Microscopy Staining Techniques Quiz

Dyes are colored organic compounds that can selectively bind to tissues. Most modern dyes are synthesized from simpler organic molecules, usually benzene or one of its derivatives. Examples of simple organic compounds from which modern dyes are synthesized include alkanes, benzene, and alcohols.

A coloring agent used for general purposes is called a dye, while one used for biological purposes is called a stain. Stains are used in microscopy to enhance contrast in the microscopic image in biology and medicine to highlight structures in cell populations or organelles within individual cells, while dyes are used for general coloring purposes.

Any group that makes an organic compound colored is called a chromophore. Organic compounds such as alkanes, benzene, and alcohols are colorless to the human eye but will absorb light outside the visible spectrum. Benzene, for example, absorbs strongly in the UV region of the spectrum but appears water-white to the human eye. Benzene can be made to absorb visible light by adding a suitable chromophore.

<p>Staining is a technique used in microscopy to enhance contrast in the microscopic image in biology and medicine to highlight structures in cell populations or organelles within individual cells.</p> Signup and view all the answers

<p>The staining process may involve immersing the sample in dye solution, followed by rinsing and observation. Many dyes require the use of a mordant, which is a chemical compound that reacts with the stain to form an insoluble, colored precipitate. When excess dye solution is washed away, the mordant stain remains. Stains are generally prepared largely as aqueous solutions.</p> Signup and view all the answers

<p>A coloring agent used for general purposes is called a dye, while a coloring agent used for biological purposes is called a stain.</p> Signup and view all the answers

<p>Most modern dyes are synthesized from simpler organic molecules, usually benzene or one of its derivatives.</p> Signup and view all the answers

<p>A mordant is a chemical compound that reacts with the stain to form an insoluble, colored precipitate. It is used to help the stain bind to the sample and remain after excess dye solution is washed away.</p> Signup and view all the answers

<p>A group that makes an organic compound colored is called a chromophore. Benzene can be made to absorb visible light by adding a suitable chromophore.</p> Signup and view all the answers

<p>The staining process may involve immersing the sample in dye solution, followed by rinsing and observation. Some dyes require the use of a mordant, and stains are generally prepared as aqueous solutions.</p> Signup and view all the answers