Millon’s Test for Tyrosine Detection
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Questions and Answers

What is the purpose of Millon's test?

  • To detect the presence of heavy metals in proteins
  • To detect the presence of all amino acids in proteins
  • To detect the presence of tyrosine in proteins (correct)
  • To detect the presence of phenol group in all compounds

    • What is the composition of Millon's reagent?

  • Sodium nitrate and concentrated sulfuric acid
  • Calcium carbonate and acetic acid
  • Mercuric nitrate and mercurous nitrate dissolved in concentrated nitric acid (correct)
  • Potassium chloride and hydrochloric acid

    • What happens to the tyrosine molecule during Millon's test?

  • It reacts with nitric acid to form a yellow-colored precipitate
  • It undergoes nitrification to form a green-colored solution
  • It forms complexes with heavy metals like mercury (correct)
  • It combines with sulfuric acid to produce a white-colored substance

    • Is Millon's test specific for detecting tyrosine in proteins?

    <p>Yes, it is specific only for tyrosine detection</p> Signup and view all the answers

    What is the color of the precipitate or solution formed during Millon's test?

    <p>Red-colored</p> Signup and view all the answers

    Which group of compounds does Millon's test specifically detect?

    <p>Compounds containing the phenol group</p> Signup and view all the answers

    Which color complex is formed when ninhydrin reacts with α-amino groups of proteins and amino acids?

    <p>Violet-pink</p> Signup and view all the answers

    What color do nitrated products of aromatic rings on the side chains of proteins or free amino acids appear as?

    <p>Yellow</p> Signup and view all the answers

    In Millon's test, what color does the solution turn into when made with basic sodium hydroxide?

    <p>Red-brown</p> Signup and view all the answers

    Which protein group specifically answers the Xanthoproteic test?

    <p>Proteins containing a phenol group in the side chain</p> Signup and view all the answers

    What is the color of the complex formed in the Hopkins-Cole test upon reaction with glyoxylic acid and concentrated sulfuric acid?

    <p>Violet</p> Signup and view all the answers

    What kind of product does the presence of nitric acid lead to in proteins or free amino acids?

    <p>Yellow-colored complex</p> Signup and view all the answers

    What color complex is typically formed when α-amino acids react with ninhydrin?

    <p>Blue-purple</p> Signup and view all the answers

    What color do nitrated products of aromatic rings on the side chains of proteins or free amino acids appear as in solution?

    <p>Yellow</p> Signup and view all the answers

    What is the purpose of Millon's test for proteins?

    <p>Specific detection of tyrosine</p> Signup and view all the answers

    What is the color of the complex formed in the Hopkins-Cole test upon reaction with glyoxylic acid and concentrated sulfuric acid?

    <p>Violet</p> Signup and view all the answers

    What happens to the color of the solution in Millon's test when it is made with basic sodium hydroxide?

    <p>Becomes more intense and ranges from yellow to orange to brown</p> Signup and view all the answers

    What kind of product is formed when aromatic rings on the side chains of proteins or free amino acids are treated with concentrated nitric acid?

    <p>Yellow nitrated product</p> Signup and view all the answers

    Study Notes

    Millon's Test

    • Purpose: To detect the presence of tyrosine in proteins
    • Composition of Millon's reagent: Mercury(II) sulfate and sulfuric acid
    • Tyrosine molecule: Forms a red precipitate when treated with Millon's reagent
    • Specificity: Millon's test is not specific for tyrosine and can also detect other phenolic compounds

    Reaction Products

    • Color of the precipitate or solution formed: Red
    • Compounds detected: Phenolic compounds, including tyrosine
    • Color complex formed with ninhydrin: Purple (with α-amino groups of proteins and amino acids)
    • Color of nitrated products of aromatic rings: Yellow

    Chemical Reactions

    • Reaction with basic sodium hydroxide: Solution turns red
    • Reaction with glyoxylic acid and concentrated sulfuric acid (Hopkins-Cole test): Forms a purple complex
    • Reaction with nitric acid: Forms a yellow nitrated product

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    Description

    Learn about Millon’s Test, an analytical method used to detect the amino acid tyrosine by nitrification of the phenol group and formation of complexes with heavy metals. Understand its specificity and its application in identifying tyrosine in proteins.

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