
Chloroplast DNA (cpDNA)
- cpDNA is double-stranded circular DNA.
- It is smaller than mitochondrial DNA (mtDNA).
- Genes in cpDNA encode products involved in photosynthesis and translation.
ATP Yield
- Glycolysis produces 2 net ATP.
- The citric acid cycle produces 2 ATP.
- Oxidative phosphorylation produces 30 - 32 ATP per glucose molecule.
Other Mitochondrial Roles
- Mitochondria play critical roles in cell metabolism, beyond ATP production.
- They use amino acids as fuel during starvation.
- They supply the cytosol with reducing power (NADPH), used in biosynthesis.
- They also buffer the redox potential in the cytosol.
- Bacteria use chemiosmosis, and pumps H+ out of the cell.
- The H+ gradient is used in various bacterial processes.
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