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6 Questions

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Rotary Microtome Parts Identification

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Questions and Answers

What is the function of the cassette clamp or block holder in a rotary microtome?

It holds the paraffin block in place and moves up and down with each revolution. (correct)

It moves the block holder either toward the knife or away from the knife.

It adjusts the micron settings for section thickness.

It turns in one direction and advances the block toward the knife at specified microns.

Which part of the rotary microtome moves the block holder toward or away from the knife?

Micron adjustment

Coarse hand wheel (correct)

Cassette clamp

Advancement hand wheel

What is the purpose of the advancement hand wheel in a rotary microtome?

To adjust the micron settings for section thickness

To hold the paraffin block in place

To advance the block toward the knife at the specified microns (correct)

To lock the knife in place for safety

Why is a safety lock important for the advancement hand wheel?

It stops the block holder from coming down towards the blade when not actively sectioning. Signup and view all the answers

What is the range of micron settings for section thickness on most microtomes?

1 to 60 microns Signup and view all the answers

What part of the rotary microtome provides support for the entire instrument?

Base Signup and view all the answers

Study Notes

Rotary Microtome Parts

Cassette Clamp or Block Holder

Holds the paraffin block in place and moves up and down with each revolution while the blade is stationary.
May have knobs that allow the user to manipulate the block face in various directions to align the tissue with the blade.

Coarse Hand Wheel

Moves the block holder towards or away from the knife.

Advancement Hand Wheel

Turns in one direction to advance the block towards the knife at specified microns.
Equipped with a safety lock to prevent the wheel from releasing and having the block holder come down towards the blade while a block is inserted or removed.
The safety lock should be used when not actively sectioning paraffin blocks.