Podcast
Questions and Answers
What is the purpose of Millon's test?
What is the purpose of Millon's test?
- To detect the presence of heavy metals in proteins
- To detect the presence of all amino acids in proteins
- To detect the presence of tyrosine in proteins (correct)
- To detect the presence of phenol group in all compounds
What is the composition of Millon's reagent?
What is the composition of Millon's reagent?
- Sodium nitrate and concentrated sulfuric acid
- Calcium carbonate and acetic acid
- Mercuric nitrate and mercurous nitrate dissolved in concentrated nitric acid (correct)
- Potassium chloride and hydrochloric acid
What happens to the tyrosine molecule during Millon's test?
What happens to the tyrosine molecule during Millon's test?
- It reacts with nitric acid to form a yellow-colored precipitate
- It undergoes nitrification to form a green-colored solution
- It forms complexes with heavy metals like mercury (correct)
- It combines with sulfuric acid to produce a white-colored substance
Is Millon's test specific for detecting tyrosine in proteins?
Is Millon's test specific for detecting tyrosine in proteins?
What is the color of the precipitate or solution formed during Millon's test?
What is the color of the precipitate or solution formed during Millon's test?
Which group of compounds does Millon's test specifically detect?
Which group of compounds does Millon's test specifically detect?
Which color complex is formed when ninhydrin reacts with α-amino groups of proteins and amino acids?
Which color complex is formed when ninhydrin reacts with α-amino groups of proteins and amino acids?
What color do nitrated products of aromatic rings on the side chains of proteins or free amino acids appear as?
What color do nitrated products of aromatic rings on the side chains of proteins or free amino acids appear as?
In Millon's test, what color does the solution turn into when made with basic sodium hydroxide?
In Millon's test, what color does the solution turn into when made with basic sodium hydroxide?
Which protein group specifically answers the Xanthoproteic test?
Which protein group specifically answers the Xanthoproteic test?
What is the color of the complex formed in the Hopkins-Cole test upon reaction with glyoxylic acid and concentrated sulfuric acid?
What is the color of the complex formed in the Hopkins-Cole test upon reaction with glyoxylic acid and concentrated sulfuric acid?
What kind of product does the presence of nitric acid lead to in proteins or free amino acids?
What kind of product does the presence of nitric acid lead to in proteins or free amino acids?
What color complex is typically formed when α-amino acids react with ninhydrin?
What color complex is typically formed when α-amino acids react with ninhydrin?
What color do nitrated products of aromatic rings on the side chains of proteins or free amino acids appear as in solution?
What color do nitrated products of aromatic rings on the side chains of proteins or free amino acids appear as in solution?
What is the purpose of Millon's test for proteins?
What is the purpose of Millon's test for proteins?
What is the color of the complex formed in the Hopkins-Cole test upon reaction with glyoxylic acid and concentrated sulfuric acid?
What is the color of the complex formed in the Hopkins-Cole test upon reaction with glyoxylic acid and concentrated sulfuric acid?
What happens to the color of the solution in Millon's test when it is made with basic sodium hydroxide?
What happens to the color of the solution in Millon's test when it is made with basic sodium hydroxide?
What kind of product is formed when aromatic rings on the side chains of proteins or free amino acids are treated with concentrated nitric acid?
What kind of product is formed when aromatic rings on the side chains of proteins or free amino acids are treated with concentrated nitric acid?
Study Notes
Millon's Test
- Purpose: To detect the presence of tyrosine in proteins
- Composition of Millon's reagent: Mercury(II) sulfate and sulfuric acid
- Tyrosine molecule: Forms a red precipitate when treated with Millon's reagent
- Specificity: Millon's test is not specific for tyrosine and can also detect other phenolic compounds
Reaction Products
- Color of the precipitate or solution formed: Red
- Compounds detected: Phenolic compounds, including tyrosine
- Color complex formed with ninhydrin: Purple (with α-amino groups of proteins and amino acids)
- Color of nitrated products of aromatic rings: Yellow
Chemical Reactions
- Reaction with basic sodium hydroxide: Solution turns red
- Reaction with glyoxylic acid and concentrated sulfuric acid (Hopkins-Cole test): Forms a purple complex
- Reaction with nitric acid: Forms a yellow nitrated product
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Description
Learn about Millon’s Test, an analytical method used to detect the amino acid tyrosine by nitrification of the phenol group and formation of complexes with heavy metals. Understand its specificity and its application in identifying tyrosine in proteins.
