Microscopy Techniques Quiz

Phase contrast

Hemispherical with internal rays emanating radially

Confusing and disturbed by phase halos outside the plane of focus

<p>Transmitted DIC</p> Signup and view all the answers

<p>Changes in color and contrast</p> Signup and view all the answers

<p>Confused by halos around annular rings and within the stem</p> Signup and view all the answers

<p>Transparent specimens</p> Signup and view all the answers

<p>It allows visualization of unstained specimens</p> Signup and view all the answers

<p>By showing the orientation of molecules inside living cells, tissues, and organisms</p> Signup and view all the answers

<p>To emit fluorescence when excited by a high-intensity light source</p> Signup and view all the answers

<p>By providing contrast to transparent specimens without staining</p> Signup and view all the answers

<p>Fibers, hairs, paints, and glass fragments</p> Signup and view all the answers

<p>Dark field microscopy illuminates the specimen with light that is scattered by the specimen, while bright field microscopy illuminates the specimen directly.</p> Signup and view all the answers

<p>Phase contrast microscopy is suitable for extremely thin, transparent specimens that are usually not visible with brightfield microscopy.</p> Signup and view all the answers

<p>DIC microscopy creates image contrast by using two coherent beams of light and gradients in optical path.</p> Signup and view all the answers

<p>DIC microscopy allows the microscope to achieve excellent resolution.</p> Signup and view all the answers

<p>Fluorescence microscopy relies on the fluorescent properties of specific molecules in the specimen to visualize it.</p> Signup and view all the answers

<p>Polarized microscopy enhances image contrast by filtering out light waves that are not aligned with the polarizer.</p> Signup and view all the answers