Light Microscopy Techniques Quiz

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Questions and Answers

What technique allows researchers to transfect cells with labeled proteins for visualization?

  • Phase contrast
  • Immunostaining
  • Confocal microscopy
  • Transfecting with labeled protein (correct)

In what way does confocal microscopy differ from traditional optical microscopes?

  • Confocal microscopy can only produce 2D images
  • Confocal microscopy cannot visualize fluorescent proteins
  • Confocal microscopy has a lower magnification power
  • Confocal microscopy produces optical sections by excluding out-of-focus light (correct)

What is the main advantage of using immunofluorescence in microscopy?

  • Lower cost of reagents
  • Higher magnification capability
  • Enhanced structural resolution
  • Visualizing multiple proteins simultaneously (correct)

Which technique is best suited for observing neural circuits in the brain using a rainbow of colors?

<p>Live multispectral imaging (A)</p> Signup and view all the answers

What do the green mice and red fluorescence in cancers represent in the context of fluorescence microscopy?

<p>Angiogenesis in brain tumor blood vessels (B)</p> Signup and view all the answers

Which imaging technique is most useful for observing complex three-dimensional objects?

<p>Confocal microscopy (C)</p> Signup and view all the answers

What is the purpose of using fluorescent proteins like GFP in live organisms during imaging?

<p>To visualize specific proteins or structures in living samples (B)</p> Signup and view all the answers

1

<p>Five (C)</p> Signup and view all the answers

How many animals were mentioned to be using an imaging method?

<p>Five (C)</p> Signup and view all the answers

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