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Questions and Answers
What is the function of the cassette clamp or block holder in a rotary microtome?
What is the function of the cassette clamp or block holder in a rotary microtome?
- It holds the paraffin block in place and moves up and down with each revolution. (correct)
- It moves the block holder either toward the knife or away from the knife.
- It adjusts the micron settings for section thickness.
- It turns in one direction and advances the block toward the knife at specified microns.
Which part of the rotary microtome moves the block holder toward or away from the knife?
Which part of the rotary microtome moves the block holder toward or away from the knife?
- Micron adjustment
- Coarse hand wheel (correct)
- Cassette clamp
- Advancement hand wheel
What is the purpose of the advancement hand wheel in a rotary microtome?
What is the purpose of the advancement hand wheel in a rotary microtome?
- To adjust the micron settings for section thickness
- To hold the paraffin block in place
- To advance the block toward the knife at the specified microns (correct)
- To lock the knife in place for safety
Why is a safety lock important for the advancement hand wheel?
Why is a safety lock important for the advancement hand wheel?
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What is the range of micron settings for section thickness on most microtomes?
What is the range of micron settings for section thickness on most microtomes?
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What part of the rotary microtome provides support for the entire instrument?
What part of the rotary microtome provides support for the entire instrument?
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Study Notes
Rotary Microtome Parts
Cassette Clamp or Block Holder
- Holds the paraffin block in place and moves up and down with each revolution while the blade is stationary.
- May have knobs that allow the user to manipulate the block face in various directions to align the tissue with the blade.
Coarse Hand Wheel
- Moves the block holder towards or away from the knife.
Advancement Hand Wheel
- Turns in one direction to advance the block towards the knife at specified microns.
- Equipped with a safety lock to prevent the wheel from releasing and having the block holder come down towards the blade while a block is inserted or removed.
- The safety lock should be used when not actively sectioning paraffin blocks.
Micron Adjustment
- Allows for section thickness settings ranging from 1 to 60 microns on most microtomes.
Base
- The base on which the entire microtome rests.
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