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Microtomy Techniques in Histology

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Questions and Answers

What is the purpose of placing a tissue slice in a water bath after cutting?

To stain the tissue

To flatten out the tissue (correct)

To dry the tissue

To dehydrate the tissue

What type of microtome is used for rapid diagnosis?

Celloidin Microtome

Paraffin Microtome

Freezing Microtome (correct)

Ultrathin Microtome

What is the purpose of using carbon dioxide in the Freezing Microtome?

To freeze the tissue (correct)

To dry the tissue

To melt the tissue

To stain the tissue

What type of sections can be obtained using an Ultrathin Microtome?

Semi-thin sections Signup and view all the answers

What is the advantage of using a Freezing Microtome for certain tissue constituents?

It preserves heat-labile tissue constituents Signup and view all the answers

What is the primary function of the valve/tubing in the Freezing Microtome?

To release carbon dioxide to freeze the tissue Signup and view all the answers

Why would the block edges need to be adjusted to be parallel to the knife?

To enable smooth cutting of the sections Signup and view all the answers

What is the purpose of adhesives in the mounting process?

To promote adhesion of sections to the slides Signup and view all the answers

What may happen if delicate tissues (such as CNS tissues) are not treated with special care?

They will split and crack due to excess heat Signup and view all the answers

What is the recommended temperature for drying slides with delicate tissues?

37°C for 24 hours Signup and view all the answers

Why may the block need to be re-embedded in freshly filtered wax?

The wax has become contaminated Signup and view all the answers

What is the final step in tissue processing?

Mounting Signup and view all the answers

What is the primary purpose of microtomy in tissue processing?

To facilitate studies under a microscope Signup and view all the answers

Which part of the microtome is responsible for adjusting the proper thickness of the tissue for successive sections?

Adjustment screws Signup and view all the answers

What is the purpose of the ratchet feed wheel in a microtome?

To move the tissue block towards the knife Signup and view all the answers

Which type of microtome is used for cutting serial sections of large blocks of paraffin embedded tissues?

Rocking microtome Signup and view all the answers

What is the main function of the block holder in a microtome?

To hold the tissue block in position Signup and view all the answers

What is the principle of operation of a rotary microtome?

Rotating the ratchet feed wheel to cut the sections Signup and view all the answers