Microtomy Techniques in Histology
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Questions and Answers

What is the purpose of placing a tissue slice in a water bath after cutting?

  • To stain the tissue
  • To flatten out the tissue (correct)
  • To dry the tissue
  • To dehydrate the tissue

What type of microtome is used for rapid diagnosis?

  • Celloidin Microtome
  • Paraffin Microtome
  • Freezing Microtome (correct)
  • Ultrathin Microtome

What is the purpose of using carbon dioxide in the Freezing Microtome?

  • To freeze the tissue (correct)
  • To dry the tissue
  • To melt the tissue
  • To stain the tissue

What type of sections can be obtained using an Ultrathin Microtome?

<p>Semi-thin sections (D)</p> Signup and view all the answers

What is the advantage of using a Freezing Microtome for certain tissue constituents?

<p>It preserves heat-labile tissue constituents (B)</p> Signup and view all the answers

What is the primary function of the valve/tubing in the Freezing Microtome?

<p>To release carbon dioxide to freeze the tissue (B)</p> Signup and view all the answers

Why would the block edges need to be adjusted to be parallel to the knife?

<p>To enable smooth cutting of the sections (B)</p> Signup and view all the answers

What is the purpose of adhesives in the mounting process?

<p>To promote adhesion of sections to the slides (C)</p> Signup and view all the answers

What may happen if delicate tissues (such as CNS tissues) are not treated with special care?

<p>They will split and crack due to excess heat (A)</p> Signup and view all the answers

What is the recommended temperature for drying slides with delicate tissues?

<p>37°C for 24 hours (B)</p> Signup and view all the answers

Why may the block need to be re-embedded in freshly filtered wax?

<p>The wax has become contaminated (D)</p> Signup and view all the answers

What is the final step in tissue processing?

<p>Mounting (D)</p> Signup and view all the answers

What is the primary purpose of microtomy in tissue processing?

<p>To facilitate studies under a microscope (A)</p> Signup and view all the answers

Which part of the microtome is responsible for adjusting the proper thickness of the tissue for successive sections?

<p>Adjustment screws (C)</p> Signup and view all the answers

What is the purpose of the ratchet feed wheel in a microtome?

<p>To move the tissue block towards the knife (D)</p> Signup and view all the answers

Which type of microtome is used for cutting serial sections of large blocks of paraffin embedded tissues?

<p>Rocking microtome (D)</p> Signup and view all the answers

What is the main function of the block holder in a microtome?

<p>To hold the tissue block in position (D)</p> Signup and view all the answers

What is the principle of operation of a rotary microtome?

<p>Rotating the ratchet feed wheel to cut the sections (B)</p> Signup and view all the answers

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